Imperial College London
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Professor Chris Dunsby

Faculty of Natural Sciences, Department of Physics

Professor of Biomedical Optics
 
 
 
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Contact

 

+44 (0)20 7594 7755christopher.dunsby Website

 
 
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Location

 

622Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lagarto:2019:10.1364/BOE.10.003506,
author = {Lagarto, J and Dyer, B and Dunsby, C and Peters, N and French, P and Dunsby, C and Lyon, A},
doi = {10.1364/BOE.10.003506},
journal = {Biomedical Optics Express},
pages = {3506--3521},
title = {In vivo label-free optical monitoring of structural and metabolic remodeling of myocardium following infarction},
url = {http://dx.doi.org/10.1364/BOE.10.003506},
volume = {10},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cardiac  remodeling  following  myocardial  infarction  (MI)  involves  structural  and  functional alterations in the infarcted and remote viable myocardium that can ultimately lead to  heart  failure.  The  underlying  mechanisms  are  not  fully  understood  and,  following  our  previous  study  of  the  autofluorescence  lifetime  and  diffuse  reflectance  signatures  of  the  myocardium in vivo at 16 weeks post MI in rats [Biomed. Opt. Express 6(2), 324 (2015)], we here present data obtained at 1, 2 and 4 weeks post myocardial infarction that help follow the temporal  progression  of  these  changes.  Our  results  demonstrate  that  both  structural  and  metabolic  changes  in  the  heart  can  be  monitored  from  the  earliest  time  points  following  MI  using  label-free  optical  readouts,  not  only  in  the  region  of  infarction  but  also  in  the  remote  non-infarcted myocardium. Changes in the autofluorescence intensity and lifetime parameters associated  with  collagen  type  I  autofluorescence  were  indicative  of  progressive  collagen  deposition  in  tissue  that  was  most  pronounced  at  earlier  time  points  and  in  the  region  of  infarction.  In  addition  to  significant  collagen  deposition  in  infarcted  and  non-infarcted  myocardium,  we  also  report  changes  in  the  autofluorescence  parameters  associated  with  reduced  nicotinamide  adenine  (phosphate)  dinucleotide  (NAD(P)H)  and  flavin  adenine  dinucleotide  (FAD),  which  we  associate  with  metabolic  alterations  throughout  the  heart.  Parallel measurements of the diffuse reflectance spectra indicated an increased contribution of reduced cytochrome c. Our findings suggest that combining time-resolved spectrofluorometry and diffuse reflectance spectroscopy could provide a useful means to monitor cardiac function in vivo at the time of surgery.
AU  - Lagarto,J
AU  - Dyer,B
AU  - Dunsby,C
AU  - Peters,N
AU  - French,P
AU  - Dunsby,C
AU  - Lyon,A
DO  - 10.1364/BOE.10.003506
EP  - 3521
PY  - 2019///
SN  - 2156-7085
SP  - 3506
TI  - In vivo label-free optical monitoring of structural and metabolic remodeling of myocardium following infarction
T2  - Biomedical Optics Express
UR  - http://dx.doi.org/10.1364/BOE.10.003506
UR  - https://www.osapublishing.org/boe/abstract.cfm?uri=boe-10-7-3506
UR  - http://hdl.handle.net/10044/1/70738
VL  - 10
ER  -
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