Imperial College London

Dr Chris Dunsby

Faculty of Natural SciencesDepartment of Physics

Professor of Biomedical Optics
 
 
 
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Contact

 

+44 (0)20 7594 7755christopher.dunsby Website

 
 
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Location

 

622Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

235 results found

Hegyi L, Talbot C, Monaco C, Sandison A, Davies AH, Peston D, Lever MJ, French PM, Stamp GWet al., 2007, Fluorescence lifetime imaging of unstained human atherosclerotic plaques., The 14th International Symposium on Atherosclerosis

Conference paper

Owen DM, Manning HB, de Beule P, Talbot C, Requejo-Isidro J, Dunsby C, McGinty J, Benninger RKP, Elson DS, Munro I, Galletly NP, Lever MJ, Stamp GW, Anand P, Neil MAA, French PMWet al., 2007, Development of a hyperspectral fluorescence lifetime imaging microscope and its application to tissue imaging, Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

Conference paper

Manning HB, Owen DM, Auksorius E, de Beule PAA, Oddos S, Talbot CB, Dunsby C, Munro I, Magee AI, Neil MAA, French PMWet al., 2007, Applications of rapid time-gated hyperspectral FLIM: live cell imaging of membrane order and 6-D microscopy, Conference on Confocal, Multiphoton and Nonlinear Microscopic Imaging III, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

Conference paper

Benninger RKP, Hofmann O, Onfelt B, Munro I, Dunsby C, Davis DM, Neil MAA, French PMW, de Mello AJet al., 2007, Fluorescence-lifetime imaging of DNA-Dye interactions within continuous-flow microfluidic systems (vol 46, pg 8525, 2007), ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 46, Pages: 8536-8536, ISSN: 1433-7851

Journal article

Benninger RKP, Hofmann O, Onfelt B, Munro I, Dunsby C, Davis DM, Neil MAA, French PMW, de Mello AJet al., 2007, Fluorescence-lifetime imaging of DNA-dye interactions within continuous-flow microfluidic systems, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 46, Pages: 2228-2231, ISSN: 1433-7851

Journal article

Owen D M, Manning H B, de Beule P, Talbot C B, Requejo-Isidro J, Dunsby C W, McGinty J A, Benninger, R K P, Elson D S, Munro I H, Galletly N P, Lever J, Stamp G W, Anand P, Neil, M A A, French, P M Wet al., 2007, Rapid hyperspectral fluorescence lifetime imaging, IMAGING, MANIPULATION AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES V, BIOS, Publisher: SPIE

Conference paper

De Beule PAA, Dunsby C, Owen DM, Galletly NP, Anand U, Benham CD, Naylor A, Stamp GW, Anand P, French PMWet al., 2007, A novel hyperspectral lifetime probe for autofluorescence - art. no. 643303, Conference on Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, Pages: 43303-43303

The application of autofluorescence in non-invasive medical diagnostics could have great potential. Two major drawbacks inherent to this approach are low signal levels compared to those from exogenous fluorescent probes and complexity caused by the multiplicity of fluorescent biomolecules in tissue. Here we present a new optical system that is based on single channel detection via all optical fiber and call measure the fluorescence emission spectrum and fluorescence lifetime simultaneously for excitation wavelengths of 355 and 435nm. Single channel measurements integrate the signal normally available in all imaging setup and therefore have a better signal-to-noise ratio. Resolving both the fluorescence emission spectrum and fluorescence lifetime provides the opportunity to discriminate multiple fluorophores. This instrument is intended for NAD(P)H and flavin measurements for the dynamic monitoring of cellular metabolism and optical measurements of cancerous tissue. Initial results from a study of live cells and a clinical study of human skin lesions are presented.

Conference paper

Treanor B, Lanigan PMP, Kumar S, Dunsby C, Munro I, Auksorius E, Culley FJ, Purbhoo MA, Phillips D, Neil MAA, Burshtyn DN, French PMW, Davis DMet al., 2006, Microclusters of inhibitory killer immunoglobulin like receptor signaling at natural killer cell immunological synapses, Journal of Cell Biology, Vol: 174, Pages: 153-161, ISSN: 1540-8140

We report the supramolecular organization of killer Ig–like receptor (KIR) phosphorylation using a technique applicable to imaging phosphorylation of any green fluorescent protein–tagged receptor at an intercellular contact or immune synapse. Specifically, we use fluorescence lifetime imaging (FLIM) to report Förster resonance energy transfer (FRET) between GFP-tagged KIR2DL1 and a Cy3-tagged generic anti-phosphotyrosine monoclonal antibody. Visualization of KIR phosphorylation in natural killer (NK) cells contacting target cells expressing cognate major histocompatibility complex class I proteins revealed that inhibitory signaling is spatially restricted to the immune synapse. This explains how NK cells respond appropriately when simultaneously surveying susceptible and resistant target cells. More surprising, phosphorylated KIR was confined to microclusters within the aggregate of KIR, contrary to an expected homogeneous distribution of KIR signaling across the immune synapse. Also, yellow fluorescent protein–tagged Lck, a kinase important for KIR phosphorylation, accumulated in a multifocal distribution at inhibitory synapses. Spatial confinement of receptor phosphorylation within the immune synapse may be critical to how activating and inhibitory signals are integrated in NK cells.

