Publications
191 results found
Lenz MO, Brown ACN, Auksorius E, et al., 2011, A STED-FLIM microscope applied to imaging the Natural Killer cell immune synapse, Conference on Multiphoton Microscopy in the Biomedical Sciences XI, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 5
Borhis G, Purbhoo M, Ahmed PS, et al., 2010, Effects of MHC class I peptides on KIR2DL3 clustering and signalling, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL PUBLISHING, INC, Pages: 134-134, ISSN: 0019-2805
Koehler K, Xiong S, Brzostek J, et al., 2010, Matched sizes of activating and inhibitory receptor/ligand pairs are required for optimal signal integration by human natural killer cells, PLoS ONE, Vol: 5, ISSN: 1932-6203
It has been suggested that receptor-ligand complexes segregate or co-localise within immune synapses according to their size, and this is important for receptor signaling. Here, we set out to test the importance of receptor-ligand complex dimensions for immune surveillance of target cells by human Natural Killer (NK) cells. NK cell activation is regulated by integrating signals from activating receptors, such as NKG2D, and inhibitory receptors, such as KIR2DL1. Elongating the NKG2D ligand MICA reduced its ability to trigger NK cell activation. Conversely, elongation of KIR2DL1 ligand HLA-C reduced its ability to inhibit NK cells. Whereas normal-sized HLA-C was most effective at inhibiting activation by normal-length MICA, only elongated HLA-C could inhibit activation by elongated MICA. Moreover, HLA-C and MICA that were matched in size co-localised, whereas HLA-C and MICA that were different in size were segregated. These results demonstrate that receptor-ligand dimensions are important in NK cell recognition, and suggest that optimal integration of activating and inhibitory receptor signals requires the receptor-ligand complexes to have similar dimensions.
Owen DM, Oddos S, Kumar S, et al., 2010, High plasma membrane lipid order imaged at the immunological synapse periphery in live T cells, MOLECULAR MEMBRANE BIOLOGY, Vol: 27, Pages: 178-189, ISSN: 0968-7688
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- Citations: 64
Eissmann P, Evans JH, Mehrabi M, et al., 2010, Multiple Mechanisms Downstream of TLR-4 Stimulation Allow Expression of NKG2D Ligands To Facilitate Macrophage/NK Cell Crosstalk, JOURNAL OF IMMUNOLOGY, Vol: 184, Pages: 6901-6909, ISSN: 0022-1767
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- Citations: 63
Davis DM, 2010, Budget cuts: funding needed for startling new discoveries too, NATURE, Vol: 465, Pages: 547-547, ISSN: 0028-0836
Horowitz A, Newman KC, Evans JH, et al., 2010, Cross-Talk between T Cells and NK Cells Generates Rapid Effector Responses to <i>Plasmodium falciparum</i>-Infected Erythrocytes, JOURNAL OF IMMUNOLOGY, Vol: 184, Pages: 6043-6052, ISSN: 0022-1767
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- Citations: 102
Liu H, Purbhoo MA, Davis DM, et al., 2010, SH2 domain containing leukocyte phosphoprotein of 76-kDa (SLP-76) feedback regulation of ZAP-70 microclustering, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 10166-10171, ISSN: 0027-8424
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- Citations: 16
Fadda L, Borhis G, Ahmed P, et al., 2010, Peptide antagonism as a mechanism for NK cell activation, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 10160-10165, ISSN: 0027-8424
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- Citations: 124
Yang J, Koehler K, Davis DM, et al., 2010, An improved strip FRAP method for estimating diffusion coefficients: correcting for the degree of photobleaching, JOURNAL OF MICROSCOPY, Vol: 238, Pages: 240-253, ISSN: 0022-2720
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- Citations: 14
Purbhoo MA, Liu H, Oddos S, et al., 2010, Dynamics of Subsynaptic Vesicles and Surface Microclusters at the Immunological Synapse, SCIENCE SIGNALING, Vol: 3, ISSN: 1945-0877
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- Citations: 101
Chauveau A, Aucher A, Eissmann P, et al., 2010, Membrane nanotubes facilitate long-distance interactions between natural killer cells and target cells, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 5545-5550, ISSN: 0027-8424
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- Citations: 152
Eissmann P, Davis DM, 2010, Inhibitory and regulatory immune synapses, ISBN: 9783642038570
Cell contact-dependent inhibition and regulation of immune responses play an essential role in balancing the need for rapid and efficient responses to a wide variety of pathological challenges, while at the same time maintaining self-tolerance. Much attention has been given to immune synapses that lead to the activation of, for example, cell-mediated cytotoxicity, and here we compare the supramolecular dynamics of synapses that lead to inhibition or regulatory functions. We focus on natural killer cells where such different synapses have been best studied. An emergent principle is that inhibition or regulatory responses are commonly achieved by selective recruitment of signalling proteins to the synapse and exclusion of membrane-proximal intracellular proteins needed for activation. We also discuss evidence that an inhibitory synapse triggers or maintains effector cells in a migratory configuration, which serves to break the synapse before the steps needed for effector cell activation can be completed. This model implies that the concept of kinetic-proofreading, previously used to describe activation of individual T-cell receptors, can also apply in determining the outcome of intercellular conjugation. © 2010 Springer-Verlag Berlin Heidelberg.
