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@article{Jabbour:2021:10.1172/jci.insight.144068,
author = {Jabbour, R and Owen, T and Pandey, P and reinsch, M and Wang, B and King, O and Couch, L and Pantou, D and Pitcher, D and Chowdhury, R and Pitoulis, F and Handa, B and Kit-Anan, W and Perbellini, F and myles, R and Stuckey, D and dunne, M and Shanmuganathan, M and Peters, N and Ng, FS and weinberger, F and Terracciano, C and smith, G and Eschenhagen, T and Harding, S},
doi = {10.1172/jci.insight.144068},
journal = {JCI Insight},
pages = {1--13},
title = {In vivo grafting of large engineered heart tissue patches for cardiac repair},
url = {http://dx.doi.org/10.1172/jci.insight.144068},
volume = {6},
year = {2021}
}

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TY  - JOUR
AB  - Engineered heart tissue (EHT) strategies, by combining cells within a hydrogel matrix, may be anovel therapy for heart failure. EHTs restore cardiac function in rodent injury models, but more dataare needed in clinically relevant settings. Accordingly, an upscaled EHT patch (2.5 cm × 1.5 cm × 1.5mm) consisting of up to 20 million human induced pluripotent stem cell–derived cardiomyocytes(hPSC-CMs) embedded in a fibrin-based hydrogel was developed. A rabbit myocardial infarctionmodel was then established to test for feasibility and efficacy. Our data showed that hPSC-CMs inEHTs became more aligned over 28 days and had improved contraction kinetics and faster calciumtransients. Blinded echocardiographic analysis revealed a significant improvement in function ininfarcted hearts that received EHTs, along with reduction in infarct scar size by 35%. Vascularizationfrom the host to the patch was observed at week 1 and stable to week 4, but electrical couplingbetween patch and host heart was not observed. In vivo telemetry recordings and ex vivoarrhythmia provocation protocols showed that the patch was not pro-arrhythmic. In summary, EHTsimproved function and reduced scar size without causing arrhythmia, which may be due to the lackof electrical coupling between patch and host heart.
AU  - Jabbour,R
AU  - Owen,T
AU  - Pandey,P
AU  - reinsch,M
AU  - Wang,B
AU  - King,O
AU  - Couch,L
AU  - Pantou,D
AU  - Pitcher,D
AU  - Chowdhury,R
AU  - Pitoulis,F
AU  - Handa,B
AU  - Kit-Anan,W
AU  - Perbellini,F
AU  - myles,R
AU  - Stuckey,D
AU  - dunne,M
AU  - Shanmuganathan,M
AU  - Peters,N
AU  - Ng,FS
AU  - weinberger,F
AU  - Terracciano,C
AU  - smith,G
AU  - Eschenhagen,T
AU  - Harding,S
DO  - 10.1172/jci.insight.144068
EP  - 13
PY  - 2021///
SN  - 2379-3708
SP  - 1
TI  - In vivo grafting of large engineered heart tissue patches for cardiac repair
T2  - JCI Insight
UR  - http://dx.doi.org/10.1172/jci.insight.144068
UR  - https://insight.jci.org/articles/view/144068
UR  - http://hdl.handle.net/10044/1/90648
VL  - 6
ER  -

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