Publications
273 results found
Sonnefraud Y, Sinclair HG, Sivan Y, et al., 2014, Experimental Proof of Concept of Nanoparticle-Assisted STED, NANO LETTERS, Vol: 14, Pages: 4449-4453, ISSN: 1530-6984
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- Citations: 26
Dunsby C, Mcginty J, French P, 2014, Multidimensional fluorescence imaging of biological tissue., Biomedical Photonics Handbook, Pages: 531-560, ISBN: 9781439804445
Dyer B, Lagarto J, Sikkel M, et al., 2014, THE APPLICATION OF AUTOFLUORESCENCE LIFETIME METROLOGY AS A NOVEL LABEL-FREE TECHNIQUE FOR THE ASSESSMENT OF CARDIAC DISEASE, HEART, Vol: 100, Pages: A104-A104, ISSN: 1355-6037
Gore DM, Margineanu A, French P, et al., 2014, Two-photon fluorescence (TPF) microscopy of corneal riboflavin absorption, Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Gore DM, Margineanu A, French P, et al., 2014, Two-Photon Fluorescence Microscopy of Corneal Riboflavin Absorption, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol: 55, Pages: 2476-2481, ISSN: 0146-0404
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- Citations: 27
sparks H, warren S, Guedes J, et al., 2014, A flexible wide-field FLIM endoscope utilising blue excitation light for label-free contrast of tissue., Journal of Biophotonics, Vol: 8, Pages: 168-178, ISSN: 1864-0648
Coda S, Thompson AJ, Kennedy GT, et al., 2014, Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe, Biomedical Optics Express, Vol: 5, Pages: 515-538, ISSN: 2156-7085
We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime −570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens.
Lenz MO, Sinclair HG, Savell A, et al., 2014, 3-D stimulated emission depletion microscopy with programmable aberration correction, Journal of Biophotonics, Vol: 7, Pages: 29-36, ISSN: 1864-063X
We present a stimulated emission depletion (STED) microscope that provides 3‐D super resolution by simultaneous depletion using beams with both a helical phase profile for enhanced lateral resolution and an annular phase profile to enhance axial resolution. The 3‐D depletion point spread function is realised using a single spatial light modulator that can also be programmed to compensate for aberrations in the microscope and the sample. We apply it to demonstrate the first 3‐D super‐resolved imaging of an immunological synapse between a Natural Killer cell and its target cell.
Coda S, French PMW, Dunsby C, 2014, Oncology applications: Gastrointestinal cancer, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics, Pages: 379-386, ISBN: 9781439861677
Cancers of esophagus, stomach, and colon are among the most common cancers worldwide, accounting for a total of 2.2 million new cases each year (Boyle and Levin 2008). Prevention of these conditions is currently based on early detection of early-stage cancers or premalignant conditions during conventional white-light endoscopy (WLE). Today, there is a range of more sophisticated biophotonics techniques under development that aim to enhance the contrast of areas of concern beyond what is possible with conventional WLE. Commercially available techniques include high-definition endoscopy (HDE; Adler et al. 2009; Buchner 2010; Rex and Helbig 2007), narrow band imaging (NBI; Gono et al. 2004), magnifying chromoendoscopy (MCE; Kudo et al. 1996), autofluorescence (AF) imaging (AFI; Nakaniwa et al. 2005), and confocal laser endomicroscopy (CLE; Kiesslich et al. 2004; Wang et al. 2007).
Dunsby C, McGinty J, French P, 2014, Multidimensional fluorescence imaging of biological tissue, Biomedical Photonics Handbook, Second Edition: Fundamentals, Devices, and Techniques, Pages: 531-560, ISBN: 9781420085129
This chapter aims to review multidimensional fluorescence imaging (MDFI) technology and its application to biological tissue, with a particular emphasis on fluorescence lifetime imaging (FLIM) of biological tissue with examples from our work at Imperial College London. Fluorescence imaging is flourishing tremendously, partly driven by advances in laser and detector technology, partly by advances in labeling technologies such as genetically expressed fluorescent proteins, and partly by advances in computational analysis techniques. Increasingly, fluorescence instrumentation is developed to provide more information than just the localization or distribution of specific fluorescent molecules. Often, fluorescence signals are analyzed to provide information on the local fluorophore environment or to contrast different fluorophores in complex mixtures-as often occur in biological tissue. This trend to higher-content fluorescence imaging increasingly exploits MDFI and measurement capabilities with instrumentation that resolves fluorescence lifetime together with other spectroscopic parameters such as excitation and emission wavelength and polarization, providing image information in two or three spatial dimensions as well as with respect to elapsed time (Figure 18.1). However, caution should be exercised when acquiring such MDFI since photobleaching or experimental considerations usually impose a limited photon budget and/or a maximum image acquisition time and also present significant challenges with respect to data analysis and data management. These considerations are particularly important for real-time clinical diagnostic applications, for higher-throughput assays, and for the investigation of dynamic biological systems (Figure 18.1).
