Imperial College London
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ProfessorCesareTerracciano

Faculty of Medicine, National Heart & Lung Institute

Professor of Cardiac Electrophysiology
 
 
 
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Contact

 

+44 (0)20 7594 2735c.terracciano Website CV

 
 
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Location

 

430ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pitoulis:2020:10.1016/j.yjmcc.2020.03.007,
author = {Pitoulis, FG and Hasan, W and Papadaki, M and Clavere, NG and Perbellini, F and Harding, SE and Kirk, JA and Boateng, SY and de, Tombe PP and Terracciano, CM},
doi = {10.1016/j.yjmcc.2020.03.007},
journal = {Journal of Molecular and Cellular Cardiology},
pages = {11--16},
title = {Intact myocardial preparations reveal intrinsic transmural heterogeneity in cardiac mechanics},
url = {http://dx.doi.org/10.1016/j.yjmcc.2020.03.007},
volume = {141},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Determining transmural mechanical properties in the heart provides a foundation to understand physiological and pathophysiological cardiac mechanics. Although work on mechanical characterisation has begun in isolated cells and permeabilised samples, the mechanical profile of living individual cardiac layers has not been examined. Myocardial slices are 300 μm-thin sections of heart tissue with preserved cellular stoichiometry, extracellular matrix, and structural architecture. This allows for cardiac mechanics assays in the context of an intact in vitro organotypic preparation. In slices obtained from the subendocardium, midmyocardium and subepicardium of rats, a distinct pattern in transmural contractility is found that is different from that observed in other models. Slices from the epicardium and midmyocardium had a higher active tension and passive tension than the endocardium upon stretch. Differences in total myocyte area coverage, and aspect ratio between layers underlined the functional readouts, while no differences were found in total sarcomeric protein and phosphoprotein between layers. Such intrinsic heterogeneity may orchestrate the normal pumping of the heart in the presence of transmural strain and sarcomere length gradients in the in vivo heart.
AU  - Pitoulis,FG
AU  - Hasan,W
AU  - Papadaki,M
AU  - Clavere,NG
AU  - Perbellini,F
AU  - Harding,SE
AU  - Kirk,JA
AU  - Boateng,SY
AU  - de,Tombe PP
AU  - Terracciano,CM
DO  - 10.1016/j.yjmcc.2020.03.007
EP  - 16
PY  - 2020///
SN  - 0022-2828
SP  - 11
TI  - Intact myocardial preparations reveal intrinsic transmural heterogeneity in cardiac mechanics
T2  - Journal of Molecular and Cellular Cardiology
UR  - http://dx.doi.org/10.1016/j.yjmcc.2020.03.007
UR  - https://www.sciencedirect.com/science/article/pii/S0022282820300705?via%3Dihub
VL  - 141
ER  -
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