Publications
147 results found
van der Veen AG, Maillard PV, Reis e Sousa C, 2015, Drosha cuts the tethers of myelopoiesis, NATURE IMMUNOLOGY, Vol: 16, Pages: 1110-1112, ISSN: 1529-2908
- Author Web Link
- Cite
- Citations: 1
Yatim N, Jusforgues-Saklani H, Orozco S, et al., 2015, RIPK1 and NF-κB signaling in dying cells determines cross-priming of CD8<SUP>+</SUP> T cells, SCIENCE, Vol: 350, Pages: 328-+, ISSN: 0036-8075
- Author Web Link
- Cite
- Citations: 389
Zelenay S, van der Veen AG, Böttcher JP, et al., 2015, Cyclooxygenase-dependent tumor growth through evasion of immunity, Cell, Vol: 162, Pages: 1257-1270, ISSN: 0092-8674
The mechanisms by which melanoma and other cancer cells evade anti-tumor immunity remain incompletely understood. Here, we show that the growth of tumors formed by mutant Braf(V600E) mouse melanoma cells in an immunocompetent host requires their production of prostaglandin E2, which suppresses immunity and fuels tumor-promoting inflammation. Genetic ablation of cyclooxygenases (COX) or prostaglandin E synthases in Braf(V600E) mouse melanoma cells, as well as in Nras(G12D) melanoma or in breast or colorectal cancer cells, renders them susceptible to immune control and provokes a shift in the tumor inflammatory profile toward classic anti-cancer immune pathways. This mouse COX-dependent inflammatory signature is remarkably conserved in human cutaneous melanoma biopsies, arguing for COX activity as a driver of immune suppression across species. Pre-clinical data demonstrate that inhibition of COX synergizes with anti-PD-1 blockade in inducing eradication of tumors, implying that COX inhibitors could be useful adjuvants for immune-based therapies in cancer patients.
Helft J, Boettcher J, Chakravarty P, et al., 2015, GM-CSF mouse bone marrow cultures comprise a heterogeneous population of CD11c(+)MHCII(+) macrophages and dendritic cells, Immunity, Vol: 42, Pages: 1197-1211, ISSN: 1074-7613
Dendritic cells (DCs) are key players in the immune system. Much of their biology has been elucidated via culture systems in which hematopoietic precursors differentiate into DCs under the aegis of cytokines. A widely used protocol involves the culture of murine bone marrow (BM) cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) to generate BM-derived DCs (BMDCs). BMDCs express CD11c and MHC class II (MHCII) molecules and share with DCs isolated from tissues the ability to present exogenous antigens to T cells and to respond to microbial stimuli by undergoing maturation. We demonstrate that CD11c+MHCII+ BMDCs are in fact a heterogeneous group of cells that comprises conventional DCs and monocyte-derived macrophages. DCs and macrophages in GM-CSF cultures both undergo maturation upon stimulation with lipopolysaccharide but respond differentially to the stimulus and remain separable entities. These results have important implications for the interpretation of a vast array of data obtained with DC culture systems.
Hipp MM, Shepherd D, Booth S, et al., 2015, The Processed Amino-Terminal Fragment of Human TLR7 Acts as a Chaperone To Direct Human TLR7 into Endosomes, JOURNAL OF IMMUNOLOGY, Vol: 194, Pages: 5417-5425, ISSN: 0022-1767
- Author Web Link
- Cite
- Citations: 13
Hanc P, Fujii T, Iborra S, et al., 2015, Structure of the Complex of F-Actin and DNGR-1, a C-Type Lectin Receptor Involved in Dendritic Cell Cross-Presentation of Dead Cell-Associated Antigens, IMMUNITY, Vol: 42, Pages: 839-849, ISSN: 1074-7613
- Author Web Link
- Cite
- Citations: 51
Swamy M, Abeler-Doerner L, Chettle J, et al., 2015, Intestinal intraepithelial lymphocyte activation promotes innate antiviral resistance, NATURE COMMUNICATIONS, Vol: 6, ISSN: 2041-1723
- Author Web Link
- Cite
- Citations: 53
Li J, Ahmet F, Sullivan LC, et al., 2015, Antibodies targeting Clec9A promote strong humoral immunity without adjuvant in mice and non-human primates, EUROPEAN JOURNAL OF IMMUNOLOGY, Vol: 45, Pages: 854-864, ISSN: 0014-2980
- Author Web Link
- Cite
- Citations: 60
