Imperial College London
Alternate

Prof. Chris Phillips

Faculty of Natural Sciences, Department of Physics

Professor of Experimental Solid State Physics
 
 
 
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Contact

 

+44 (0)20 7594 7575chris.phillips

 
 
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Assistant

 

Mrs Carolyn Dale +44 (0)20 7594 7579

 
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Location

 

911Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@inproceedings{Amrania:2015:10.1007/s00428-015-1805-9,
author = {Amrania, H and Shousha, S and Woodley, L and Goddard, K and Phillips, C and Coombes, C},
doi = {10.1007/s00428-015-1805-9},
pages = {S13--S13},
publisher = {Springer Verlag},
title = {Digistain: a novel biomarker imaging platform for grading breast carcinoma using routinely processed paraffin sections},
url = {http://dx.doi.org/10.1007/s00428-015-1805-9},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - CPAPER
AB  - Objective: Digistain is a new technology platform that enables imagingand quantification of a newly conceived biomarker for grading breastcarcinoma in routinely processed, unstained paraffin sections withoutthe use of traditional stains or contrasting agents. By recording a uniqueoptical signature to analyze the chemical make-up of a biopsy quantitatively, the technique is unaffected by the subjectivity of traditional grading. Within minutes of loading a slide it yields a highly reproducible and user independent numerical score reflecting the cellularity of the tumour and its nuclear: cytoplasmic ratio. We report here our findings using an objective technique to grade breast tumours using quantitative criteria.Method: H&E stained sections from excision biopsies of 105 cases ofinvasive breast carcinoma were reviewed and graded using the ElstonEllisgrading system. Unstained sections from each case were loaded intothe Digistain platform to yield a numerical score - the Digistain Index(DI).Results: The cases were grouped according to histological grading. MeanDIs was calculated for each grade (1,2 and 3) to be 0.56, 0.61, and 0.68respectively with a maximum standard error of 0.02. The DI spread withineach grade was less than that across the three grades, thus validatingthis index as a viable grading indicator within the context of this study.Conclusion: We believe the new Digistain approach provides for the firsttime a cost effective and quantitative measure of tumour grade. This canbe developed to deliver an effective assessment of prognosis and recurrence risk beyond traditional qualitative measures based on H&E staining protocols.
AU  - Amrania,H
AU  - Shousha,S
AU  - Woodley,L
AU  - Goddard,K
AU  - Phillips,C
AU  - Coombes,C
DO  - 10.1007/s00428-015-1805-9
EP  - 13
PB  - Springer Verlag
PY  - 2015///
SN  - 0945-6317
SP  - 13
TI  - Digistain: a novel biomarker imaging platform for grading breast carcinoma using routinely processed paraffin sections
UR  - http://dx.doi.org/10.1007/s00428-015-1805-9
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000359056400047&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/57771
ER  -
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