Eissmann P, Davis DM, 2010, Inhibitory and Regulatory Immune Synapses, IMMUNOLOGICAL SYNAPSE, Vol: 340, Pages: 63-79, ISSN: 0070-217X
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- Citations: 20
Davis DM, 2009, Mechanisms and functions for the duration of intercellular contacts made by lymphocytes, NATURE REVIEWS IMMUNOLOGY, Vol: 9, Pages: 543-555, ISSN: 1474-1733
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- Citations: 69
Culley FJ, Johnson M, Evans JH, et al., 2009, Natural Killer Cell Signal Integration Balances Synapse Symmetry and Migration, PLOS BIOLOGY, Vol: 7, ISSN: 1544-9173
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- Citations: 72
Benninger RKP, Vanherberghen B, Young S, et al., 2009, Live Cell Linear Dichroism Imaging Reveals Extensive Membrane Ruffling within the Docking Structure of Natural Killer Cell Immune Synapses, BIOPHYSICAL JOURNAL, Vol: 96, Pages: L13-L15, ISSN: 0006-3495
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- Citations: 25
Talbot C, McGinty J, McGhee E, et al., 2008, High speed, optically sectioned fluorescence lifetime imaging utilizing time-gated nipkow disk or multifocal multiphoton time correlated single photon counting microscopy
We report two optically sectioned fluorescence lifetime systems that exhibit better signal to noise per unit time than conventional time correlated single photon counting systems. Both systems are applied to biologically relevant samples. ©2007 Optical Society of America.
Oddos S, Dunsby C, Purbhoo MA, et al., 2008, High-Speed High-Resolution Imaging of Intercellular Immune Synapses Using Optical Tweezers, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L66-L68, ISSN: 0006-3495
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- Citations: 57
Davis DM, Sowinski S, 2008, Membrane nanotubes: dynamic long-distance connections between animal cells, NATURE REVIEWS MOLECULAR CELL BIOLOGY, Vol: 9, Pages: 431-436, ISSN: 1471-0072
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- Citations: 290
Sowinski S, Jolly C, Berninghausen O, et al., 2008, Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission, NATURE CELL BIOLOGY, Vol: 10, Pages: 211-219, ISSN: 1465-7392
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- Citations: 568
Newman KC, Korbell DS, Horowitz A, et al., 2008, Innate immunity to malaria., 3rd Molecular Approaches to Malaria Meeting (MAM 2008), Publisher: PERGAMON-ELSEVIER SCIENCE LTD, Pages: S32-S33, ISSN: 0020-7519
Stern-Ginossar N, Nedvetzki S, Markel G, et al., 2007, Intercellular transfer of carcinoembryonic antigen from tumor cells to NK cells, JOURNAL OF IMMUNOLOGY, Vol: 179, Pages: 4424-4434, ISSN: 0022-1767
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- Citations: 12
Kumar S, Dunsby C, De Beule PAA, et al., 2007, Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging, Optics Express, Vol: 15, Pages: 12548-12561
We report a multifocal multiphoton time-correlated single photon counting (TCSPC) fluorescence lifetime imaging (FLIM) microscope system that uses a 16 channel multi-anode PMT detector. Multiphoton excitation minimizes out-of-focus photobleaching, multifocal excitation reduces non-linear in-plane photobleaching effects and TCSPC electronics provide photon-efficient detection of the fluorescence decay profile. TCSPC detection is less prone to bleaching- and movementinduced artefacts compared to wide-field time-gated or frequency-domain FLIM. This microscope is therefore capable of acquiring 3-D FLIM images at significantly increased speeds compared to single beam multiphoton microscopy and we demonstrate this with live cells expressing a GFP tagged protein. We also apply this system to time-lapse FLIM of NAD(P)H autofluorescence in single live cells and report measurements on the change in the fluorescence decay profile following the application of a known metabolic inhibitor.
Williams GS, Collinson LM, Brzostek J, et al., 2007, Membranous structures transfer cell surface proteins across NK cell immune synapses, TRAFFIC, Vol: 8, Pages: 1190-1204, ISSN: 1398-9219
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- Citations: 38
Endt J, McCann FE, Almeida CR, et al., 2007, Inhibitory receptor signals suppress ligation-induced recruitment of NKG2D to GM1-Rich membrane domains at the human NK cell immune synapse, JOURNAL OF IMMUNOLOGY, Vol: 178, Pages: 5606-5611, ISSN: 0022-1767
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- Citations: 42
Nedvetzki S, Sowinski S, Eagle RA, et al., 2007, Reciprocal regulation of human natural killer cells and macrophages associated with distinct immune synapses, BLOOD, Vol: 109, Pages: 3776-3785, ISSN: 0006-4971
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- Citations: 200
McCann FE, Eissmann P, Onfelt B, et al., 2007, The activating NKG2D ligand MHC class I-related chain a transfers from target cells to NK cells in a manner that allows functional consequences, JOURNAL OF IMMUNOLOGY, Vol: 178, Pages: 3418-3426, ISSN: 0022-1767
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- Citations: 60
Gehring AJ, Sun D, Kennedy PTF, et al., 2007, The level of viral antigen presented by hepatocytes influences CD8 T-cell function, JOURNAL OF VIROLOGY, Vol: 81, Pages: 2940-2949, ISSN: 0022-538X
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- Citations: 68
Davis DM, 2007, Intercellular transfer of cell-surface proteins is common and can affect many stages of an immune response, NATURE REVIEWS IMMUNOLOGY, Vol: 7, Pages: 238-243, ISSN: 1474-1733
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- Citations: 210
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