Imperial College London

ProfessorCristinaLo Celso

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Stem Cell Biology
 
 
 
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Contact

 

+44 (0)20 7594 5359c.lo-celso

 
 
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Location

 

550Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Scott:2014:10.3791/51683,
author = {Scott, MK and Akinduro, O and Lo, Celso C},
doi = {10.3791/51683},
journal = {Jove-Journal of Visualized Experiments},
title = {In vivo 4-dimensional tracking of hematopoietic stem and progenitor cells in adult mouse calvarial bone marrow},
url = {http://dx.doi.org/10.3791/51683},
year = {2014}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Through a delicate balance between quiescence and proliferation, self renewal and production of differentiated progeny, hematopoietic stem cells (HSCs) maintain the turnover of all mature blood cell lineages. The coordination of the complex signals leading to specific HSC fates relies upon the interaction between HSCs and the intricate bone marrow microenvironment, which is still poorly understood[1-2].We describe how by combining a newly developed specimen holder for stable animal positioning with multi-step confocal and two-photon in vivo imaging techniques, it is possible to obtain high-resolution 3D stacks containing HSPCs and their surrounding niches and to monitor them over time through multi-point time-lapse imaging. High definition imaging allows detecting ex vivo labeled hematopoietic stem and progenitor cells (HSPCs) residing within the bone marrow. Moreover, multi-point time-lapse 3D imaging, obtained with faster acquisition settings, provides accurate information about HSPC movement and the reciprocal interactions between HSPCs and stroma cells.Tracking of HSPCs in relation to GFP positive osteoblastic cells is shown as an exemplary application of this method. This technique can be utilized to track any appropriately labeled hematopoietic or stromal cell of interest within the mouse calvarium bone marrow space.
AU - Scott,MK
AU - Akinduro,O
AU - Lo,Celso C
DO - 10.3791/51683
PY - 2014///
SN - 1940-087X
TI - In vivo 4-dimensional tracking of hematopoietic stem and progenitor cells in adult mouse calvarial bone marrow
T2 - Jove-Journal of Visualized Experiments
UR - http://dx.doi.org/10.3791/51683
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000349301100023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/57565
ER -