Imperial College London
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ProfessorChristianSpeck

Faculty of Medicine, Institute of Clinical Sciences

Professor of Genome Biochemistry & Molecular Biology
 
 
 
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Contact

 

+44 (0)7961 815 557chris.speck Website CV

 
 
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Location

 

2.14BLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@inbook{Riera:2016:10.1007/978-3-319-24696-3_10,
author = {Riera, A and Speck, C},
booktitle = {The Initiation of DNA Replication in Eukaryotes},
doi = {10.1007/978-3-319-24696-3_10},
pages = {189--211},
title = {Licensing of replication origins},
url = {http://dx.doi.org/10.1007/978-3-319-24696-3_10},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - CHAP
AB  - All living organisms need to duplicate their genetic material prior to cell division in order to maintain genomic-stability. Cells have evolved sophisticated DNA replication mechanisms to ensure that this process is as faithful as possible. Eukaryotic initiation of DNA replication is a two-step process, where the replicative DNA helicase becomes loaded onto DNA to license DNA replication during late M-phase of the cell cycle prior to helicase-activation in S-phase. Importantly, helicase loading is entirely blocked in S-phase, which is a crucial regulatory mechanism that hinders re-replication of DNA and is crucial for genomic stability. Moreover, multiple copies of the replicative helicase become loaded at each origin to serve as backup-helicases in case a fork becomes terminally arrested. For these reasons it is imperative that helicase loading is as efficient as possible. MCM2-7 represent the core of the replicative helicase, which becomes loaded in an ATP-hydrolysis-dependent process as a double-hexamer onto double-stranded DNA. Current data suggest a model where ORC, Cdc6, and Cdt1 load in a stepwise process the MCM2-7 double-hexamer onto DNA. In this review we discuss the emerging mechanism of ATP-hydrolysis-driven helicase loading, the regulation of this process, and the structure and function of the MCM2-7 double-hexamer.
AU  - Riera,A
AU  - Speck,C
DO  - 10.1007/978-3-319-24696-3_10
EP  - 211
PY  - 2016///
SN  - 9783319246949
SP  - 189
TI  - Licensing of replication origins
T1  - The Initiation of DNA Replication in Eukaryotes
UR  - http://dx.doi.org/10.1007/978-3-319-24696-3_10
ER  -
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