Publications
302 results found
Watson SA, Perbellini F, Harding SE, et al., 2017, Myocardial Slices to Study the Structural and Functional Plasticity of the Myocardium Following Prolonged Changes in Mechanical Load in vitro, Scientific Sessions of the American-Heart-Association / Resuscitation Science Symposium, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7322
Wang BX, Kane C, Couch L, et al., 2017, Arginine-Glycine-Aspartic Acid-Motif Containing Peptides and Integrin Binding Modulate Human Induced Pluripotent Stem-Cell Derived Cardiomyocyte Calcium Cycling, Scientific Sessions of the American-Heart-Association / Resuscitation Science Symposium, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7322
Watson SA, Duff J, Perbellini F, et al., 2017, Sustained Mechanical Load Regulates Conduction Velocity, Connexin 43 Expression and Propensity to Arrhythmia in Rat Ultrathin Myocardial Slices, Scientific Sessions of the American-Heart-Association / Resuscitation Science Symposium, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7322
Wang B, Terracciano C, Macleod K, 2017, Regulation of cardiac excitation-contraction coupling by fibroblasts in health and disease, ISHR-NAS Annual Meeting, Publisher: ELSEVIER SCI LTD, Pages: 161-161, ISSN: 0022-2828
Terracciano C, 2017, Excitation-Contraction Coupling Plasticity in Pluripotent Stem Cell-Derived Cardiac Myocytes, Publisher: WILEY, Pages: 38-38, ISSN: 1748-1708
Perbellini F, Watson SA, Scigliano M, et al., 2017, Investigation of cardiac fibroblasts using myocardial slices, Cardiovascular Research, Vol: 114, Pages: 77-89, ISSN: 1755-3245
AimsCardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity are difficult to determine. When isolated and cultured in vitro, CFs undergo rapid phenotypic changes including increased expression of α-SMA. Here we describe a new model to study CFs and their response to pharmacological and mechanical stimuli using in vitro cultured mouse, dog and human myocardial slices.Methods and resultsUnloading of myocardial slices induced CF proliferation without α-SMA expression up to 7 days in culture. CFs migrating onto the culture plastic support or cultured on glass expressed αSMA within 3 days. The cells on the slice remained αSMA(−) despite transforming growth factor-β (20 ng/ml) or angiotensin II (200 µM) stimulation. When diastolic load was applied to myocardial slices using A-shaped stretchers, CF proliferation was significantly prevented at Days 3 and 7 (P < 0.001).ConclusionsMyocardial slices allow the study of CFs in a multicellular environment and may be used to effectively study mechanisms of cardiac fibrosis and potential targets.
Perbellini F, Bardi I, Watson S, et al., 2017, Cell therapy of the heart studied using adult myocardial slices in vitro, Annual Conference of the British-Society-for-Gene-and-Cell-Therapy / Joint UK-Regenerative-Medicine-Platform Meeting, Publisher: MARY ANN LIEBERT, INC, Pages: A19-A20, ISSN: 1043-0342
Humphrey EJ, Mazo MM, Amdursky N, et al., 2017, Abstract 342: Serum Albumin Hydrogels Alter Excitation-Contraction Coupling in Neonatal Rat Myocytes and Human Induced Pluripotent Stem Cell Derived Cardiomyocytes, Basic Cardiovascular Sciences Scientific Sessions of the American-Heart-Association - Pathways to Cardiovascular Therapeutics, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7330
Humphrey EJ, Kapnisi M, Mawad D, et al., 2017, Abstract 188: Manipulation of Excitation-contraction Coupling in Cardiomyocytes Using Conductive Polyaniline Scaffolds, Basic Cardiovascular Sciences Scientific Sessions of the American-Heart-Association - Pathways to Cardiovascular Therapeutics, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7330
Perbellini F, Liu AKL, Watson SA, et al., 2017, Free-of-acrylamide SDS-based tissue clearing (FASTClear) for three dimensional visualization of myocardial tissue, Scientific Reports, Vol: 7, ISSN: 2045-2322
Several pathologic conditions of the heart lead to cardiac structural remodelling. Given the high density and the opaque nature of the myocardium, deep three dimensional (3D) imaging is difficult to achieve and structural analysis of pathological myocardial structure is often limited to two dimensional images and of thin myocardial sections. Efficient methods to obtain optical clearing of the tissue for 3D visualisation are therefore needed. Here we describe a rapid, simple and versatile Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) protocol specifically designed for cardiac tissue. With this method 3D information regarding collagen content, collagen localization and distribution could be easily obtained across a whole 300 µm-thick myocardial slice. FASTClear does not induce structural or microstructural distortion and it can be combined with immunostaining to identify the micro- and macrovascular networks. In summary, we have obtained decolorized myocardial tissue suitable for high resolution 3D imaging, with implications for the study of complex cardiac tissue structure and its changes during pathology.