Marcu L, French PMW, Elson DS, 2014, Preface, ISBN: 9781439861677
Wide-field time-gated fluorescence lifetime imaging (FLIM) essentially entails illuminating a sample with an ultrashort pulse of excitation radiation and sampling the resulting time varying fluorescence “image” following excitation by acquiring a series of gated fluorescence intensity images recorded at different relative delays with respect to the excitation pulse. This is represented schematically in Figure 8.1. In the simplest case, a map of the mean fluorescence decay times across the field of view is obtained. If the sampling of the fluorescence decay profiles is appropriately detailed, then the entire fluorescence decay profile for each image pixel can be acquired, and the resulting data set can be fitted to complex temporal decay models. For example, a double exponential decay model is frequently used to analyze data from Förster resonant energy transfer (FRET) experiments. The acquisition of time-gated fluorescence intensity images requires a 2-D detector, normally a charge-coupled device (CCD) camera, and some kind of fast “shutter” able to sample fluorescence decay profiles on subnanosecond timescales. Such a “shutter” function cannot be provided by mechanical means or yet by electronic circuitry and is typically provided by optical image intensifiers whose gain can be modulated by varying the applied voltage.
Sonnefraud Y, Sivan Y, Sinclair HG, et al., 2014, Nanoparticle-assisted STED, theory, and experimental demonstration, Conference on Nanoimaging and Nanospectroscopy II, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
Nickdel MB, Lagarto JL, Kelly DJ, et al., 2014, Autofluorescence lifetime metrology for label-free detection of cartilage matrix degradation, Conference on Optical Biopsy XII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
Myatt SS, Kongsema M, Man CW-Y, et al., 2013, SUMOylation inhibits FOXM1 activity and delays mitotic transition, Oncogene, Vol: 33, Pages: 4316-4329, ISSN: 1476-5594
The forkhead box transcription factor FOXM1 is an essential effector of G2/M-phase transition, mitosis and the DNA damage response. As such, it is frequently deregulated during tumorigenesis. Here we report that FOXM1 is dynamically modified by SUMO1 but not by SUMO2/3 at multiple sites. We show that FOXM1 SUMOylation is enhanced in MCF-7 breast cancer cells in response to treatment with epirubicin and mitotic inhibitors. Mutation of five consensus conjugation motifs yielded a SUMOylation-deficient mutant FOXM1. Conversely, fusion of the E2 ligase Ubc9 to FOXM1 generated an auto-SUMOylating mutant (FOXM1-Ubc9). Analysis of wild-type FOXM1 and mutants revealed that SUMOylation inhibits FOXM1 activity, promotes translocation to the cytoplasm and enhances APC/Cdh1-mediated ubiquitination and degradation. Further, expression of the SUMOylation-deficient mutant enhanced cell proliferation compared with wild-type FOXM1, whereas the FOXM1-Ubc9 fusion protein resulted in persistent cyclin B1 expression and slowed the time from mitotic entry to exit. In summary, our findings suggest that SUMOylation attenuates FOXM1 activity and causes mitotic delay in cytotoxic drug response.
Roper JC, Yerolatsitis S, Birks TA, et al., 2013, Minimising group index variations in a multicore endoscope fibre
We describe a multicore endoscope fibre with minimised group index variation between cores that is obtained at a V parameter of 3. Tapering the fibre input enables us to achieve single-mode propagation. © OSA 2013.
