Imperial College London
Portrait Picture

Professor Cleo Kontoravdi

Faculty of Engineering, Department of Chemical Engineering

Professor of Biological Systems Engineering
 
 
 
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Contact

 

+44 (0)20 7594 6655cleo.kontoravdi98 Website

 
 
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Location

 

310ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Alhuthali:2021:10.3390/ijms22073290,
author = {Alhuthali, S and Kotidis, P and Kontoravdi, K},
doi = {10.3390/ijms22073290},
journal = {International Journal of Molecular Sciences},
title = {Osmolality effects on CHO cell growth, cell volume and antibody productivity and glycosylation},
url = {http://dx.doi.org/10.3390/ijms22073290},
volume = {22},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The addition of nutrients and accumulation of metabolites in a fed-batch culture of Chinese hamster ovary (CHO) cells leads to an increase in extracellular osmolality in late stage culture. Herein, we explore the effect of osmolality on CHO cell growth, specific monoclonal antibody (mAb) productivity and glycosylation achieved with the addition of NaCl or the supplementation of a commercial feed. Although both methods lead to an increase in specific antibody productivity, they have different effects on cell growth and antibody production. Osmolality modulation using NaCl up to 470 mOsm kg−1 had a consistently positive effect on specific antibody productivity and titre. The addition of the commercial feed achieved variable results: specific mAb productivity was increased, yet cell growth rate was significantly compromised at high osmolality values. As a result, Feed C addition to 410 mOsm kg−1 was the only condition that achieved a significantly higher mAb titre compared to the control. Additionally, Feed C supplementation resulted in a significant reduction in galactosylated antibody structures. Cell volume was found to be positively correlated to osmolality; however, osmolality alone could not account for observed changes in average cell diameter without considering cell cycle variations. These results help delineate the overall effect of osmolality on titre and highlight the potentially negative effect of overfeeding on cell growth.
AU  - Alhuthali,S
AU  - Kotidis,P
AU  - Kontoravdi,K
DO  - 10.3390/ijms22073290
PY  - 2021///
SN  - 1422-0067
TI  - Osmolality effects on CHO cell growth, cell volume and antibody productivity and glycosylation
T2  - International Journal of Molecular Sciences
UR  - http://dx.doi.org/10.3390/ijms22073290
UR  - http://hdl.handle.net/10044/1/88797
VL  - 22
ER  -
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