Imperial College London
Alternate

ProfessorCristinaLo Celso

Faculty of Natural Sciences, Department of Life Sciences

Co-Director Centre for Haematology & Prof Stem Cell Biology
 
 
 
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Contact

 

c.lo-celso

 
 
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Location

 

548Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Silberstein:2016:10.1016/j.stem.2016.07.004,
author = {Silberstein, L and Goncalves, KA and Kharchenko, PV and Turcotte, R and Kfoury, Y and Mercier, F and Baryawno, N and Severe, N and Bachand, J and Spencer, JA and Papazian, A and Lee, D and Chitteti, BR and Srour, EF and Hoggatt, J and Tate, T and Lo, Celso C and Ono, N and Nutt, S and Heino, J and Sipilä, K and Shioda, T and Osawa, M and Lin, CP and Hu, G-F and Scadden, DT},
doi = {10.1016/j.stem.2016.07.004},
journal = {Cell Stem Cell},
pages = {530--543},
title = {Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators.},
url = {http://dx.doi.org/10.1016/j.stem.2016.07.004},
volume = {19},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Physiological stem cell function is regulated by secreted factors produced by niche cells. In this study, we describe an unbiased approach based on the differential single-cell gene expression analysis of mesenchymal osteolineage cells close to, and further removed from, hematopoietic stem/progenitor cells (HSPCs) to identify candidate niche factors. Mesenchymal cells displayed distinct molecular profiles based on their relative location. We functionally examined, among the genes that were preferentially expressed in proximal cells, three secreted or cell-surface molecules not previously connected to HSPC biology-the secreted RNase angiogenin, the cytokine IL18, and the adhesion molecule Embigin-and discovered that all of these factors are HSPC quiescence regulators. Therefore, our proximity-based differential single-cell approach reveals molecular heterogeneity within niche cells and can be used to identify novel extrinsic stem/progenitor cell regulators. Similar approaches could also be applied to other stem cell/niche pairs to advance the understanding of microenvironmental regulation of stem cell function.
AU  - Silberstein,L
AU  - Goncalves,KA
AU  - Kharchenko,PV
AU  - Turcotte,R
AU  - Kfoury,Y
AU  - Mercier,F
AU  - Baryawno,N
AU  - Severe,N
AU  - Bachand,J
AU  - Spencer,JA
AU  - Papazian,A
AU  - Lee,D
AU  - Chitteti,BR
AU  - Srour,EF
AU  - Hoggatt,J
AU  - Tate,T
AU  - Lo,Celso C
AU  - Ono,N
AU  - Nutt,S
AU  - Heino,J
AU  - Sipilä,K
AU  - Shioda,T
AU  - Osawa,M
AU  - Lin,CP
AU  - Hu,G-F
AU  - Scadden,DT
DO  - 10.1016/j.stem.2016.07.004
EP  - 543
PY  - 2016///
SP  - 530
TI  - Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators.
T2  - Cell Stem Cell
UR  - http://dx.doi.org/10.1016/j.stem.2016.07.004
UR  - https://www.ncbi.nlm.nih.gov/pubmed/27524439
VL  - 19
ER  -
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