Journal article

Owen DM, Lanigan PMP, Dunsby C, Munro I, Grant D, Neil MAA, French PMW, Magee AIet al., 2006, Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells, BIOPHYSICAL JOURNAL, Vol: 90, Pages: L80-L82, ISSN: 0006-3495

Journal article

Galletly N, Mcginty JM, Cohen P, Munro I, Elson DS, Requejo-Isidro J, Dunsby C, Neil MA, Thillainayagam AV, French PM, Stamp GWet al., 2006, Fluorescence Lifetime Imaging for Detection of Gastrointestinal Cancer, GASTROINTESTINAL ENDOSCOPY, Vol: 63, Pages: AB250-AB250, ISSN: 0016-5107

Journal article

Galletly NP, McGinty JM, Cohen P, Munro I, Elson DS, Requejo-Isidro J, Dunsby C, Neil MAA, Thillainayagam AV, French PMW, Stamp GWet al., 2006, Detection of gastrointestinal malignancy by fluorescence lifetime imaging of UV laser induced tissue autofluorescence, Annual Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A115-A115, ISSN: 0017-5749

Conference paper

Galletly N, McGinty J, Munro I, Elson DS, Requejo-Isidro J, Dunsby C, Neil MA, Thillainayagam AV, French PM, Stamp GWet al., 2006, Fluorescence lifetime imaging of liver cancer, Digestive Disease Week Meeting/107th Annual Meeting of the American-Gastroenterlogical-Association, Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: A791-A791, ISSN: 0016-5085

Conference paper

Lanigan PMP, Dunsby C, McGinty J, Elson DS, Requejo-Isidro J, Munro I, Galletly N, Treanor B, Önfelt B, McCann F, Davis DM, Neil MAA, French PMWet al., 2006, An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy

We demonstrate that spectral selection from a supercontinuum generated in a microstructured fibre can provide a continuously electronically tunable ultrafast spatially coherent source for confocal microscopy and both scanning and wide field fluorescence lifetime imaging. © 2005 Optical Society of America.

Conference paper

Lekadir, Karim, Elson, Daniel S, Requejo-Isidro, Jose, Dunsby, Christopher, McGinty, James, Galletly, Neil, Stamp, Gordon, French, Paul MW, Yang, Guang-Zhonget al., 2006, Tissue Characterization using Dimensionality Reduction and Fluorescence Imaging, Medical Image Computing and Computer Assisted Intervention, Publisher: Medical Image Computing and Computer Assisted Intervention

Conference paper

Lekadir K, Elson DS, Requejo-Isidro J, Dunsby C, McGinty J, Galletly N, Stamp G, French PMW, Yang G-Zet al., 2006, Tissue characterization using dimensionality reduction and fluorescence imaging, MEDICAL IMAGE COMPUTING AND COMPUTER-ASSISTED INTERVENTION - MICCAI 2006, PT 2, Vol: 4191, Pages: 586-593, ISSN: 0302-9743

Journal article

Grant DM, Elson DS, Schimpf D, Dunsby C, Requejo-Isidro J, Auksorius E, Munro I, Neil MAA, French PMW, Nye E, Stamp G, Courtney Pet al., 2005, Optically sectioned fluorescence lifetime imaging using a Nipkow disk microscope and a tunable ultrafast continuum excitation source, OPTICS LETTERS, Vol: 30, Pages: 3353-3355, ISSN: 0146-9592

Journal article

Munro I, McGinty J, Galletly N, Requejo-Isidro J, Lanigan PMP, Elson DS, Dunsby C, Neil MAA, Lever MJ, Stamp GWH, French PMWet al., 2005, Toward the clinical application of time-domain fluorescence lifetime imaging, JOURNAL OF BIOMEDICAL OPTICS, Vol: 10, ISSN: 1083-3668

Journal article

Benninger RKP, McGinty J, Hofmann O, Requejo-Isidro J, Munro I, Elson DS, Dunsby CW, Onfelt B, Davis DM, Neil MAA, de Mello AJ, French PMWet al., 2005, Application of multi-dimensional fluorescence imaging to microfluidic devices, European Conference on Lasers and Electro-Optics

Conference paper

Lanigan PMP, Dunsby C, McGinty J, Elson DS, Requejo-Isidro J, Munro I, Galletly N, Treanor B, Önfelt B, McCann F, Davis DM, Neil MAA, French PMWet al., 2005, An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy, Pages: 1366-1368

We demonstrate that spectral selection from a supercontinuum generated in a microstructured fibre can provide a continuously electronically tunable ultrafast spatially coherent source for confocal microscopy and both scanning and wide field fluorescence lifetime imaging. © 2005 Optical Society of America.

