Publications
272 results found
Talbot CB, Patalay R, Munro I, et al., 2011, A multispectral FLIM tomograph for <i>in vivo</i> imaging of skin cancer, Conference on Multiphoton Microscopy in the Biomedical Sciences XI, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 3
McGinty J, Talbot C, Owen D, et al., 2011, Fluorescence Lifetime Imaging Microscopy, Endoscopy and Tomography, Editors: Boas, Pitris, Ramanujam, ISBN: 1420090364
Patalay R, Talbot C, Alexandrov Y, et al., 2011, Non-invasive imaging of skin cancer with fluorescence lifetime imaging using two photon tomography, Conference on Clinical and Biomedical Spectroscopy and Imaging II, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 3
McGinty J, Galletly NP, Dunsby C, et al., 2010, Wide-field fluorescence lifetime imaging of cancer, BIOMEDICAL OPTICS EXPRESS, Vol: 1, Pages: 627-640, ISSN: 2156-7085
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- Citations: 77
Talbot C, McGinty J, McGhee E, et al., 2010, Fluorescence Lifetime Imaging and Metrology for Biomedicine, Handbook of Photonics for Biomedical Science, ISBN: 978-1439806289
Dunsby C, French PMW, 2010, Biophotonics applications of supercontinuum generation, Supercontinuum Generation in Optical Fibers, Pages: 349-373, ISBN: 9780521514804
Introduction Continua generated using high pulse energy laser systems to create broad spectra (Alfano and Shapiro, 1970) have been used for spectroscopy for many years (e.g. Busch et al., 1973). The application of fibre-generated continua to spectroscopy was suggested as early as 1976 in work by Lin and Stolen (1976) where a continuum spanning ∼450-600 nm was generated in a step-index fibre pumped by a nitrogen pumped dye laser. Since the demonstration of supercontinuum generation in microstructured optical fibres (MOF), however, the range of spectroscopic and imaging applications has increased enormously, owing to the high average powers, unprecedented spectral width and relatively low cost and low complexity of such sources. This chapter specifically focuses on the applications of supercontinua generated in MOFs and, in particular, on applications in biophotonics. MOF supercontinuum sources can be broadly grouped into three categories according to whether the laser pump source emits femtosecond pulses, picosecond-nanosecond pulses or cw radiation. In general terms, sub-ps pulses can produce broad supercontinua spanning from the UV to the NIR but the peak intensity damage threshold at the input end of the microstructured optical fibre limits the maximum average power that can be obtained in the supercontinuum to ∼<0.5 W with typically ∼<0.5 mW/nm available in the visible spectrum. The use of pump lasers with longer ps-ns pulses can significantly increase the maximum available average power before the onset of damage in the MOF such that high power supercontinua with several mW/nm in the visible can be achieved (e.g. Rulkov et al., 2005).
Kennedy GT, Manning HB, Elson DS, et al., 2010, A fluorescence lifetime imaging scanning confocal endomicroscope, JOURNAL OF BIOPHOTONICS, Vol: 3, Pages: 103-107, ISSN: 1864-063X
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- Citations: 35
Tang M-X, Elson DS, Li R, et al., 2010, Photoacoustics, thermoacoustics, and acousto-optics for biomedical imaging, PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART H-JOURNAL OF ENGINEERING IN MEDICINE, Vol: 224, Pages: 291-306, ISSN: 0954-4119
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- Citations: 13
Robinson T, Manning HB, Dunsby C, et al., 2010, Investigating fast enzyme-DNA kinetics using multidimensional fluorescence imaging and microfluidics, Conference on Microfluidics, BioMEMS, and Medical Microsystems VIII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 1
McGinty J, Requejo-Isidro J, Munro I, et al., 2009, Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 42, ISSN: 0022-3727
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- Citations: 19
McGinty J, Dunsby C, Auksorius E, et al., 2009, Chapter 4 Multidimensional fluorescence imaging
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- Citations: 3
Schaerli Y, Wootton RC, Robinson T, et al., 2009, Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets, ANALYTICAL CHEMISTRY, Vol: 81, Pages: 302-306, ISSN: 0003-2700
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- Citations: 221
McGinty J, Dunsby C, Auksorius E, et al., 2009, Multidimensional fluorescence imaging, Laboratory Techniques in Biochemistry and Molecular Biology (FLIM and FRET techniques), Editors: Gadella, Publisher: Elsevier, ISBN: 9780080915128
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips.
