Imperial College London

ProfessorCristinaLo Celso

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Stem Cell Biology
 
 
 
//

Contact

 

+44 (0)20 7594 5359c.lo-celso

 
 
//

Location

 

550Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Willis:2018:10.1128/mBio.00933-18,
author = {Willis, A and Lo, Celso C and Filloux, A and Torraca, V and Mazon, Moya M and Castro, Gomes M and Shelley, J and Mostowy, S},
doi = {10.1128/mBio.00933-18},
journal = {mBio},
title = {Shigella-induced emergency granulopoiesis protects zebrafish larvae from secondary infection},
url = {http://dx.doi.org/10.1128/mBio.00933-18},
volume = {9},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Emergency granulopoiesis is a hematopoietic program of stem cell-driven neutrophil production used to counteract immune cell exhaustion following infection. Shigella flexneri is a Gram-negative enteroinvasive pathogen controlled by neutrophils. In this study, we use a Shigella-zebrafish (Danio rerio) infection model to investigate emergency granulopoiesis in vivo. We show that stem cell-driven neutrophil production occurs in response to Shigella infection and requires macrophage-independent signaling by granulocyte colony-stimulating factor (Gcsf). To test whether emergency granulopoiesis can function beyond homoeostasis to enhance innate immunity, we developed a reinfection assay using zebrafish larvae that have not yet developed an adaptive immune system. Strikingly, larvae primed with a sublethal dose of Shigella are protected against a secondary lethal dose of Shigella in a type III secretion system (T3SS)-dependent manner. Collectively, these results highlight a new role for emergency granulopoiesis in boosting host defense and demonstrate that zebrafish larvae can be a valuable in vivo model to investigate innate immune memory.IMPORTANCE Shigella is an important human pathogen of the gut. Emergency granulopoiesis is the enhanced production of neutrophils by hematopoietic stem and progenitor cells (HSPCs) upon infection and is widely considered a homoeostatic mechanism for replacing exhausted leukocytes. In this study, we developed a Shigella-zebrafish infection model to investigate stem cell-driven emergency granulopoiesis. We discovered that zebrafish initiate granulopoiesis in response to Shigella infection, via macrophage-independent signaling of granulocyte colony-stimulating factor (Gcsf). Strikingly, larvae primed with a sublethal dose of Shigella are protected against a secondary lethal dose of Shigella in a type III secretion system (T3SS)-dependent manner. Taken together, we show that zebrafish infection can be used to capture Shigella-mediated stem c
AU - Willis,A
AU - Lo,Celso C
AU - Filloux,A
AU - Torraca,V
AU - Mazon,Moya M
AU - Castro,Gomes M
AU - Shelley,J
AU - Mostowy,S
DO - 10.1128/mBio.00933-18
PY - 2018///
SN - 2150-7511
TI - Shigella-induced emergency granulopoiesis protects zebrafish larvae from secondary infection
T2 - mBio
UR - http://dx.doi.org/10.1128/mBio.00933-18
UR - http://hdl.handle.net/10044/1/60466
VL - 9
ER -