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@article{Mendes:2019:10.1101/355396,
author = {Mendes, ML and Fischer, L and Chen, ZA and Barbon, M and OReilly, FJ and Giese, S and Bohlke-Schneider, M and Belsom, A and Dau, T and Combe, CW and Graham, M and Eisele, MR and Baumeister, W and Speck, C and Rappsilber, J},
doi = {10.1101/355396},
journal = {Molecular Systems Biology},
title = {An integrated workflow for crosslinking mass spectrometry},
url = {http://dx.doi.org/10.1101/355396},
year = {2019}
}

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TY  - JOUR
AB  - <jats:p>We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and the crosslink identification software Xi. Sequential digestion enhances peptide detection by selective shortening of long tryptic peptides. We demonstrate our simple 12-fraction protocol for crosslinked multi-protein complexes and cell lysates, quantitative analysis, and high-density crosslinking, without requiring specific crosslinker features. This overall approach reveals dynamic protein-protein interaction sites, which are accessible, have fundamental functional relevance and are therefore ideally suited for the development of small molecule inhibitors.</jats:p>
AU  - Mendes,ML
AU  - Fischer,L
AU  - Chen,ZA
AU  - Barbon,M
AU  - OReilly,FJ
AU  - Giese,S
AU  - Bohlke-Schneider,M
AU  - Belsom,A
AU  - Dau,T
AU  - Combe,CW
AU  - Graham,M
AU  - Eisele,MR
AU  - Baumeister,W
AU  - Speck,C
AU  - Rappsilber,J
DO  - 10.1101/355396
PY  - 2019///
SN  - 1744-4292
TI  - An integrated workflow for crosslinking mass spectrometry
T2  - Molecular Systems Biology
UR  - http://dx.doi.org/10.1101/355396
ER  -

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