Imperial College London
Alternate

ProfessorDavidHolden

Faculty of Medicine, Department of Infectious Disease

Professor
 
 
 
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Contact

 

+44 (0)20 7594 3073d.holden

 
 
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Location

 

221Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Thurston:2016:10.1038/ncomms13292,
author = {Thurston, T and Matthews, S and Jennings, E and Alix, E and Shao, F and Shenoy, A and Birrell, M and Holden, D},
doi = {10.1038/ncomms13292},
journal = {Nature Communications},
title = {Growth inhibition of cytosolic Salmonella by caspase-1 and caspase-11 precedes host cell death},
url = {http://dx.doi.org/10.1038/ncomms13292},
volume = {7},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Sensing bacterial products in the cytosol of mammalian cells by NOD-like receptors leads to the activation of caspase-1 inflammasomes, and the production of the pro-inflammatory cytokines interleukin (IL)-18 and IL-1β. In addition, mouse caspase-11 (represented in humans by its orthologs, caspase-4 and caspase-5) detects cytosolic bacterial LPS directly. Activation of caspase-1 and caspase-11 initiates pyroptotic host cell death that releases potentially harmful bacteria from the nutrient-rich host cell cytosol into the extracellular environment. Here we use single cell analysis and time-lapse microscopy to identify a subpopulation of host cells, in which growth of cytosolic Salmonella Typhimurium is inhibited independently or prior to the onset of cell death. The enzymatic activities of caspase-1 and caspase-11 are required for growth inhibition in different cell types. Our results reveal that these proteases have important functions beyond the direct induction of pyroptosis and proinflammatory cytokine secretion in the control of growth and elimination of cytosolic bacteria.
AU  - Thurston,T
AU  - Matthews,S
AU  - Jennings,E
AU  - Alix,E
AU  - Shao,F
AU  - Shenoy,A
AU  - Birrell,M
AU  - Holden,D
DO  - 10.1038/ncomms13292
PY  - 2016///
SN  - 2041-1723
TI  - Growth inhibition of cytosolic Salmonella by caspase-1 and caspase-11 precedes host cell death
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms13292
UR  - http://hdl.handle.net/10044/1/42306
VL  - 7
ER  -
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