234 results found
Dunsby C, French PMW, 2010, Biophotonics applications of supercontinuum generation, Supercontinuum Generation in Optical Fibers, Pages: 349-373, ISBN: 9780521514804
© Cambridge University Press 2010. Introduction Continua generated using high pulse energy laser systems to create broad spectra (Alfano and Shapiro, 1970) have been used for spectroscopy for many years (e.g. Busch et al., 1973). The application of fibre-generated continua to spectroscopy was suggested as early as 1976 in work by Lin and Stolen (1976) where a continuum spanning ∼450-600 nm was generated in a step-index fibre pumped by a nitrogen pumped dye laser. Since the demonstration of supercontinuum generation in microstructured optical fibres (MOF), however, the range of spectroscopic and imaging applications has increased enormously, owing to the high average powers, unprecedented spectral width and relatively low cost and low complexity of such sources. This chapter specifically focuses on the applications of supercontinua generated in MOFs and, in particular, on applications in biophotonics. MOF supercontinuum sources can be broadly grouped into three categories according to whether the laser pump source emits femtosecond pulses, picosecond-nanosecond pulses or cw radiation. In general terms, sub-ps pulses can produce broad supercontinua spanning from the UV to the NIR but the peak intensity damage threshold at the input end of the microstructured optical fibre limits the maximum average power that can be obtained in the supercontinuum to ∼<0.5 W with typically ∼<0.5 mW/nm available in the visible spectrum. The use of pump lasers with longer ps-ns pulses can significantly increase the maximum available average power before the onset of damage in the MOF such that high power supercontinua with several mW/nm in the visible can be achieved (e.g. Rulkov et al., 2005).
Kennedy GT, Manning HB, Elson DS, et al., 2010, A fluorescence lifetime imaging scanning confocal endomicroscope, JOURNAL OF BIOPHOTONICS, Vol: 3, Pages: 103-107, ISSN: 1864-063X
Robinson T, Manning HB, Dunsby C, et al., 2010, Investigating fast enzyme-DNA kinetics using multidimensional fluorescence imaging and microfluidics, Conference on Microfluidics, BioMEMS, and Medical Microsystems VIII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
McGinty J, Requejo-Isidro J, Munro I, et al., 2009, Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 42, ISSN: 0022-3727
Galletly N, McGinty J, Munro I, et al., 2009, Fluorescence lifetime imaging of liver cancer, 107th Annual Meeting of the American-Gastroenterlogical Association, Publisher: W B Saunders Co-Elsevier Inc
Schaerli Y, Wootton RC, Robinson T, et al., 2009, Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets, ANALYTICAL CHEMISTRY, Vol: 81, Pages: 302-306, ISSN: 0003-2700
Robinson T, Schaerli Y, Wootton R, et al., 2009, Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging, LAB ON A CHIP, Vol: 9, Pages: 3437-3441, ISSN: 1473-0197
Li R, Elson DS, Dunsby C, et al., 2009, A study on optical modulation signal and tissue displacement in Ultrasound Modulated Optical Tomography, Conference on Photons Plus Ultrasound - Imaging and Sensing 2009, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
McGinty J, Dunsby C, Auksorius E, et al., 2009, Multidimensional fluorescence imaging, Laboratory Techniques in Biochemistry and Molecular Biology (FLIM and FRET techniques), Editors: Gadella, Publisher: Elsevier, ISBN: 9780080915128
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips.
Dunsby C, 2008, Optically sectioned imaging by oblique plane microscopy, OPTICS EXPRESS, Vol: 16, Pages: 20306-20316, ISSN: 1094-4087
Talbot C, McGinty J, McGhee E, et al., 2008, High speed, optically sectioned fluorescence lifetime imaging utilizing time-gated nipkow disk or multifocal multiphoton time correlated single photon counting microscopy
We report two optically sectioned fluorescence lifetime systems that exhibit better signal to noise per unit time than conventional time correlated single photon counting systems. Both systems are applied to biologically relevant samples. ©2007 Optical Society of America.
Manning HB, Kennedy GT, Owen DM, et al., 2008, A compact, multidimensional spectrofluorometer exploiting supercontinuum generation, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 494-505, ISSN: 1864-063X
Talbot CB, McGinty J, Grant DM, et al., 2008, High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 514-521, ISSN: 1864-063X
McGinty J, Tahir KB, Soloviev VY, et al., 2008, Time-domain fluorescence lifetime tomography
We present a platform for fluorescence lifetime tomography utilising tuneable supercontinuum excitation and wide-field time-gated technology. Applied to optical projection and diffuse fluorescence tomography, we demonstrate 3D time-resolved fluorescence reconstruction in transparent and scattering phantoms.