McGinty J, Dunsby C, Auksorius E, et al., 2009, Multidimensional fluorescence imaging, FRET AND FLIM TECHNIQUES, Editors: Gadella, Publisher: ELSEVIER SCIENCE BV, Pages: 133-169, ISBN: 978-0-08-054958-3
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- Citations: 2
Robinson T, Schaerli Y, Wootton R, et al., 2009, Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging, LAB ON A CHIP, Vol: 9, Pages: 3437-3441, ISSN: 1473-0197
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- Citations: 29
Li R, Elson DS, Dunsby C, et al., 2009, A study on optical modulation signal and tissue displacement in Ultrasound Modulated Optical Tomography, Conference on Photons Plus Ultrasound - Imaging and Sensing 2009, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 1
Dunsby C, 2008, Optically sectioned imaging by oblique plane microscopy, OPTICS EXPRESS, Vol: 16, Pages: 20306-20316, ISSN: 1094-4087
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- Citations: 176
Talbot C, McGinty J, McGhee E, et al., 2008, High speed, optically sectioned fluorescence lifetime imaging utilizing time-gated nipkow disk or multifocal multiphoton time correlated single photon counting microscopy
We report two optically sectioned fluorescence lifetime systems that exhibit better signal to noise per unit time than conventional time correlated single photon counting systems. Both systems are applied to biologically relevant samples. ©2007 Optical Society of America.
Manning HB, Kennedy GT, Owen DM, et al., 2008, A compact, multidimensional spectrofluorometer exploiting supercontinuum generation, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 494-505, ISSN: 1864-063X
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- Citations: 35
Talbot CB, McGinty J, Grant DM, et al., 2008, High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 514-521, ISSN: 1864-063X
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- Citations: 40
Grant DM, Zhang W, McGhee EJ, et al., 2008, Multiplexed FRET to Image Multiple Signaling Events in Live Cells, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L69-L71, ISSN: 0006-3495
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- Citations: 79
Oddos S, Dunsby C, Purbhoo MA, et al., 2008, High-Speed High-Resolution Imaging of Intercellular Immune Synapses Using Optical Tweezers, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L66-L68, ISSN: 0006-3495
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- Citations: 57
McGinty J, Tahir KR, Laine R, et al., 2008, Fluorescence lifetime optical projection tomography, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 390-394, ISSN: 1864-063X
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- Citations: 39
Robinson T, Valluri P, Manning HB, et al., 2008, Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 1887-1889, ISSN: 0146-9592
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- Citations: 19
Galletly NP, McGinty J, Dunsby C, et al., 2008, Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin, BRITISH JOURNAL OF DERMATOLOGY, Vol: 159, Pages: 152-161, ISSN: 0007-0963
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- Citations: 104
Auksorius E, Boruah BR, Dunsby C, et al., 2008, Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 113-115, ISSN: 0146-9592
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- Citations: 143
McGinty J, Tahir KB, Soloviev VY, et al., 2008, Time-domain fluorescence lifetime tomography
We present a platform for fluorescence lifetime tomography utilising tuneable supercontinuum excitation and wide-field time-gated technology. Applied to optical projection and diffuse fluorescence tomography, we demonstrate 3D time-resolved fluorescence reconstruction in transparent and scattering phantoms.
Owen DM, Auksorius E, Manning HB, et al., 2007, Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source, OPTICS LETTERS, Vol: 32, Pages: 3408-3410, ISSN: 0146-9592
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- Citations: 54
De Beule PAA, Dunsby C, Galletly NP, et al., 2007, A hyperspectral fluorescence lifetime probe for skin cancer diagnosis, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 78, ISSN: 0034-6748
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- Citations: 70
Grant DM, McGinty J, McGhee EJ, et al., 2007, High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events, Optics Express, Vol: 15, Pages: 16656-16673
We present a time domain optically sectioned fluorescence lifetime imaging (FLIM) microscope developed for high-speed live cell imaging. This single photon excited system combines wide field parallel pixel detection with confocal sectioning utilizing spinning Nipkow disc microscopy. It can acquire fluorescence lifetime images of live cells at up to 10 frames per second (fps), permitting high-speed FLIM of cell dynamics and protein interactions with potential for high throughput cell imaging and screening applications. We demonstrate the application of this FLIM microscope to real-time monitoring of changes in lipid order in cell membranes following cholesterol depletion using cyclodextrin and to the activation of the small GTP-ase Ras in live cells using FRET. © 2007 Optical Society of America.
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