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@article{Mendes:2019:10.15252/msb.20198994,
author = {Mendes, ML and Fischer, L and Chen, ZA and Barbon, M and O'Reilly, FJ and Giese, SH and Bohlke-Schneider, M and Belsom, A and Dau, T and Combe, CW and Graham, M and Eisele, MR and Baumeister, W and Speck, C and Rappsilber, J},
doi = {10.15252/msb.20198994},
journal = {Mol Syst Biol},
title = {An integrated workflow for crosslinking mass spectrometry.},
url = {http://dx.doi.org/10.15252/msb.20198994},
volume = {15},
year = {2019}
}

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TY  - JOUR
AB  - We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and the crosslink identification software xiSEARCH. Sequential digestion enhances peptide detection by selective shortening of long tryptic peptides. We demonstrate our simple 12-fraction protocol for crosslinked multi-protein complexes and cell lysates, quantitative analysis, and high-density crosslinking, without requiring specific crosslinker features. This overall approach reveals dynamic protein-protein interaction sites, which are accessible, have fundamental functional relevance and are therefore ideally suited for the development of small molecule inhibitors.
AU  - Mendes,ML
AU  - Fischer,L
AU  - Chen,ZA
AU  - Barbon,M
AU  - O'Reilly,FJ
AU  - Giese,SH
AU  - Bohlke-Schneider,M
AU  - Belsom,A
AU  - Dau,T
AU  - Combe,CW
AU  - Graham,M
AU  - Eisele,MR
AU  - Baumeister,W
AU  - Speck,C
AU  - Rappsilber,J
DO  - 10.15252/msb.20198994
PY  - 2019///
TI  - An integrated workflow for crosslinking mass spectrometry.
T2  - Mol Syst Biol
UR  - http://dx.doi.org/10.15252/msb.20198994
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31556486
UR  - http://hdl.handle.net/10044/1/74010
VL  - 15
ER  -

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