Grant DM, Zhang W, McGhee EJ, et al., 2008, Multiplexed FRET to Image Multiple Signaling Events in Live Cells, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L69-L71, ISSN: 0006-3495
Oddos S, Dunsby C, Purbhoo MA, et al., 2008, High-Speed High-Resolution Imaging of Intercellular Immune Synapses Using Optical Tweezers, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L66-L68, ISSN: 0006-3495
McGinty J, Tahir KR, Laine R, et al., 2008, Fluorescence lifetime optical projection tomography, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 390-394, ISSN: 1864-063X
Robinson T, Valluri P, Manning HB, et al., 2008, Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 1887-1889, ISSN: 0146-9592
Galletly NP, McGinty J, Dunsby C, et al., 2008, Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin, BRITISH JOURNAL OF DERMATOLOGY, Vol: 159, Pages: 152-161, ISSN: 0007-0963
Auksorius E, Boruah BR, Dunsby C, et al., 2008, Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 113-115, ISSN: 0146-9592
De Beule PAA, Dunsby C, Galletly NP, et al., 2007, A hyperspectral fluorescence lifetime probe for skin cancer diagnosis, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 78, ISSN: 0034-6748
Owen DM, Auksorius E, Manning HB, et al., 2007, Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source, OPTICS LETTERS, Vol: 32, Pages: 3408-3410, ISSN: 0146-9592
Grant DM, McGinty J, McGhee EJ, et al., 2007, High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events, Optics Express, Vol: 15, Pages: 16656-16673
We present a time domain optically sectioned fluorescence lifetime imaging (FLIM) microscope developed for high-speed live cell imaging. This single photon excited system combines wide field parallel pixel detection with confocal sectioning utilizing spinning Nipkow disc microscopy. It can acquire fluorescence lifetime images of live cells at up to 10 frames per second (fps), permitting high-speed FLIM of cell dynamics and protein interactions with potential for high throughput cell imaging and screening applications. We demonstrate the application of this FLIM microscope to real-time monitoring of changes in lipid order in cell membranes following cholesterol depletion using cyclodextrin and to the activation of the small GTP-ase Ras in live cells using FRET. © 2007 Optical Society of America.
Manning HB, Owen DM, Auksorius E, et al., 2007, Applications of rapid time-gated hyperspectral FLIM: Live cell imaging of membrane order and 6-D microscopy, ISSN: 1605-7422
We describe the characterisation of a hyperspectral fluorescence lifetime imaging microscope that exploits high-speed time-gated imaging technology and a tunable continuum source for 6-D fluorescence imaging. This line-scanning confocal microscope can record the full spectral-temporal (i.e. excitation-emission-lifetime) fluorescence matrix at each pixel in a three dimensional (x-y-z) sample. This instrument has been applied to biological samples including model membranes and live cells labelled with the phase-sensitive membrane dye di-4-ANEPPDHQ, for which significant variation of lifetime with emission wavelength is observed. © 2007 SPIE-OSA.
Grant DM, McGinty J, McGhee EJ, et al., 2007, High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events, OPTICS EXPRESS, Vol: 15, Pages: 15656-15673, ISSN: 1094-4087
Benninger RK, Hofmann O, Onfelt B, et al., 2007, Fluorescence-Lifetime Imaging of DNA-Dye Interactions within Continuous-Flow Microfluidic Systems., Angew Chem Int Ed Engl, Vol: 46, Pages: 8536-8536, ISSN: 1433-7851
Kumar S, Dunsby C, De Beule PAA, et al., 2007, Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging, Optics Express, Vol: 15, Pages: 12548-12561
We report a multifocal multiphoton time-correlated single photon counting (TCSPC) fluorescence lifetime imaging (FLIM) microscope system that uses a 16 channel multi-anode PMT detector. Multiphoton excitation minimizes out-of-focus photobleaching, multifocal excitation reduces non-linear in-plane photobleaching effects and TCSPC electronics provide photon-efficient detection of the fluorescence decay profile. TCSPC detection is less prone to bleaching- and movementinduced artefacts compared to wide-field time-gated or frequency-domain FLIM. This microscope is therefore capable of acquiring 3-D FLIM images at significantly increased speeds compared to single beam multiphoton microscopy and we demonstrate this with live cells expressing a GFP tagged protein. We also apply this system to time-lapse FLIM of NAD(P)H autofluorescence in single live cells and report measurements on the change in the fluorescence decay profile following the application of a known metabolic inhibitor.
Garcia DI, Lanigan P, Webb M, et al., 2007, Fluorescence lifetime imaging to detect actomyosin states in mammalian muscle sarcomeres, BIOPHYSICAL JOURNAL, Vol: 93, Pages: 2091-2101, ISSN: 0006-3495
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