Imperial College London
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Professor Chris Dunsby

Faculty of Natural Sciences, Department of Physics

Professor of Biomedical Optics
 
 
 
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Contact

 

+44 (0)20 7594 7755christopher.dunsby Website

 
 
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Location

 

622Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lagarto:2018:10.1364/BOE.9.004961,
author = {Lagarto, J and Dyer, B and Talbot, C and Peters, N and French, P and Lyon, A and Dunsby, C},
doi = {10.1364/BOE.9.004961},
journal = {Biomedical Optics Express},
pages = {4978--4978},
title = {Characterization of NAD(P)H and FAD autofluorescence signatures in a Langendorff isolated-perfused rat heart model},
url = {http://dx.doi.org/10.1364/BOE.9.004961},
volume = {9},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Autofluorescence spectroscopy is a promising label-free approach to characterize biological samples with demonstrated potential to report structural and biochemical alterations in tissues in a number of clinical applications. We report a characterization of the ex vivo autofluorescence fingerprint of cardiac tissue, exploiting a Langendorff-perfused isolated rat heart model to induce physiological insults to the heart, with a view to understanding how metabolic alterations affect the autofluorescence signals. Changes in the autofluorescence intensity and lifetime signatures associated with reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) were characterized during oxygen- or glucose-depletion protocols. Results suggest that both NAD(P)H and FAD autofluorescence intensity and lifetime parameters are sensitive to changes in the metabolic state of the heart owing to oxygen deprivation. We also observed changes in NAD(P)H fluorescence intensity and FAD lifetime parameter on reperfusion of oxygen, which might provide information on reperfusion injury, and permanent tissue damage or changes to the tissue during recovery from oxygen deprivation. We found that changes in the autofluorescence signature following glucose-depletion are, in general, less pronounced, and most clearly visible in NAD(P)H related parameters. Overall, the results reported in this investigation can serve as baseline for future investigations of cardiac tissue involving autofluorescence measurements.
AU  - Lagarto,J
AU  - Dyer,B
AU  - Talbot,C
AU  - Peters,N
AU  - French,P
AU  - Lyon,A
AU  - Dunsby,C
DO  - 10.1364/BOE.9.004961
EP  - 4978
PY  - 2018///
SN  - 2156-7085
SP  - 4978
TI  - Characterization of NAD(P)H and FAD autofluorescence signatures in a Langendorff isolated-perfused rat heart model
T2  - Biomedical Optics Express
UR  - http://dx.doi.org/10.1364/BOE.9.004961
UR  - http://hdl.handle.net/10044/1/63497
VL  - 9
ER  -
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