Imperial College London
Alternate

ProfessorDavidHolden

Faculty of Medicine, Department of Infectious Disease

Professor
 
 
 
//

Contact

 

+44 (0)20 7594 3073d.holden

 
 
//

Location

 

221Flowers buildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Cerny:2021:10.1371/journal.ppat.1009771,
author = {Cerny, O and Godlee, C and Tocci, R and Cross, NE and Shi, H and Williamson, JC and Alix, E and Lehner, PJ and Holden, DW},
doi = {10.1371/journal.ppat.1009771},
journal = {PLoS Pathogens},
pages = {1--28},
title = {CD97 stabilises the immunological synapse between dendritic cells and T cells and is targeted for degradation by the Salmonella effector SteD},
url = {http://dx.doi.org/10.1371/journal.ppat.1009771},
volume = {17},
year = {2021}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The Salmonella enterica effector SteD depletes mature MHC class II (mMHCII) molecules from the surface of infected antigen-presenting cells through ubiquitination of the cytoplasmic tail of the mMHCII β chain. This requires the Nedd4 family HECT E3 ubiquitin ligase Wwp2 and a tumor-suppressing transmembrane protein adaptor Tmem127. Here, through a proteomic screen of dendritic cells, we found that SteD targets the plasma membrane protein CD97 for degradation by a similar mechanism. SteD enhanced ubiquitination of CD97 on K555 and mutation of this residue eliminated the effect of SteD on CD97 surface levels. We showed that CD97 localises to and stabilises the immunological synapse between dendritic cells and T cells. Removal of CD97 by SteD inhibited dendritic cell-T cell interactions and reduced T cell activation, independently of its effect on MHCII. Therefore, SteD suppresses T cell immunity by two distinct processes.
AU  - Cerny,O
AU  - Godlee,C
AU  - Tocci,R
AU  - Cross,NE
AU  - Shi,H
AU  - Williamson,JC
AU  - Alix,E
AU  - Lehner,PJ
AU  - Holden,DW
DO  - 10.1371/journal.ppat.1009771
EP  - 28
PY  - 2021///
SN  - 1553-7366
SP  - 1
TI  - CD97 stabilises the immunological synapse between dendritic cells and T cells and is targeted for degradation by the Salmonella effector SteD
T2  - PLoS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1009771
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000677859100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009771
UR  - http://hdl.handle.net/10044/1/90894
VL  - 17
ER  -
Download