Conference paper

Dunsby C, Lanigan PMP, McGinty J, De Beule PAA, Auksorius E, Schimpf DN, Elson DS, Requejo-Isidro J, Munro I, Galletly N, Treanor B, Önfelt B, McCann F, Davis DM, Neil MAA, French PMWet al., 2005, An electronically tunable ultrafast laser source applied to fluorescence imaging and microscopy including fluorescence lifetime imaging

Conference paper

Talbot CB, Benninger RKP, De Beule P, Requejo-Isidro J, Elson DS, Dunsby C, Munro I, Neil MA, Sandison A, Sofat N, Nagase H, French PMW, Lever MJet al., 2005, Application of fluorescence lifetime and hyperspectral imaging to tissue autofluorescence: Arthritis

We present the application of fluorescence lifetime imaging (FLIM) and hyperspectral FLIM to autofluorescence of normal and arthritic cartilage tissue. ©2005 Optical Society of America.

Conference paper

Lanigan PMP, Dunsby C, McGinty J, Elson DS, Requejo-Isidro J, Munro I, Galletly N, Treanor B, Onfelt B, McCann F, Davis DM, Neil MAA, French PMWet al., 2005, An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy, Washington, CONFERENCE ON LASERS & ELECTRO-OPTICS (CLEO), Publisher: Optical Society of America, Pages: 1366-1368

Conference paper

McGinty J, Munro I, Requejo-Isidro J, Elson DS, Dunsby C, Neil MAA, French PMW, Galletly NP, Stamp GWHet al., 2005, Video rate fluorescence lifetime imaging and fluorescence lifetime endoscopy (Article no.586408), Proceedings- Spie the International Society for Optical Engineering, Vol: 5864, ISSN: 0277-786X

Journal article

McGinty J, Munro I, Requejo-Isidro J, Elson DS, Dunsby C, Neil MAA, French PMW, Galletly NP, Stamp GWHet al., 2005, Video rate fluorescence lifetime imaging and fluorescence lifetime endoscopy (Article no.586408), ISSN: 0277-786X

Conference paper

Grant D, Auksorius E, Schimpf D, Elson DS, Dunsby C, Requejo-Isidro J, Munro I, Neil M, French PMW, Courtney Pet al., 2005, Fluorescence lifetime imaging microscopy using a tunable continuum source and a Nipkow disk confocal microscope (Article no.58600K), ISSN: 0277-786X

Conference paper

Grant D, Auksorius E, Schimpf D, Elson DS, Dunsby C, Requejo-Isidro J, Munro I, Neil M, French PMW, Courtney Pet al., 2005, Fluorescence lifetime imaging microscopy using a tunable continuum source and a Nipkow disk confocal microscope (Article no.58600K), Proceedings- Spie the International Society for Optical Engineering, Vol: 5860, ISSN: 0277-786X

Journal article

Talbot CB, Benninger RKP, de Beule P, Requejo-Isidro J, Elson DS, Dunsby C, Munro I, Neil MA, Sandison A, Sofat Net al., 2005, Application of hyperspectral fluorescence lifetime imaging to tissue autofluorescence: arthritis (Article no.58620T), Proceedings- Spie the International Society for Optical Engineering, Vol: 5862, ISSN: 0277-786X

Journal article

Grant D, Auksorius E, Schimpf D, Elson DS, Dunsby C, Requejo-Isidro J, Munro I, Neil M, French PMW, Courtney Pet al., 2005, Fluorescence lifetime imaging microscopy using a tunable continuum source and a Nipkow disk confocal microscope (Article no.58600K), Proceedings- Spie the International Society for Optical Engineering, Vol: 5860, ISSN: 0277-786X

Journal article

French PMW, de Beule P, Requejo-Isidro J, Dunsby C, Lanigan P, McGinty J, Talbot CB, Elson DS, Munro I, Galletly N, Neil MAAet al., 2005, Multidimensional fluorescence imaging, GIT Imaging and Microscopy, Vol: 3, Pages: 20-22, ISSN: 1439-4243

Journal article

McGinty J, Munro I, Requejo-Isidro J, Elson DS, Dunsby C, Neil MAA, French PMW, Galletly NP, Stamp GWHet al., 2005, Video rate fluorescence lifetime imaging and fluorescence lifetime endoscopy (Article no.586408), Proceedings- Spie the International Society for Optical Engineering, Vol: 5864, ISSN: 0277-786X

Journal article

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