Imperial College London
Alternate

DrChristosRossios

Faculty of Medicine, National Heart & Lung Institute

Honorary Research Associate
 
 
 
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Contact

 

c.rossios05 Website

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kermani:2021:10.1186/s12931-020-01605-8,
author = {Kermani, N and Song, W-J and Badi, Y and Versi, A and Guo, Y and Sun, K and Bhavsar, P and Howarth, P and Dahlen, S-E and Sterk, PJ and Djukanovic, R and Adcock, I and Chung, KF},
doi = {10.1186/s12931-020-01605-8},
journal = {Respiratory Research},
title = {Sputum ACE2, TMPRSS2 and FURIN gene expression in severe neutrophilic asthma},
url = {http://dx.doi.org/10.1186/s12931-020-01605-8},
volume = {22},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundPatients with severe asthma may have a greater risk of dying from COVID-19 disease. Angiotensin converting enzyme-2 (ACE2) and the enzyme proteases, transmembrane protease serine 2 (TMPRSS2) and FURIN, are needed for viral attachment and invasion into host cells.MethodsWe examined microarray mRNA expression of ACE2, TMPRSS2 and FURIN in sputum, bronchial brushing and bronchial biopsies of the European U-BIOPRED cohort. Clinical parameters and molecular phenotypes, including asthma severity, sputum inflammatory cells, lung functions, oral corticosteroid (OCS) use, and transcriptomic-associated clusters, were examined in relation to gene expression levels.ResultsACE2 levels were significantly increased in sputum of severe asthma compared to mild-moderate asthma. In multivariate analyses, sputum ACE2 levels were positively associated with OCS use and male gender. Sputum FURIN levels were significantly related to neutrophils (%) and the presence of severe asthma. In bronchial brushing samples, TMPRSS2 levels were positively associated with male gender and body mass index, whereas FURIN levels with male gender and blood neutrophils. In bronchial biopsies, TMPRSS2 levels were positively related to blood neutrophils. The neutrophilic molecular phenotype characterised by high inflammasome activation expressed significantly higher FURIN levels in sputum than the eosinophilic Type 2-high or the pauci-granulocytic oxidative phosphorylation phenotypes.ConclusionLevels of ACE2 and FURIN may differ by clinical or molecular phenotypes of asthma. Sputum FURIN expression levels were strongly associated with neutrophilic inflammation and with inflammasome activation. This might indicate the potential for a greater morbidity and mortality outcome from SARS-CoV-2 infection in neutrophilic severe asthma.
AU  - Kermani,N
AU  - Song,W-J
AU  - Badi,Y
AU  - Versi,A
AU  - Guo,Y
AU  - Sun,K
AU  - Bhavsar,P
AU  - Howarth,P
AU  - Dahlen,S-E
AU  - Sterk,PJ
AU  - Djukanovic,R
AU  - Adcock,I
AU  - Chung,KF
DO  - 10.1186/s12931-020-01605-8
PY  - 2021///
SN  - 1465-9921
TI  - Sputum ACE2, TMPRSS2 and FURIN gene expression in severe neutrophilic asthma
T2  - Respiratory Research
UR  - http://dx.doi.org/10.1186/s12931-020-01605-8
UR  - http://hdl.handle.net/10044/1/86583
VL  - 22
ER  -
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