Imperial College London
Portrait Picture

DrChristopherRowlands

Faculty of Engineering, Department of Bioengineering

Senior Lecturer
 
 
 
//

Contact

 

+44 (0)20 7594 1331c.rowlands Website CV

 
 
//

Location

 

3.15Royal School of MinesSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@inbook{So:2018:10.1515/9783110429985-008,
author = {So, PTC and Choi, H and Yew, E and Rowlands, C},
booktitle = {Multiphoton Microscopy and Fluorescence Lifetime Imaging: Applications in Biology and Medicine},
doi = {10.1515/9783110429985-008},
pages = {103--140},
title = {Principles and applications of temporal-focusing wide-field two-photon microscopy},
url = {http://dx.doi.org/10.1515/9783110429985-008},
year = {2018}
}
Download

RIS format (EndNote, RefMan)

TY  - CHAP
AB  - Temporal focusing allows for rapid optically sectioned two-photon widefield microscopy. Depth sectioning is provided in a wide-field manner, without spatial focusing, by controlling the temporalwidth of femtosecond laser pulses near the focal plane. This spatial control of the temporal pulse width is achieved by diffracting the light off a grating resulting in spectral component separation and temporal broadening; these spectral components are only recombined at the focal plane to reproduce short, femtosecond pulses. Applications include (i) high speed functional imaging in the brain, (ii) fast FLIM and PLIM, (iii) cell-selective optogenetic excitation, and (iv) temporal focusing photodynamic therapy that may allow selective killing of cancer cells.
AU  - So,PTC
AU  - Choi,H
AU  - Yew,E
AU  - Rowlands,C
DO  - 10.1515/9783110429985-008
EP  - 140
PY  - 2018///
SN  - 9783110438987
SP  - 103
TI  - Principles and applications of temporal-focusing wide-field two-photon microscopy
T1  - Multiphoton Microscopy and Fluorescence Lifetime Imaging: Applications in Biology and Medicine
UR  - http://dx.doi.org/10.1515/9783110429985-008
ER  -
Download