Imperial College London
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Professor Carlton A W Evans

Faculty of Medicine, Department of Infectious Disease

Professor of Global Health
 
 
 
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Contact

 

+44 (0)20 3313 3222carlton.evans Website

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mekonnen:2019:10.1371/journal.pone.0215679,
author = {Mekonnen, B and Mihret, A and Getahun, M and Hailu, T and Sidiki, S and V, Kelley H and Scordo, JM and Hunt, WG and Pan, X and Balada-Llasat, J-M and Gebreyes, W and Evans, CA and Aseffa, A and Torrelles, JB and Wang, S-H and Abebe, T},
doi = {10.1371/journal.pone.0215679},
journal = {PLoS ONE},
title = {Evaluation of the tuberculosis culture color plate test for rapid detection of drug susceptible and drug-resistant Mycobacterium tuberculosis in a resource-limited setting, Addis Ababa, Ethiopia},
url = {http://dx.doi.org/10.1371/journal.pone.0215679},
volume = {14},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Timely diagnosis of tuberculosis (TB) is limited in Ethiopia. We evaluated the performance of a low technology, thin layer agar, Mycobacterium tuberculosis (M.tb) culture color plate (TB-CX) test with concurrent drug susceptibility testing (DST) to isoniazid (INH), rifampin (RIF), and pyrazinamide (PZA) directly from sputum specimens. Patients undergoing examination for TB and multidrug-resistant (MDR)-TB were enrolled in Addis Ababa, Ethiopia from March 2016 to February 2017. All subjects received a GeneXpert MTB/RIF PCR test. TB-CX test results were compared to reference Löwenstein-Jensen (LJ) culture for M.tb detection and DST for susceptibility to INH and RIF. Kappa statistic was applied to test agreement between results for TB-CX test and the reference methods, a cut-off Kappa value of 0.75 was considered as high level of agreements. A total of 137 participants were analyzed: 88 (64%) were new TB cases, 49 (36%) were re-treatment cases. The TB-CX test detected M.tb and DST in an average of 13 days compared to 50 days for the conventional DST result. The sensitivity and specificity of the TB-CX test for detecting M.tb were 94% and 98%, respectively (concordance, 96%; kappa 0.91). The sensitivity of the TB-CX test to detect drug resistance to INH, RIF, and MDR-TB was 91%, 100%, and 90% respectively. The specificity of the TB-CX test for detecting INH, RIF, and MDR-TB was 94%, 40%, and 94% respectively. Overall agreement between TB-CX test and LJ DST for detection of MDR-TB was 93%. The TB-CX test showed strong agreement with the GeneXpert test for detecting M.tb (89%, kappa 0.76) but low agreement for the detection of RIF resistance (57%, kappa 0.28). The TB-CX test was found to be a good alternative method for screening of TB and selective drug resistant-TB in a timely and cost-efficient manner.
AU  - Mekonnen,B
AU  - Mihret,A
AU  - Getahun,M
AU  - Hailu,T
AU  - Sidiki,S
AU  - V,Kelley H
AU  - Scordo,JM
AU  - Hunt,WG
AU  - Pan,X
AU  - Balada-Llasat,J-M
AU  - Gebreyes,W
AU  - Evans,CA
AU  - Aseffa,A
AU  - Torrelles,JB
AU  - Wang,S-H
AU  - Abebe,T
DO  - 10.1371/journal.pone.0215679
PY  - 2019///
SN  - 1932-6203
TI  - Evaluation of the tuberculosis culture color plate test for rapid detection of drug susceptible and drug-resistant Mycobacterium tuberculosis in a resource-limited setting, Addis Ababa, Ethiopia
T2  - PLoS ONE
UR  - http://dx.doi.org/10.1371/journal.pone.0215679
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31136575
UR  - http://hdl.handle.net/10044/1/70254
VL  - 14
ER  -
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