Imperial College London
Portrait Picture

Professor Chris Dunsby

Faculty of Natural Sciences, Department of Physics

Professor of Biomedical Optics
 
 
 
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Contact

 

+44 (0)20 7594 7755christopher.dunsby Website

 
 
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Location

 

622Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cannon:2021:10.1364/OSAC.423082,
author = {Cannon, T and Lagarto, J and Dyer, B and Garcia, Castano E and Kelly, D and Peters, N and Lyon, A and French, P and Dunsby, C},
doi = {10.1364/OSAC.423082},
journal = {Optical Society of America Continuum},
pages = {1610--1625},
title = {Characterisation of NADH fluorescence properties under one-photon excitation with respect to temperature, pH and binding to lactate dehydrogenase},
url = {http://dx.doi.org/10.1364/OSAC.423082},
volume = {4},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Reduced nicotinamide adenine dinucleotide (NADH) is the principal electron donor in glycolysis and oxidative metabolism and is thus recognized as a key biomarker for probing metabolic state. While the fluorescence characteristics of NADH have been investigated extensively, there are discrepancies in the published data due to diverse experimental conditions, instrumentation and microenvironmental parameters that can affect NADH fluorescence. Using a cuvette-based time-resolved spectrofluorimeter employing one-photon excitation at 375 nm, we characterized the fluorescence intensity, lifetime, spectral response, anisotropy and time-resolved anisotropy of NADH in aqueous solution under varying microenvironmental conditions, namely temperature, pH, and binding to lactate dehydrogenase (LDH). Our results demonstrate how temperature, pH, and binding partners each impact the fluorescence signature of NADH and highlight the complexity of the fluorescence data when different parameters produce competing effects. We hope that the data presented in this study will provide a reference for potential sources of variation in experiments measuring NADH fluorescence.
AU  - Cannon,T
AU  - Lagarto,J
AU  - Dyer,B
AU  - Garcia,Castano E
AU  - Kelly,D
AU  - Peters,N
AU  - Lyon,A
AU  - French,P
AU  - Dunsby,C
DO  - 10.1364/OSAC.423082
EP  - 1625
PY  - 2021///
SP  - 1610
TI  - Characterisation of NADH fluorescence properties under one-photon excitation with respect to temperature, pH and binding to lactate dehydrogenase
T2  - Optical Society of America Continuum
UR  - http://dx.doi.org/10.1364/OSAC.423082
UR  - https://www.osapublishing.org/osac/fulltext.cfm?uri=osac-4-5-1610&id=450919
UR  - http://hdl.handle.net/10044/1/89545
VL  - 4
ER  -
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