Imperial College London
Professor Caetano Reis e Sousa

ProfessorCaetanoReis e Sousa

Faculty of Medicine, Department of Immunology and Inflammation

Visiting Professor
 
 
 
//

Contact

 

c.reisesousa

 
 
//

Location

 

Wright Fleming WingSt Mary's Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Schulz:2018:10.1016/j.celrep.2018.06.038,
author = {Schulz, O and Han, P and Böttcher, JP and Hoogeboom, R and Diebold, SS and Tolar, P and Reis, E Sousa C},
doi = {10.1016/j.celrep.2018.06.038},
journal = {Cell Reports},
pages = {419--428},
title = {Myosin II synergizes with F-actin to promote DNGR-1-dependent cross-presentation of dead cell-associated antigens},
url = {http://dx.doi.org/10.1016/j.celrep.2018.06.038},
volume = {24},
year = {2018}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Conventional type 1 DCs (cDC1s) excel at cross-presentation of dead cell-associated antigens partly because they express DNGR-1, a receptor that recognizes exposed actin filaments on dead cells. In vitro polymerized F-actin can be used as a synthetic ligand for DNGR-1. However, cellular F-actin is decorated with actin-binding proteins, which could affect DNGR-1 recognition. Here, we demonstrate that myosin II, an F-actin-associated motor protein, greatly potentiates the binding of DNGR-1 to F-actin. Latex beads coated with F-actin and myosin II are taken up by DNGR-1+ cDC1s, and antigen associated with those beads is efficiently cross-presented to CD8+ T cells. Myosin II-deficient necrotic cells are impaired in their ability to stimulate DNGR-1 or to serve as substrates for cDC1 cross-presentation to CD8+ T cells. These results provide insights into the nature of the DNGR-1 ligand and have implications for understanding immune responses to cell-associated antigens and for vaccine design.
AU  - Schulz,O
AU  - Han,P
AU  - Böttcher,JP
AU  - Hoogeboom,R
AU  - Diebold,SS
AU  - Tolar,P
AU  - Reis,E Sousa C
DO  - 10.1016/j.celrep.2018.06.038
EP  - 428
PY  - 2018///
SN  - 2211-1247
SP  - 419
TI  - Myosin II synergizes with F-actin to promote DNGR-1-dependent cross-presentation of dead cell-associated antigens
T2  - Cell Reports
UR  - http://dx.doi.org/10.1016/j.celrep.2018.06.038
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29996102
UR  - http://hdl.handle.net/10044/1/61262
VL  - 24
ER  -
Download