Imperial College London
Alternate

ProfessorClareLloyd

Faculty of Medicine, National Heart & Lung Institute

Interim Head of NHLI, Vice-Dean (institutional Affairs) FoM
 
 
 
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Contact

 

+44 (0)20 7594 3102c.lloyd Website

 
 
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Location

 

Office 352Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Stolting:2022:10.1038/s41385-022-00545-4,
author = {Stolting, H and Baillon, L and Frise, R and Bonner, K and Hewitt, RJ and Molyneaux, PL and Gore, ML and Barclay, WS and Saglani, S and Lloyd, CM},
doi = {10.1038/s41385-022-00545-4},
journal = {Mucosal Immunology},
pages = {952--963},
title = {Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1},
url = {http://dx.doi.org/10.1038/s41385-022-00545-4},
volume = {15},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Children are less likely than adults to suffer severe symptoms when infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), while influenza A H1N1 severity is comparable across ages except for the very young or elderly. Airway epithelial cells play a vital role in the early defence against viruses via their barrier and immune functions. We investigated viral replication and immune responses in SARS-CoV-2-infected bronchial epithelial cells from healthy paediatric (n = 6; 2.5–5.6 years old) and adult (n = 4; 47–63 years old) subjects and compared cellular responses following infection with SARS-CoV-2 or Influenza A H1N1. While infection with either virus triggered robust transcriptional interferon responses, including induction of type I (IFNB1) and type III (IFNL1) interferons, markedly lower levels of interferons and inflammatory proteins (IL-6, IL-8) were released following SARS-CoV-2 compared to H1N1 infection. Only H1N1 infection caused disruption of the epithelial layer. Interestingly, H1N1 infection resulted in sustained upregulation of SARS-CoV-2 entry factors FURIN and NRP1. We did not find any differences in the epithelial response to SARS-CoV-2 infection between paediatric and adult cells. Overall, SARS-CoV-2 had diminished potential to replicate, affect morphology and evoke immune responses in bronchial epithelial cells compared to H1N1.
AU  - Stolting,H
AU  - Baillon,L
AU  - Frise,R
AU  - Bonner,K
AU  - Hewitt,RJ
AU  - Molyneaux,PL
AU  - Gore,ML
AU  - Barclay,WS
AU  - Saglani,S
AU  - Lloyd,CM
DO  - 10.1038/s41385-022-00545-4
EP  - 963
PY  - 2022///
SN  - 1933-0219
SP  - 952
TI  - Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1
T2  - Mucosal Immunology
UR  - http://dx.doi.org/10.1038/s41385-022-00545-4
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000825923800001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/98447
VL  - 15
ER  -
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