Publications

BibTex format

@article{Holden:2013:10.1101/gr.147710.112,
author = {Holden, MTG and Hsu, L-Y and Kurt, K and Weinert, LA and Mather, AE and Harris, SR and Strommenger, B and Layer, F and Witte, W and de, Lencastre H and Skov, R and Westh, H and Zemlickova, H and Coombs, G and Kearns, AM and Hill, RLR and Edgeworth, J and Gould, I and Gant, V and Cooke, J and Edwards, GF and McAdam, PR and Templeton, KE and McCann, A and Zhou, Z and Castillo-Ramirez, S and Feil, EJ and Hudson, LO and Enright, MC and Balloux, F and Aanensen, DM and Spratt, BG and Fitzgerald, JR and Parkhill, J and Achtman, M and Bentley, SD and Nuebel, U},
doi = {10.1101/gr.147710.112},
journal = {Genome Research},
pages = {653--664},
title = {A genomic portrait of the emergence, evolution, and global spread of a methicillin-resistant Staphylococcus aureus pandemic},
url = {http://dx.doi.org/10.1101/gr.147710.112},
volume = {23},
year = {2013}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The widespread use of antibiotics in association with high-density clinical care has driven the emergence of drug-resistant bacteria that are adapted to thrive in hospitalized patients. Of particular concern are globally disseminated methicillin-resistant Staphylococcus aureus (MRSA) clones that cause outbreaks and epidemics associated with health care. The most rapidly spreading and tenacious health-care-associated clone in Europe currently is EMRSA-15, which was first detected in the UK in the early 1990s and subsequently spread throughout Europe and beyond. Using phylogenomic methods to analyze the genome sequences for 193 S. aureus isolates, we were able to show that the current pandemic population of EMRSA-15 descends from a health-care-associated MRSA epidemic that spread throughout England in the 1980s, which had itself previously emerged from a primarily community-associated methicillin-sensitive population. The emergence of fluoroquinolone resistance in this EMRSA-15 subclone in the English Midlands during the mid-1980s appears to have played a key role in triggering pandemic spread, and occurred shortly after the first clinical trials of this drug. Genome-based coalescence analysis estimated that the population of this subclone over the last 20 yr has grown four times faster than its progenitor. Using comparative genomic analysis we identified the molecular genetic basis of 99.8% of the antimicrobial resistance phenotypes of the isolates, highlighting the potential of pathogen genome sequencing as a diagnostic tool. We document the genetic changes associated with adaptation to the hospital environment and with increasing drug resistance over time, and how MRSA evolution likely has been influenced by country-specific drug use regimens.
AU  - Holden,MTG
AU  - Hsu,L-Y
AU  - Kurt,K
AU  - Weinert,LA
AU  - Mather,AE
AU  - Harris,SR
AU  - Strommenger,B
AU  - Layer,F
AU  - Witte,W
AU  - de,Lencastre H
AU  - Skov,R
AU  - Westh,H
AU  - Zemlickova,H
AU  - Coombs,G
AU  - Kearns,AM
AU  - Hill,RLR
AU  - Edgeworth,J
AU  - Gould,I
AU  - Gant,V
AU  - Cooke,J
AU  - Edwards,GF
AU  - McAdam,PR
AU  - Templeton,KE
AU  - McCann,A
AU  - Zhou,Z
AU  - Castillo-Ramirez,S
AU  - Feil,EJ
AU  - Hudson,LO
AU  - Enright,MC
AU  - Balloux,F
AU  - Aanensen,DM
AU  - Spratt,BG
AU  - Fitzgerald,JR
AU  - Parkhill,J
AU  - Achtman,M
AU  - Bentley,SD
AU  - Nuebel,U
DO  - 10.1101/gr.147710.112
EP  - 664
PY  - 2013///
SN  - 1549-5469
SP  - 653
TI  - A genomic portrait of the emergence, evolution, and global spread of a methicillin-resistant Staphylococcus aureus pandemic
T2  - Genome Research
UR  - http://dx.doi.org/10.1101/gr.147710.112
UR  - http://hdl.handle.net/10044/1/30649
VL  - 23
ER  -

Download

DrDavidAanensen

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)