Gaya M, Castello A, Montaner B, et al., 2015, Inflammation-induced disruption of SCS macrophages impairs B cell responses to secondary infection, SCIENCE, Vol: 347, Pages: 667-672, ISSN: 0036-8075
- Author Web Link
- Cite
- Citations: 97
Schraml BU, Reis e Sousa C, 2015, Defining dendritic cells, CURRENT OPINION IN IMMUNOLOGY, Vol: 32, Pages: 13-20, ISSN: 0952-7915
- Author Web Link
- Cite
- Citations: 133
van Blijswijk J, Schraml BU, Rogers NC, et al., 2015, Altered Lymph Node Composition in Diphtheria Toxin Receptor-Based Mouse Models To Ablate Dendritic Cells, JOURNAL OF IMMUNOLOGY, Vol: 194, Pages: 307-315, ISSN: 0022-1767
- Author Web Link
- Cite
- Citations: 14
Swamy M, Abeler-Doerner L, Goubau D, et al., 2014, Intraepithelial lymphocytes prime the innate antiviral response of the intestinal epithelium through type I and III interferons, IMMUNOLOGY, Vol: 143, Pages: 103-103, ISSN: 0019-2805
Acton SE, Farrugia AJ, Astarita JL, et al., 2014, Dendritic cells control fibroblastic reticular network tension and lymph node expansion, Nature, Vol: 514, Pages: 498-502, ISSN: 0028-0836
After immunogenic challenge, infiltrating and dividing lymphocytes markedly increase lymph node cellularity, leading to organ expansion1,2. Here we report that the physical elasticity of lymph nodes is maintained in part by podoplanin (PDPN) signalling in stromal fibroblastic reticular cells (FRCs) and its modulation by CLEC-2 expressed on dendritic cells. We show in mouse cells that PDPN induces actomyosin contractility in FRCs via activation of RhoA/C and downstream Rho-associated protein kinase (ROCK). Engagement by CLEC-2 causes PDPN clustering and rapidly uncouples PDPN from RhoA/C activation, relaxing the actomyosin cytoskeleton and permitting FRC stretching. Notably, administration of CLEC-2 protein to immunized mice augments lymph node expansion. In contrast, lymph node expansion is significantly constrained in mice selectively lacking CLEC-2 expression in dendritic cells. Thus, the same dendritic cells that initiate immunity by presenting antigens to T lymphocytes3 also initiate remodelling of lymph nodes by delivering CLEC-2 to FRCs. CLEC-2 modulation of PDPN signalling permits FRC network stretching and allows for the rapid lymph node expansion—driven by lymphocyte influx and proliferation—that is the critical hallmark of adaptive immunity.
Goubau D, Schlee M, Deddouche S, et al., 2014, Antiviral immunity via RIG-I-mediated recognition of RNA bearing 5 '-diphosphates, Nature, Vol: 514, Pages: 372-375, ISSN: 0028-0836
Mammalian cells possess mechanisms to detect and defend themselves from invading viruses. In the cytosol, the RIG-I-like receptors (RLRs), RIG-I (retinoic acid-inducible gene I; encoded by DDX58) and MDA5 (melanoma differentiation-associated gene 5; encoded by IFIH1) sense atypical RNAs associated with virus infection1,2. Detection triggers a signalling cascade via the adaptor MAVS that culminates in the production of type I interferons (IFN-α and β; hereafter IFN), which are key antiviral cytokines. RIG-I and MDA5 are activated by distinct viral RNA structures and much evidence indicates that RIG-I responds to RNAs bearing a triphosphate (ppp) moiety in conjunction with a blunt-ended, base-paired region at the 5′-end (reviewed in refs 1, 2, 3). Here we show that RIG-I also mediates antiviral responses to RNAs bearing 5′-diphosphates (5′pp). Genomes from mammalian reoviruses with 5′pp termini, 5′pp-RNA isolated from yeast L-A virus, and base-paired 5′pp-RNAs made by in vitro transcription or chemical synthesis, all bind to RIG-I and serve as RIG-I agonists. Furthermore, a RIG-I-dependent response to 5′pp-RNA is essential for controlling reovirus infection in cultured cells and in mice. Thus, the minimal determinant for RIG-I recognition is a base-paired RNA with 5′pp. Such RNAs are found in some viruses but not in uninfected cells, indicating that recognition of 5′pp-RNA, like that of 5′ppp-RNA, acts as a powerful means of self/non-self discrimination by the innate immune system.
