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@misc{Hoyles:2017,
author = {Hoyles, L and Fernandez-Real, JM and Federici, M and Serino, M and Azalbert, V and Blasco, V and Abbott, J and Barton, RH and Puig, J and Xifra, G and Ricart, W and Woodbridge, M and Tomlinson, C and Cardelini, M and Davato, F and Cardolini, I and Porzio, O and Gentilieschi, P and Lopez, F and Foufelle, F and Postic, C and Butcher, SA and Holmes, E and Nicholson, JK and Burcenlin, R and Dumas, ME},
title = {Integrated systems biology to study non-alcoholic fatty liver disease in obese women},
type = {Poster},
year = {2017}
}

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TY  - GEN
AB  - Non-alcoholic fatty liver disease (NAFLD) is a multifactorial condition and one of the most common causes of chronic liver disease, with increasing worldwide prevalence. Microbiome-associated lipopolysaccharides (LPS) are associated with NAFLD in rodent models, but their relevance in human liver disease is not understood. In addition, microbiome-driven degradation of dietary choline – and its subsequent removal from host-associated metabolic processes – is thought to contribute to development of NAFLD. The FLORINASH study set out to determine the contribution of the gut microbiome to the NAFLD-associated molecular phenome (transcriptome, metabonome) independent of clinical confounders.Morbidly obese women [body mass index (BMI) >35] from Italy (n = 31) and Spain (n = 25) who elected for bariatric surgery were recruited to the study. Clinical data (28 variables) were recorded. Faecal samples, liver biopsies, blood and urine samples were collected. Faecal metagenomes were analysed using an in-house metagenomics pipeline (SCaleble Automated Metagenomics Pipeline). NAFLD activity score (NAS; 0, 1, 2, 3) was determined by histological examination of liver biopsies. Differentially expressed genes in hepatic transcriptomes were identified, and analysed using several complementary tools. 1H-NMR data were generated for plasma and urinary metabonomes. Clinical, metagenomic, transcriptomic and metabonomic data were integrated using partial Spearman’s correlation, taking identified confounders (age, BMI and cohort) into account.NAS was anti-correlated with microbial gene richness, and correlated with abundance of Gram-negative Proteobacteria. KEGG analyses of metagenomic data suggested increased microbial processing of dietary lipids and amino acids, as well as LPS-related processes associated with Proteobacteria in NAFLD. Activation of immune responses associated with Gram-negative (LPS-associated) microbial infections was correlated with NAS in hepatic tr
AU  - Hoyles,L
AU  - Fernandez-Real,JM
AU  - Federici,M
AU  - Serino,M
AU  - Azalbert,V
AU  - Blasco,V
AU  - Abbott,J
AU  - Barton,RH
AU  - Puig,J
AU  - Xifra,G
AU  - Ricart,W
AU  - Woodbridge,M
AU  - Tomlinson,C
AU  - Cardelini,M
AU  - Davato,F
AU  - Cardolini,I
AU  - Porzio,O
AU  - Gentilieschi,P
AU  - Lopez,F
AU  - Foufelle,F
AU  - Postic,C
AU  - Butcher,SA
AU  - Holmes,E
AU  - Nicholson,JK
AU  - Burcenlin,R
AU  - Dumas,ME
PY  - 2017///
TI  - Integrated systems biology to study non-alcoholic fatty liver disease in obese women
ER  -

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