Imperial College London

Dr Chris Dunsby

Faculty of Natural SciencesDepartment of Physics

Reader in Biomedical Optics
 
 
 
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Contact

 

+44 (0)20 7594 7755christopher.dunsby Website

 
 
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Location

 

622Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@inproceedings{Garcia:2020:10.1117/12.2547517,
author = {Garcia, E and Guo, W and Kumar, S and Görlitz, F and Sparks, H and Alexandrov, Y and Munro, I and Kelly, DJ and Warren, S and Chennell, G and Sardini, A and Carling, D and Thorpe, P and Dunsby, C and French, PMW},
doi = {10.1117/12.2547517},
title = {FLIM, FRET and high content analysis},
url = {http://dx.doi.org/10.1117/12.2547517},
year = {2020}
}

RIS format (EndNote, RefMan)

TY  - CPAPER
AB - © 2020 SPIE. Fluorescence lifetime imaging (FLIM) provides a means to contrast different molecular species and to map variations in the local fluorophore molecular environment, including to read out Förster resonant energy transfer (FRET), e.g.To assay protein interactions or genetically expressed FRET biosensors. We have implemented wide-field time-gated FLIM in a modular open automated microscopy platform for high content analysis (HCA). To demonstrate its relevance to drug discovery, we have demonstrated the capability of our openFLIM HCA platform to assay interactions of low copy number endogenous proteins in yeast cells labelled with fluorescent proteins. We have also demonstrated the capability of multiwell plate FLIM assays to provide readouts of a FRET biosensor in 2-D and 3-D cell cultures.
AU - Garcia,E
AU - Guo,W
AU - Kumar,S
AU - Görlitz,F
AU - Sparks,H
AU - Alexandrov,Y
AU - Munro,I
AU - Kelly,DJ
AU - Warren,S
AU - Chennell,G
AU - Sardini,A
AU - Carling,D
AU - Thorpe,P
AU - Dunsby,C
AU - French,PMW
DO - 10.1117/12.2547517
PY - 2020///
SN - 1605-7422
TI - FLIM, FRET and high content analysis
UR - http://dx.doi.org/10.1117/12.2547517
ER -