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@article{Sparks:2020:10.1364/boe.409781,
author = {Sparks, H and Dent, L and Bakal, C and Behrens, A and Salbreux, G and Dunsby, C},
doi = {10.1364/boe.409781},
journal = {Biomedical Optics Express},
pages = {7204--7220},
title = {Dual-view oblique plane microscopy (dOPM)},
url = {http://dx.doi.org/10.1364/boe.409781},
volume = {11},
year = {2020}
}

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TY  - JOUR
AB  - We present a new folded dual-view oblique plane microscopy (OPM) techniquetermed dOPM that enables two orthogonal views of the sample to be obtained by translating apair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primaryobjective, deconvolved image volumes of 200 nm beads were measured to have full width athalf maxima (FWHM) of 0.35±0.04 μm and 0.39±0.02 μm laterally and 0.81±0.07 μm axially.The measured z-sectioning value was 1.33±0.45 μm using light-sheet FWHM in the frames ofthe two views of 4.99±0.58 μm and 4.89±0.63 μm. To qualitatively demonstrate that the systemcan reduce shadow artefacts while providing a more isotropic resolution, a multi-cellularspheroid approximately 100 μm in diameter was imaged.
AU  - Sparks,H
AU  - Dent,L
AU  - Bakal,C
AU  - Behrens,A
AU  - Salbreux,G
AU  - Dunsby,C
DO  - 10.1364/boe.409781
EP  - 7220
PY  - 2020///
SN  - 2156-7085
SP  - 7204
TI  - Dual-view oblique plane microscopy (dOPM)
T2  - Biomedical Optics Express
UR  - http://dx.doi.org/10.1364/boe.409781
UR  - https://www.osapublishing.org/boe/fulltext.cfm?uri=boe-11-12-7204&id=442746
UR  - http://hdl.handle.net/10044/1/83985
VL  - 11
ER  -

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