Imperial College London

DrChrisWilson

Faculty of Natural SciencesDepartment of Life Sciences (Silwood Park)

Honorary Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2370chris.wilson Website

 
 
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Location

 

N3.11MunroSilwood Park

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Summary

 

Publications

Citation

BibTex format

@article{Wilson:2018:10.1016/j.cub.2018.05.070,
author = {Wilson, CG and Nowell, R and Barraclough, T},
doi = {10.1016/j.cub.2018.05.070},
journal = {Current Biology},
pages = {2436--2444.e14},
title = {Cross-contamination explains "inter- and intraspecific horizontal genetic transfers" between asexual bdelloid rotifers},
url = {http://dx.doi.org/10.1016/j.cub.2018.05.070},
volume = {28},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - A few metazoan lineages are thought to have persisted for millions of years without sexual reproduction. If so, they would offer important clues to the evolutionary paradox of sex itself [1, 2]. Most "ancient asexuals" are subject to ongoing doubt because extant populations continue to invest 17 in males [3–9]. However, males are famously unknown in bdelloid rotifers, a class of microscopic invertebrates comprising hundreds of species [10–12]. Bdelloid genomes have acquired an unusually high proportion of genes from non-metazoans via horizontal transfer [13–17]. This well-substantiated finding has invited speculation [13] that homologous horizontal transfer between bdelloid individuals also may occur, perhaps even "replacing" sex [14]. In 2016, Current Biology published an Article claiming to supply evidence for this idea. Debortoli et al. [18] sampled rotifers from natural populations and sequenced one mitochondrial and four nuclear loci. Species assignments were incongruent among loci for several samples, which was interpreted as evidence of "interspecific horizontal genetic transfers". Here, we use sequencing chromatograms supplied by the authors to demonstrate that samples treated as individuals actually contained two or more highly divergent mitoc hondrial and ribosomal sequences, revealing cross-contamination with DNA from multiple animals of different species. Other chromatograms indicate contamination with DNA from conspecific animals, explaining genetic and genomic evidence for "intraspecific horizontal exchanges" reported in the same study. Given the clear evidence of contamination, the data and findings of Debortoli et al. [18] provide no reliable support for their conclusions that DNA is transferred horizontally between or within bdelloid species.
AU - Wilson,CG
AU - Nowell,R
AU - Barraclough,T
DO - 10.1016/j.cub.2018.05.070
EP - 2444
PY - 2018///
SN - 1879-0445
SP - 2436
TI - Cross-contamination explains "inter- and intraspecific horizontal genetic transfers" between asexual bdelloid rotifers
T2 - Current Biology
UR - http://dx.doi.org/10.1016/j.cub.2018.05.070
UR - http://hdl.handle.net/10044/1/60272
VL - 28
ER -