Guex AG, Spicer CD, Armgarth A, et al., 2017, Electrospun aniline-tetramer-co-polycaprolactone fibres for conductive, biodegradable scaffolds, MRS Communications, Vol: 7, Pages: 375-382, ISSN: 2159-6867
Conjugated polymers have been proposed as promising materials for scaffolds in tissue engineering applications. However, the restricted processability and biodegradability of conjugated polymers limit their use for biomedical applications. Here we synthesized a block-co-polymer of aniline tetramer and PCL (AT–PCL), and processed it into fibrous non-woven scaffolds by electrospinning. We showed that fibronectin (Fn) adhesion was dependent on the AT–PCL oxidative state, with a reduced Fn unfolding length on doped membranes. Furthermore, we demonstrated the cytocompatibility and potential of these membranes to support the growth and osteogenic differentiation of MC3T3-E1 cells over 21 days.
Mansfield C, Kapnisi M, Mawad D, et al., 2017, Characterising auxetic micropatterning of a conductive cardiac patch, Publisher: ELSEVIER SCI LTD, Pages: 29-29, ISSN: 0022-2828
Couch L, Clayton R, Derda AA, et al., 2017, Takotsubo syndrome associated miR-16 and miR-26a reduce contractility of apical cardiomyocytes in vitro by an inhibitory G-protein dependent mechanism, Publisher: ELSEVIER SCI LTD, Pages: 34-34, ISSN: 0022-2828
Kane C, Terracciano CMN, 2017, Criteria for chamber-specific categorisation of human cardiac myocytes derived from pluripotent stem cells, Stem Cells, Vol: 35, Pages: 1881-1897, ISSN: 1549-4918
Human pluripotent stem cell-derived cardiomyocytes (PSC-CMs) have great potential application in almost all areas of cardiovascular research. A current major goal of the field is to build on the past success of differentiation strategies to produce CMs with the properties of those originating from the different chambers of the adult human heart. With no anatomical origin or developmental pathway to draw on, the question of how to judge the success of such approaches and assess the chamber specificity of PSC-CMs has become increasingly important; commonly used methods have substantial limitations and are based on limited evidence to form such an assessment. In this article, we discuss the need for chamber-specific PSC-CMs in a number of areas as well as current approaches used to assess these cells on their likeness to those from different chambers of the heart. Furthermore, describing in detail the structural and functional features that distinguish the different chamber-specific human adult cardiac myocytes, we propose an evidence-based tool to aid investigators in the phenotypic characterization of differentiated PSC-CMs. Stem Cells 2017
Couch L, Derda AA, Thum T, et al., 2017, TAKOTSUBO SYNDROME ASSOCIATED MIR-16 AND MIR-26A REDUCE CONTRACTILITY OF CARDIOMYOCYTES IN VITRO BY AN INHIBITORY G-PROTEIN DEPENDENT MECHANISM, Annual Conference of the British-Cardiovascular-Society (BCS), Publisher: BMJ PUBLISHING GROUP, Pages: A135-A135, ISSN: 1355-6037
Watson S, Perbellini F, Harding SE, et al., 2017, Structural and functional plasticity of myocardial slices in response to prolonged mechanical loading and unloading in vitro, European Society of Cardiology Congress 2017
Mawad D, Mansfield C, Lauto A, et al., 2016, A conducting polymer with enhanced electronic stability applied in cardiac models, Science Advances, Vol: 2, ISSN: 2375-2548
Electrically active constructs can have a beneficial effect on electroresponsive tissues, such as the brain, heart, and nervous system. Conducting polymers (CPs) are being considered as components of these constructs because of their intrinsic electroactive and flexible nature. However, their clinical application has been largely hampered by their short operational time due to a decrease in their electronic properties. We show that, by immobilizing the dopant in the conductive scaffold, we can prevent its electric deterioration. We grew polyaniline (PANI) doped with phytic acid on the surface of a chitosan film. The strong chelation between phytic acid and chitosan led to a conductive patch with retained electroactivity, low surface resistivity (35.85 ± 9.40 kilohms per square), and oxidized form after 2 weeks of incubation in physiological medium. Ex vivo experiments revealed that the conductive nature of the patch has an immediate effect on the electrophysiology of the heart. Preliminary in vivo experiments showed that the conductive patch does not induce proarrhythmogenic activities in the heart. Our findings set the foundation for the design of electronically stable CP-based scaffolds. This provides a robust conductive system that could be used at the interface with electroresponsive tissue to better understand the interaction and effect of these materials on the electrophysiology of these tissues.
