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@article{Zhang:2018:10.4102/ajlm.v7i1.690,
author = {Zhang, A and Jumbe, E and Krysiak, R and Sidiki, S and Kelley, H and Chemey, EK and Kamba, C and Mwapasa, V and Garcia, J and Norris, A and Pan, XJ and Evans, C and Wang, S-H and Kwiek, JJ and Torrelles, JB},
doi = {10.4102/ajlm.v7i1.690},
journal = {African Journal of Laboratory Medicine},
title = {Low-cost diagnostic test for susceptible and drug-resistant tuberculosis in rural Malawi},
url = {http://dx.doi.org/10.4102/ajlm.v7i1.690},
volume = {7},
year = {2018}
}

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TY  - JOUR
AB  - Background: Rural settings where molecular tuberculosis diagnostics are not currently available need easy-to-use tests that do not require additional processing or equipment. While acid-fast bacilli (AFB) smear is the most common and often only tuberculosis diagnosis test performed in rural settings, it is labour intensive, has less-than-ideal sensitivity, and cannot assess tuberculosis drug susceptibility patterns.Objective: The objective of this study was to determine the feasibility of a multidrug-resistant (MDR) or extensively drug-resistant (XDR)-tuberculosis coloured agar-based culture test (tuberculosis CX-test), which can detect Mycobacterium tuberculosis growth and evaluate for drug susceptibility to isoniazid, rifampicin and a fluoroquinolone (i.e. ciprofloxacin) in approximately 14 days.Method: In this study, 101 participants were enrolled who presented to a rural health clinic in central Malawi. They were suspected of having active pulmonary tuberculosis. Participants provided demographic and clinical data and submitted sputum samples for tuberculosis testing using the AFB smear and tuberculosis CX-test.Results: The results showed a high level of concordance between the AFB smear (12 positive) and tuberculosis CX-test (13 positive); only one sample presented discordant results, with the molecular GeneXpert MTB/RIF® test confirming the tuberculosis CX-test results. The average time to a positive tuberculosis CX-test was 10 days. Of the positive samples, the tuberculosis CX-test detected no cases of drug resistance, which was later confirmed by the GeneXpert MTB/RIF®.Conclusion: These findings demonstrate that the tuberculosis CX-test could be a reliable low-cost diagnostic method for active pulmonary tuberculosis in high tuberculosis burden rural areas.
AU  - Zhang,A
AU  - Jumbe,E
AU  - Krysiak,R
AU  - Sidiki,S
AU  - Kelley,H
AU  - Chemey,EK
AU  - Kamba,C
AU  - Mwapasa,V
AU  - Garcia,J
AU  - Norris,A
AU  - Pan,XJ
AU  - Evans,C
AU  - Wang,S-H
AU  - Kwiek,JJ
AU  - Torrelles,JB
DO  - 10.4102/ajlm.v7i1.690
PY  - 2018///
SN  - 2225-2002
TI  - Low-cost diagnostic test for susceptible and drug-resistant tuberculosis in rural Malawi
T2  - African Journal of Laboratory Medicine
UR  - http://dx.doi.org/10.4102/ajlm.v7i1.690
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000433990100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/61342
VL  - 7
ER  -

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