Whitney PG, Baer E, Osorio F, et al., 2014, Syk Signaling in Dendritic Cells Orchestrates Innate Resistance to Systemic Fungal Infection, PLOS PATHOGENS, Vol: 10, ISSN: 1553-7366
- Author Web Link
- Cite
- Citations: 70
Deddouche S, Goubau D, Rehwinkel J, et al., 2014, Identification of an LGP2-associated MDA5 agonist in picornavirus-infected cells, ELIFE, Vol: 3, ISSN: 2050-084X
- Author Web Link
- Cite
- Citations: 93
Reis e Sousa C, Unanue ER, 2014, Antigen processing, CURRENT OPINION IN IMMUNOLOGY, Vol: 26, Pages: 138-139, ISSN: 0952-7915
- Author Web Link
- Cite
- Citations: 3
Baer E, Whitney PG, Moor K, et al., 2014, IL-17 Regulates Systemic Fungal Immunity by Controlling the Functional Competence of NK Cells, IMMUNITY, Vol: 40, Pages: 117-127, ISSN: 1074-7613
- Author Web Link
- Cite
- Citations: 141
Hipp MM, Shepherd D, Gileadi U, et al., 2013, Processing of Human Toll-like Receptor 7 by Furin-like Proprotein Convertases Is Required for Its Accumulation and Activity in Endosomes, 100th Annual Meeting of the American-Association-of-Immunologists, Publisher: CELL PRESS, Pages: 711-721, ISSN: 1074-7613
- Author Web Link
- Cite
- Citations: 63
Rehwinkel J, Maelfait J, Bridgeman A, et al., 2013, SAMHD1-dependent retroviral control and escape in mice, EMBO JOURNAL, Vol: 32, Pages: 2454-2462, ISSN: 0261-4189
- Author Web Link
- Cite
- Citations: 116
Rehwinkel J, Maelfait J, Rigby R, et al., 2013, SAMHD1-dependent retroviral control and escape in mice, Retrovirology, Vol: 10
Schraml BU, van Blijswijk J, Zelenay S, et al., 2013, Genetic Tracing via DNGR-1 Expression History Defines Dendritic Cells as a Hematopoietic Lineage, CELL, Vol: 154, Pages: 843-858, ISSN: 0092-8674
- Author Web Link
- Cite
- Citations: 217
van Blijswijk J, Schraml BU, Reis e Sousa C, 2013, Advantages and limitations of mouse models to deplete dendritic cells, EUROPEAN JOURNAL OF IMMUNOLOGY, Vol: 43, Pages: 22-26, ISSN: 0014-2980
- Author Web Link
- Cite
- Citations: 45
Tamoutounour S, Henri S, Lelouard H, et al., 2012, CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis, EUROPEAN JOURNAL OF IMMUNOLOGY, Vol: 42, Pages: 3150-3166, ISSN: 0014-2980
- Author Web Link
- Cite
- Citations: 376
Iborra S, Izquierdo HM, Martinez-Lopez M, et al., 2012, A dendritic cell receptor for damaged cells contributes to anti-viral immunity, European Congress of Immunology, Publisher: WILEY-BLACKWELL, Pages: 34-34, ISSN: 0019-2805
Poulin LF, Reyal Y, Uronen-Hansson H, et al., 2012, DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues, BLOOD, Vol: 119, Pages: 6052-6062, ISSN: 0006-4971
- Author Web Link
- Cite
- Citations: 185
Iborra S, Izquierdo HM, Martinez-Lopez M, et al., 2012, The DC receptor DNGR-1 mediates cross-priming of CTLs during vaccinia virus infection in mice, JOURNAL OF CLINICAL INVESTIGATION, Vol: 122, Pages: 1628-1643, ISSN: 0021-9738
- Author Web Link
- Cite
- Citations: 121
Zelenay S, Keller AM, Whitney PG, et al., 2012, The dendritic cell receptor DNGR-1 controls endocytic handling of necrotic cell antigens to favor cross-priming of CTLs in virus-infected mice, JOURNAL OF CLINICAL INVESTIGATION, Vol: 122, Pages: 1615-1627, ISSN: 0021-9738
- Author Web Link
- Cite
- Citations: 186
Ahrens S, Zelenay S, Sancho D, et al., 2012, F-Actin Is an Evolutionarily Conserved Damage-Associated Molecular Pattern Recognized by DNGR-1, a Receptor for Dead Cells, IMMUNITY, Vol: 36, Pages: 635-645, ISSN: 1074-7613
- Author Web Link
- Cite
- Citations: 284
Finney BA, Schweighoffer E, Navarro-Nunez L, et al., 2012, CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for development, BLOOD, Vol: 119, Pages: 1747-1756, ISSN: 0006-4971
- Author Web Link
- Cite
- Citations: 98
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.