Imperial College London
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ProfessorCesareTerracciano

Faculty of Medicine, National Heart & Lung Institute

Professor of Cardiac Electrophysiology
 
 
 
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Contact

 

+44 (0)20 7594 2735c.terracciano Website CV

 
 
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Location

 

430ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{King:2022:10.1016/j.crmeth.2022.100280,
author = {King, O and Cruz-Moreira, D and Sayed, A and Kermani, F and Kit-Anan, W and Sunyovszki, I and Wang, BX and Downing, B and Fourre, J and Hachim, D and Randi, AM and Stevens, MM and Rasponi, M and Terracciano, CM},
doi = {10.1016/j.crmeth.2022.100280},
journal = {Cell Reports: Methods},
pages = {1--16},
title = {Functional microvascularization of human myocardium in vitro},
url = {http://dx.doi.org/10.1016/j.crmeth.2022.100280},
volume = {2},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In this study, we report static and perfused models of human myocardial-microvascular interaction. In static culture, we observe distinct regulation of electrophysiology of human induced pluripotent stem cell derived-cardiomyocytes (hiPSC-CMs) in co-culture with human cardiac microvascular endothelial cells (hCMVECs) and human left ventricular fibroblasts (hLVFBs), including modification of beating rate, action potential, calcium handling, and pro-arrhythmic substrate. Within a heart-on-a-chip model, we subject this three-dimensional (3D) co-culture to microfluidic perfusion and vasculogenic growth factors to induce spontaneous assembly of perfusable myocardial microvasculature. Live imaging of red blood cells within myocardial microvasculature reveals pulsatile flow generated by beating hiPSC-CMs. This study therefore demonstrates a functionally vascularized in vitro model of human myocardium with widespread potential applications in basic and translational research.
AU  - King,O
AU  - Cruz-Moreira,D
AU  - Sayed,A
AU  - Kermani,F
AU  - Kit-Anan,W
AU  - Sunyovszki,I
AU  - Wang,BX
AU  - Downing,B
AU  - Fourre,J
AU  - Hachim,D
AU  - Randi,AM
AU  - Stevens,MM
AU  - Rasponi,M
AU  - Terracciano,CM
DO  - 10.1016/j.crmeth.2022.100280
EP  - 16
PY  - 2022///
SN  - 2667-2375
SP  - 1
TI  - Functional microvascularization of human myocardium in vitro
T2  - Cell Reports: Methods
UR  - http://dx.doi.org/10.1016/j.crmeth.2022.100280
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000907954800003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S2667237522001576?via%3Dihub
UR  - http://hdl.handle.net/10044/1/103021
VL  - 2
ER  -
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