Imperial College London
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ProfessorDanielDavis

Faculty of Natural Sciences, Department of Life Sciences

Head of Department of Life Sciences, Chair in Immunology
 
 
 
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Contact

 

+44 (0)20 7594 5420d.davis CV

 
 
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Location

 

609Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Oszmiana:2016:10.1016/j.celrep.2016.04.075,
author = {Oszmiana, A and Williamson, DJ and Cordoba, SP and Morgan, DJ and Kennedy, PR and Stacey, K and Davis, DM},
doi = {10.1016/j.celrep.2016.04.075},
journal = {Cell Reports},
pages = {1957--1972},
title = {The Size of Activating and Inhibitory Killer Ig-like Receptor Nanoclusters Is Controlled by the Transmembrane Sequence and Affects Signaling},
url = {http://dx.doi.org/10.1016/j.celrep.2016.04.075},
volume = {15},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Super-resolution microscopy has revealed that immune cell receptors are organized in nanoscale clusters at cell surfaces and immune synapses. However, mechanisms and functions for this nanoscale organization remain unclear. Here, we used super-resolution microscopy to compare the surface organization of paired killer Ig-like receptors (KIR), KIR2DL1 and KIR2DS1, on human primary natural killer cells and cell lines. Activating KIR2DS1 assembled in clusters two-fold larger than its inhibitory counterpart KIR2DL1. Site-directed mutagenesis established that the size of nanoclusters is controlled by transmembrane amino acid 233, a lysine in KIR2DS1. Super-resolution microscopy also revealed two ways in which the nanoscale clustering of KIR affects signaling. First, KIR2DS1 and DAP12 nanoclusters are juxtaposed in the resting cell state but coalesce upon receptor ligation. Second, quantitative super-resolution microscopy revealed that phosphorylation of the kinase ZAP-70 or phosphatase SHP-1 is favored in larger KIR nanoclusters. Thus, the size of KIR nanoclusters depends on the transmembrane sequence and affects downstream signaling.
AU  - Oszmiana,A
AU  - Williamson,DJ
AU  - Cordoba,SP
AU  - Morgan,DJ
AU  - Kennedy,PR
AU  - Stacey,K
AU  - Davis,DM
DO  - 10.1016/j.celrep.2016.04.075
EP  - 1972
PY  - 2016///
SN  - 2211-1247
SP  - 1957
TI  - The Size of Activating and Inhibitory Killer Ig-like Receptor Nanoclusters Is Controlled by the Transmembrane Sequence and Affects Signaling
T2  - Cell Reports
UR  - http://dx.doi.org/10.1016/j.celrep.2016.04.075
UR  - http://hdl.handle.net/10044/1/33049
VL  - 15
ER  -
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