Imperial College London
Alternate

ProfessorDavidHolden

Faculty of Medicine, Department of Infectious Disease

Professor
 
 
 
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Contact

 

+44 (0)20 7594 3073d.holden

 
 
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Location

 

221Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rolhion:2016:10.1371/journal.ppat.1005653,
author = {Rolhion, N and Furniss, R and Grabe, G and Ryan, A and Liu, M and Matthews, S and Holden, DW},
doi = {10.1371/journal.ppat.1005653},
journal = {Plos Pathogens},
pages = {1--26},
title = {Inhibition of nuclear transport of NF-kB p65 by the Salmonella type III secretion system effector SpvD},
url = {http://dx.doi.org/10.1371/journal.ppat.1005653},
volume = {12},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Salmonella enterica replicates in macrophages through the action of effector proteins translocated across the vacuolar membrane by a type III secretion system (T3SS). Here we show that the SPI-2 T3SS effector SpvD suppresses proinflammatory immune responses. SpvD prevented activation of an NF-B-dependent promoter and caused nuclear accumulation of importin-α, which is required for nuclear import of p65. SpvD interacted specifically with the exportin Xpo2, which mediates nuclear-cytoplasmic recycling of importins. We propose that interaction between SpvD and Xpo2 disrupts the normal recycling of importin-α from the nucleus, leading to a defect in nuclear translocation of p65 and inhibition of activation of NF-B regulated promoters. SpvD down-regulated pro-inflammatory responses and contributed to systemic growth of bacteria in mice. This work shows that a bacterial pathogen can manipulate host cell immune responses by interfering with the nuclear transport machinery.
AU  - Rolhion,N
AU  - Furniss,R
AU  - Grabe,G
AU  - Ryan,A
AU  - Liu,M
AU  - Matthews,S
AU  - Holden,DW
DO  - 10.1371/journal.ppat.1005653
EP  - 26
PY  - 2016///
SN  - 1553-7374
SP  - 1
TI  - Inhibition of nuclear transport of NF-kB p65 by the Salmonella type III secretion system effector SpvD
T2  - Plos Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1005653
UR  - https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1005653
UR  - http://hdl.handle.net/10044/1/32716
VL  - 12
ER  -
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