Imperial College London

Dr Chris Dunsby

Faculty of Natural SciencesDepartment of Physics

Reader in Biomedical Optics
 
 
 
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Contact

 

+44 (0)20 7594 7755christopher.dunsby Website

 
 
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Location

 

622Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@inproceedings{Görlitz:2020:10.1117/12.2550682,
author = {Görlitz, F and Wysoczanski, R and Kumar, S and Lightley, J and Garcia, E and Alexandrov, Y and Munro, I and Johnson, S and Kehoe, M and Hollick, C and Graham, J and Donnelly, L and Barnes, P and Dunsby, C and Neil, MAA and French, PMW},
doi = {10.1117/12.2550682},
title = {Towards easier, faster, super-resolved microscopy},
url = {http://dx.doi.org/10.1117/12.2550682},
year = {2020}
}

RIS format (EndNote, RefMan)

TY  - CPAPER
AB - © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. Super-resolved microscopy techniques have overcome the diffraction limit to provide image resolutions approaching the scale of fluorescent labels. However, many of these techniques require significant experimental resources and expertise and impose long image data acquisition times, making it difficult to acquire super-resolved data from sufficiently large sample numbers to overcome intrinsic biological variation. We have worked to make stimulated emission depletion (STED) microscopy and single molecule localisation microscopy (SMLM) more straightforward to implement and more practical to image larger numbers of cells. Here we present work in progress developing easySLM STED and easySTORM, including a new modular microscope frame that we believe can make it easier to prototype microscopy techniques and to implement and maintain them in lower resourced settings.
AU - Görlitz,F
AU - Wysoczanski,R
AU - Kumar,S
AU - Lightley,J
AU - Garcia,E
AU - Alexandrov,Y
AU - Munro,I
AU - Johnson,S
AU - Kehoe,M
AU - Hollick,C
AU - Graham,J
AU - Donnelly,L
AU - Barnes,P
AU - Dunsby,C
AU - Neil,MAA
AU - French,PMW
DO - 10.1117/12.2550682
PY - 2020///
SN - 1605-7422
TI - Towards easier, faster, super-resolved microscopy
UR - http://dx.doi.org/10.1117/12.2550682
ER -