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@article{Davis:2022:10.1101/2022.10.18.22280274,
author = {Davis, EL and Hollingsworth, TD},
doi = {10.1101/2022.10.18.22280274},
title = {Estimating LFT and qPCR test-sensitivity over time since infection from a human challenge study},
url = {http://dx.doi.org/10.1101/2022.10.18.22280274},
year = {2022}
}

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TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>Testing has been central to global policy throughout the SARS-CoV-2 pandemic. Understanding how test sensitivity changes after exposure is crucial for the interpretation of test outcomes and the design of testing-based interventions. Using data from a human challenge study, we derive temporal test sensitivity profiles for lateral flow tests (LFT), quantitative polymerase chain reaction (qPCR) tests and viable virus, measured by focus-forming assay (FFA). The median time to detectability was 2 days (throat swab) and 3 days (nasal) for qPCR and 4 days for LFT (both swabs), and there was strong positive correlation between first LFT positive and first FFA positive for both throat (p=0.00019) and nasal (p=0.00032) swabs, supporting the use of LFTs as a method of detecting infectiousness. Peak LFT sensitivity was 82.4% (67.0%-91.8%) for throat samples, occurring 6 days post-exposure and 93.3% (85.1%-98.0%) for nasal, 7 days post-exposure. These temporal profiles provide quantification of the mean behaviour of these tests and individual-based variability and could inform a framework for investigating future testing-based interventions.</jats:p>
AU  - Davis,EL
AU  - Hollingsworth,TD
DO  - 10.1101/2022.10.18.22280274
PY  - 2022///
TI  - Estimating LFT and qPCR test-sensitivity over time since infection from a human challenge study
UR  - http://dx.doi.org/10.1101/2022.10.18.22280274
ER  -

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Professor Deirdre Hollingsworth

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