Imperial College London
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DrCarolinaHerrera

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

carolina.herrera

 
 
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Location

 

460 (Shattock Group)Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Thornhill:2019:10.1038/s41385-019-0180-2,
author = {Thornhill, J and Pace, M and Genevieve, M and Hoare, J and Peake, S and Herrera, C and Phetsouphanh, C and Meyerowitz, J and Hopkins, E and Brown, H and Dunn, P and Olejniczak, N and Wilberg, C and Klenerman, P and Goldin, R and Fox, J and Fidler, S and Frater, J},
doi = {10.1038/s41385-019-0180-2},
journal = {Mucosal Immunology},
pages = {1212--1219},
title = {CD32 expressing doublets in HIV infected gut-associated lymphoid are associated with a T follicular helper cell phenotype},
url = {http://dx.doi.org/10.1038/s41385-019-0180-2},
volume = {12},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Gut-associated lymphoid tissue (GALT) is a key location for the HIV reservoir. The observation that B-cell–T-cell doublets are enriched for CD32a (a low-affinity IgG receptor) in peripheral blood raises interesting questions, especially as these cells have been associated with HIV DNA in some studies. We sought to determine if similar doublets were present in GALT, the significance of these doublets, and their implications for the HIV reservoir. Given the importance of GALT as a reservoir for HIV, we looked for expression of CD32 on gut CD4 T cells and for evidence of doublets, and any relationship with HIV DNA in HIV + individuals initiated on antiretroviral therapy (ART) during primary HIV infection (PHI). Tonsil tissue was also available for one individual. As previously shown for blood, CD32high CD4 cells were mainly doublets of CD4 T cells and B cells, with T-cell expression of ICOS in tonsil and gut tissue. CD4 T cells associated with CD32 (compared with ‘CD32−' CD4 cells) had higher expression of follicular markers CXCR5, PD-1, ICOS, and Bcl-6 consistent with a T follicular helper (TFH) phenotype. There was a significant correlation between rectal HIV DNA levels and CD32 expression on TFH cells. Together, these data suggest that CD32high doublets are primarily composed of TFH cells, a subset known to be preferentially infected by HIV.
AU  - Thornhill,J
AU  - Pace,M
AU  - Genevieve,M
AU  - Hoare,J
AU  - Peake,S
AU  - Herrera,C
AU  - Phetsouphanh,C
AU  - Meyerowitz,J
AU  - Hopkins,E
AU  - Brown,H
AU  - Dunn,P
AU  - Olejniczak,N
AU  - Wilberg,C
AU  - Klenerman,P
AU  - Goldin,R
AU  - Fox,J
AU  - Fidler,S
AU  - Frater,J
DO  - 10.1038/s41385-019-0180-2
EP  - 1219
PY  - 2019///
SN  - 1933-0219
SP  - 1212
TI  - CD32 expressing doublets in HIV infected gut-associated lymphoid are associated with a T follicular helper cell phenotype
T2  - Mucosal Immunology
UR  - http://dx.doi.org/10.1038/s41385-019-0180-2
UR  - https://www.nature.com/articles/s41385-019-0180-2
UR  - http://hdl.handle.net/10044/1/70036
VL  - 12
ER  -
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