Imperial College London

Dr Chris Dunsby

Faculty of Natural SciencesDepartment of Physics

Professor of Biomedical Optics
 
 
 
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Contact

 

+44 (0)20 7594 7755christopher.dunsby Website

 
 
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Location

 

622Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lagarto:2019:10.1364/BOE.10.003506,
author = {Lagarto, J and Dyer, B and Dunsby, C and Peters, N and French, P and Dunsby, C and Lyon, A},
doi = {10.1364/BOE.10.003506},
journal = {Biomedical Optics Express},
pages = {3506--3521},
title = {In vivo label-free optical monitoring of structural and metabolic remodeling of myocardium following infarction},
url = {http://dx.doi.org/10.1364/BOE.10.003506},
volume = {10},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Cardiac remodeling following myocardial infarction (MI) involves structural and functional alterations in the infarcted and remote viable myocardium that can ultimately lead to heart failure. The underlying mechanisms are not fully understood and, following our previous study of the autofluorescence lifetime and diffuse reflectance signatures of the myocardium in vivo at 16 weeks post MI in rats [Biomed. Opt. Express 6(2), 324 (2015)], we here present data obtained at 1, 2 and 4 weeks post myocardial infarction that help follow the temporal progression of these changes. Our results demonstrate that both structural and metabolic changes in the heart can be monitored from the earliest time points following MI using label-free optical readouts, not only in the region of infarction but also in the remote non-infarcted myocardium. Changes in the autofluorescence intensity and lifetime parameters associated with collagen type I autofluorescence were indicative of progressive collagen deposition in tissue that was most pronounced at earlier time points and in the region of infarction. In addition to significant collagen deposition in infarcted and non-infarcted myocardium, we also report changes in the autofluorescence parameters associated with reduced nicotinamide adenine (phosphate) dinucleotide (NAD(P)H) and flavin adenine dinucleotide (FAD), which we associate with metabolic alterations throughout the heart. Parallel measurements of the diffuse reflectance spectra indicated an increased contribution of reduced cytochrome c. Our findings suggest that combining time-resolved spectrofluorometry and diffuse reflectance spectroscopy could provide a useful means to monitor cardiac function in vivo at the time of surgery.
AU - Lagarto,J
AU - Dyer,B
AU - Dunsby,C
AU - Peters,N
AU - French,P
AU - Dunsby,C
AU - Lyon,A
DO - 10.1364/BOE.10.003506
EP - 3521
PY - 2019///
SN - 2156-7085
SP - 3506
TI - In vivo label-free optical monitoring of structural and metabolic remodeling of myocardium following infarction
T2 - Biomedical Optics Express
UR - http://dx.doi.org/10.1364/BOE.10.003506
UR - https://www.osapublishing.org/boe/abstract.cfm?uri=boe-10-7-3506
UR - http://hdl.handle.net/10044/1/70738
VL - 10
ER -