Nickdel MB, Lagarto JL, Kelly DJ, et al., 2013, Detection of cartilage matrix degradation by autofluorescence lifetime, Spring Meeting of the British-Society-for-Matrix-Biology, Publisher: WILEY-BLACKWELL, Pages: A12-A13, ISSN: 0959-9673
Viessmann OM, Eckersley RJ, Christensen-Jeffries K, et al., 2013, Acoustic super-resolution with ultrasound and microbubbles, PHYSICS IN MEDICINE AND BIOLOGY, Vol: 58, Pages: 6447-6458, ISSN: 0031-9155
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- Citations: 188
Warren SC, Margineanu A, Alibhai D, et al., 2013, Rapid Global Fitting of Large Fluorescence Lifetime Imaging Microscopy Datasets, PLOS ONE, Vol: 8, ISSN: 1932-6203
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- Citations: 147
Seidenari S, Arginelli F, Dunsby C, et al., 2013, Multiphoton Laser Tomography and Fluorescence Lifetime Imaging of Melanoma: Morphologic Features and Quantitative Data for Sensitive and Specific Non-Invasive Diagnostics, PLOS ONE, Vol: 8, ISSN: 1932-6203
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- Citations: 59
Alibhai D, Kelly DJ, Warren S, et al., 2013, Automated fluorescence lifetime imaging plate reader and its application to Forster resonant energy transfer readout of Gag protein aggregation, Journal of Biophotonics, Vol: 6, Pages: 398-408, ISSN: 1864-0648
Fluorescence lifetime measurements can provide quantitativereadouts of local fluorophore environment andcan be applied to biomolecular interactions via Fo¨ rsterresonant energy transfer (FRET). Fluorescence lifetimeimaging (FLIM) can therefore provide a high contentanalysis (HCA) modality to map protein-protein interactions(PPIs) with applications in drug discovery, systemsbiology and basic research. We present here an automatedmultiwell plate reader able to perform rapid unsupervisedoptically sectioned FLIM of fixed and livebiological samples and illustrate its potential to assayPPIs through application to Gag protein aggregationduring the HIV life cycle. We demonstrate both heteroFRETand homo-FRET readouts of protein aggregationand report the first quantitative evaluation of a FLIMHCA assay by generating dose response curves throughaddition of an inhibitor of Gag myristoylation. Z0 factorsexceeding 0.6 are realised for this FLIM FRET assay.Fluorescence lifetime plate map with representativeimages of high and low FRET cells and correspondingdose response plot.
Arginelli F, Manfredini M, Bassoli S, et al., 2013, High resolution diagnosis of common nevi by multiphoton laser tomography and fluorescence lifetime imaging, SKIN RESEARCH AND TECHNOLOGY, Vol: 19, Pages: 194-204, ISSN: 0909-752X
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- Citations: 9
Manfredini M, Arginelli F, Dunsby C, et al., 2013, High-resolution imaging of basal cell carcinoma: a comparison between multiphoton microscopy with fluorescence lifetime imaging and reflectance confocal microscopy, SKIN RESEARCH AND TECHNOLOGY, Vol: 19, Pages: E433-E443, ISSN: 0909-752X
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- Citations: 21
Coda S, Kelly DJ, Lagarto JL, et al., 2013, Autofluorescence lifetime imaging and metrology for medical research and clinical diagnosis
We report the development of instrumentation to utilise autofluorescence lifetime for the study and diagnosis of disease including cancer and osteoarthritis. ©2013 The Optical Society (OSA).
Warren S, Kimberley C, Margineanu A, et al., 2013, Flim-fret of cell signalling in chemotaxis
We demonstrate the application of Fluorescence Lifetime Imaging (FLIM) to read out Förster resonant energy transfer (FRET) based biosensors for studying the spatio-temporal dynamics of signalling pathways in cells undergoing chemotaxis. ©2013 The Optical Society (OSA).
Kelly DJ, Alibhai D, Warren S, et al., 2013, An automated flim multiwell plate reader for high content analysis
We report an automated fluorescence lifetime imaging multiwell plate reader for high content analysis, capable of subcellular mapping of protein interactions. This instrument can acquire FLIM data from 96 wells in less than 15 minutes.©2013 The Optical Society (OSA).
Manning HB, Nickdel MB, Yamamoto K, et al., 2013, Detection of cartilage matrix degradation by autofluorescence lifetime, MATRIX BIOLOGY, Vol: 32, Pages: 32-38, ISSN: 0945-053X
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- Citations: 31
Roper JC, Yerolatsitis S, Birks TA, et al., 2013, Minimising group index variations in a multicore endoscope fibre, Conference on Lasers and Electro-Optics (CLEO), Publisher: IEEE, ISSN: 2160-9020
Martins M, Warren S, Kimberley C, et al., 2012, Activity of PLCε contributes to chemotaxis of fibroblasts towards PDGF, JOURNAL OF CELL SCIENCE, Vol: 125, Pages: 5758-5769, ISSN: 0021-9533
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- Citations: 15
Laine R, Stuckey DW, Manning H, et al., 2012, Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores, PLOS ONE, Vol: 7, ISSN: 1932-6203
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- Citations: 20
Seidenari S, Arginelli F, Dunsby C, et al., 2012, Multiphoton laser tomography and fluorescence lifetime imaging of basal cell carcinoma: morphologic features for non-invasive diagnostics, EXPERIMENTAL DERMATOLOGY, Vol: 21, Pages: 831-836, ISSN: 0906-6705
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- Citations: 32
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