Kane C, Terracciano CM, 2016, Cardiac Fibroblast Co-Culture Promotes More Adult- Like Electrophysiological Properties in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes, Scientific Sessions of the American-Heart-Association / Resuscitation Science Symposium, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7322
Bello SO, Singh C, Jayakumar S, et al., 2016, Role of Cardiomyocyte Regeneration in Mechanical Unloading Induced Reverse Cardiac Remodeling, Scientific Sessions of the American-Heart-Association / Resuscitation Science Symposium, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7322
Watson SA, Perbellini F, Terracciano CM, 2016, Cardiac t-tubules: where structural plasticity meets functional adaptation., Cardiovascular Research, Vol: 112, Pages: 423-425, ISSN: 1755-3245
Bagdadi AV, Safari M, Dubey P, et al., 2016, Poly(3-hydroxyoctanoate), a promising new material for cardiac tissue engineering., Journal of Tissue Engineering and Regenerative Medicine, Vol: 12, Pages: e495-e512, ISSN: 1932-6254
Cardiac tissue engineering (CTE) is currently a prime focus of research due to an enormous clinical need. In this work, a novel functional material, Poly(3-hydroxyoctanoate), P(3HO), a medium chain length polyhydroxyalkanoate (PHA), produced using bacterial fermentation, was studied as a new potential material for CTE. Engineered constructs with improved mechanical properties, crucial for supporting the organ during new tissue regeneration, and enhanced surface topography, to allow efficient cell adhesion and proliferation, were fabricated. Our results showed that the mechanical properties of the final patches were close to that of cardiac muscle. Biocompatibility of the P(3HO) neat patches, assessed using Neonatal ventricular rat myocytes (NVRM), showed that the polymer was as good as collagen in terms of cell viability, proliferation and adhesion. Enhanced cell adhesion and proliferation properties were observed when porous and fibrous structures were incorporated to the patches. Also, no deleterious effect was observed on the adults cardiomyocytes' contraction when cardiomyocytes were seeded on the P(3HO) patches. Hence, P(3HO) based multifunctional cardiac patches are promising constructs for efficient CTE. This work will provide a positive impact on the development of P(3HO) and other PHAs as a novel new family of biodegradable functional materials with huge potential in a range of different biomedical applications, particularly CTE, leading to further interest and exploitation of these materials.
Poulet CE, Ramos S, Terracciano C, et al., 2016, Formation and stability of T-tubules in cardiomyocytes, Cardiovascular Research, Vol: 111, Pages: S17-S17, ISSN: 1755-3245
Perbellini F, Watson S, Scigliano M, et al., 2016, Cell therapy of the heart studied using adult myocardial slices in vitro, Cardiovascular Research, Vol: 111, Pages: S94-S94, ISSN: 1755-3245
Campagnolo P, Chiappini C, Leonardo V, et al., 2016, Porous silicon nanoneedles for localised in situ gene transfer for cardiac therapy, Cardiovascular Research, Vol: 111, Pages: S87-S87, ISSN: 1755-3245
Humphrey EJ, Mazo MM, Amdursky N, et al., 2016, Serum albumin hydrogels prevent de-differentiation of neonatal cardiomyocytes, Cardiovascular Research, Vol: 111, Pages: S80-S81, ISSN: 1755-3245
Mansfield C, Mawad D, Perbellini F, et al., 2016, A novel conductive patch for application in cardiac tissue engineering, Cardiovascular Research, Vol: 111, Pages: S41-S41, ISSN: 1755-3245
Scigliano M, Watson S, Tkach S, et al., 2016, Diastolic mechanical load delays structural and functional deterioration of ultrathin adult heart slices in culture, Cardiovascular Research, Vol: 111, Pages: S32-S32, ISSN: 1755-3245
Trantidou T, Humphrey EJ, Poulet C, et al., 2016, Surface chemistry and microtopography of Parylene C films control the morphology and microtubule density of cardiac myocytes., Tissue Engineering Part C-Methods, Vol: 22, ISSN: 1937-3392
Cell micro-patterning has certainly proved to improve the morphological and physiological properties of cardiomyocytes in vitro, however, there is little knowledge on the single cell-scaffold interactions that influence the cells' development and differentiation in culture. In this study, we employ hydrophobic/hydrophilic micro-patterned Parylene C thin films (2-10 µm) as cell micro-scaffolds that can control the morphology and microtubule density of neonatal rat ventricular myocytes (NRVM) by regulating their adhesion area on Parylene through a thickness-dependent hydrophobicity. Structured NRVM on thin films tend to bridge across the hydrophobic areas, demonstrating a more spread-out shape and sparser microtubule organization, while cells on thicker films adopt a cylindrical (in vivo-like) shape (contact angles at the level of the nucleus are 64.51<sup>o</sup> and 84.73<sup>o</sup> respectively) and a significantly (p <0.05) denser microtubule structure. Ion-scanning microscopy on NRVM revealed that cells on thicker membranes were significantly (p <0.05) smaller in volume but more elongated. The cylindrical shape and a significantly denser microtubule structure indicate the ability to influence cardiomyocyte phenotype using patterning and manipulation of hydrophilicity. These combined bioengineering strategies are promising tools in the generation of more representative cardiomyocytes in culture.
Kontziampasis D, Trantidou T, Regoutz A, et al., 2016, Effects of Ar and O<sub>2</sub> Plasma Etching on Parylene C: Topography versus Surface Chemistry and the Impact on Cell Viability, PLASMA PROCESSES AND POLYMERS, Vol: 13, Pages: 324-333, ISSN: 1612-8850
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- Citations: 23
Kane C, Du DTM, Hellen N, et al., 2016, The Fallacy of Assigning Chamber Specificity to iPSC Cardiac Myocytes from Action Potential Morphology, BIOPHYSICAL JOURNAL, Vol: 110, Pages: 281-283, ISSN: 0006-3495
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- Citations: 17
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