Imperial College London
Alternate

Dr Catriona M. McGilvery

Faculty of Engineering, Department of Materials

Research Facility Manager (Microscopy)
 
 
 
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Contact

 

+44 (0)20 7594 2579catriona.mcgilvery

 
 
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Location

 

LGM 05KRoyal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nitiputri:2016:10.1021/acsnano.6b02443,
author = {Nitiputri, K and Ramasse, QM and Autefage, H and McGilvery, CM and Boonrungsiman, S and Evans, ND and Stevens, MM and Porter, AE},
doi = {10.1021/acsnano.6b02443},
journal = {ACS Nano},
pages = {6826--6835},
title = {Nanoanalytical Electron Microscopy Reveals a Sequential Mineralization Process Involving Carbonate-Containing Amorphous Precursors},
url = {http://dx.doi.org/10.1021/acsnano.6b02443},
volume = {10},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A direct observation and an in-depth characterization of the steps by which bone mineral nucleates and grows in the extracellular matrix during the earliest stages of maturation, using relevant biomineralization models as they grow into mature bone mineral, is an important research goal. To better understand the process of bone mineralization in the extracellular matrix, we used nanoanalytical electron microscopy techniques to examine an in vitro model of bone formation. This study demonstrates the presence of three dominant CaP structures in the mineralizing osteoblast cultures: <80 nm dense granules with a low calcium to phosphate ratio (Ca/P) and crystalline domains; calcium phosphate needles emanating from a focus: “needle-like globules” (100–300 nm in diameter) and mature mineral, both with statistically higher Ca/P compared to that of the dense granules. Many of the submicron granules and globules were interspersed around fibrillar structures containing nitrogen, which are most likely the signature of the organic phase. With high spatial resolution electron energy loss spectroscopy (EELS) mapping, spatially resolved maps were acquired showing the distribution of carbonate within each mineral structure. The carbonate was located in the middle of the granules, which suggested the nucleation of the younger mineral starts with a carbonate-containing precursor and that this precursor may act as seed for growth into larger, submicron-sized, needle-like globules of hydroxyapatite with a different stoichiometry. Application of analytical electron microscopy has important implications in deciphering both how normal bone forms and in understanding pathological mineralization.
AU  - Nitiputri,K
AU  - Ramasse,QM
AU  - Autefage,H
AU  - McGilvery,CM
AU  - Boonrungsiman,S
AU  - Evans,ND
AU  - Stevens,MM
AU  - Porter,AE
DO  - 10.1021/acsnano.6b02443
EP  - 6835
PY  - 2016///
SN  - 1936-086X
SP  - 6826
TI  - Nanoanalytical Electron Microscopy Reveals a Sequential Mineralization Process Involving Carbonate-Containing Amorphous Precursors
T2  - ACS Nano
UR  - http://dx.doi.org/10.1021/acsnano.6b02443
UR  - http://hdl.handle.net/10044/1/36979
VL  - 10
ER  -
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