Imperial College London
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ProfessorDenisDoorly

Faculty of Engineering, Department of Aeronautics

Professor of Fluid Mechanics
 
 
 
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Contact

 

d.doorly

 
 
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Location

 

313ACity and Guilds BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Scott:2022:10.1002/nbm.4692,
author = {Scott, A and Jackson, T and Khalique, Z and Gorodezky, M and Pardoe, B and Begum, L and Bruno, VD and Chowdhury, R and Ferreira, P and Nielles-Vallespin, S and Roehl, M and McCarthy, K and Sarathchandra, P and Rose, J and Doorly, D and Pennell, D and Ascione, R and De, Silva PER and Firmin, D},
doi = {10.1002/nbm.4692},
journal = {NMR in Biomedicine},
title = {Development of a CMR compatible large animal isolated heart model for direct comparison of beating and arrested hearts},
url = {http://dx.doi.org/10.1002/nbm.4692},
volume = {35},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundCardiac motion results in image artefacts and quantification errors in many cardiovascular magnetic resonance (CMR) techniques, including microstructural assessment using diffusion tensor cardiovascular magnetic resonance (DT-CMR). Here we develop a CMR compatible isolated perfused porcine heart model that allows comparison of data obtained in beating and arrested states.Methods10 porcine hearts (8/10 for protocol optimisation) were harvested using a donor heart retrieval protocol and transported to the remote CMR facility. Langendorff perfusion in a 3D printed chamber and perfusion circuit re-established contraction. Hearts were imaged using cine, parametric mapping and STEAM DT-CMR at cardiac phases with the minimum and maximum wall thickness. High potassium and lithium perfusates were then used to arrest the heart in a slack and contracted state respectively. Imaging was repeated in both arrested states. After imaging, tissue was removed for subsequent histology in a location matched to the DT-CMR data using fiducial markers.ResultsRegular sustained contraction was successfully established in 6/10 hearts, including the final 5 hearts. Imaging was performed in 4 hearts and one underwent the full protocol including co-localised histology. Image quality was good and there was good agreement between DT-CMR data in equivalent beating and arrested states. Despite the use of autologous blood and dextran within the perfusate, T2, DT-CMR measures and an increase in mass was consistent with development of myocardial edema resulting in failure to achieve a true diastolic-like state. A contiguous stack of 313 5μm histological sections at and a 100μm thick section showing cell morphology on 3D fluorescent confocal microscopy co-localised to DT-CMR data were obtained.ConclusionsA CMR compatible isolated perfused beating heart setup for large animal hearts allows direct comparisons of beating and arrested heart data with subsequent co-localised histology without
AU  - Scott,A
AU  - Jackson,T
AU  - Khalique,Z
AU  - Gorodezky,M
AU  - Pardoe,B
AU  - Begum,L
AU  - Bruno,VD
AU  - Chowdhury,R
AU  - Ferreira,P
AU  - Nielles-Vallespin,S
AU  - Roehl,M
AU  - McCarthy,K
AU  - Sarathchandra,P
AU  - Rose,J
AU  - Doorly,D
AU  - Pennell,D
AU  - Ascione,R
AU  - De,Silva PER
AU  - Firmin,D
DO  - 10.1002/nbm.4692
PY  - 2022///
SN  - 0952-3480
TI  - Development of a CMR compatible large animal isolated heart model for direct comparison of beating and arrested hearts
T2  - NMR in Biomedicine
UR  - http://dx.doi.org/10.1002/nbm.4692
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/nbm.4692
UR  - http://hdl.handle.net/10044/1/94262
VL  - 35
ER  -
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