Imperial College London

ProfessorDavidKlug

Faculty of Natural SciencesDepartment of Chemistry

Professor
 
 
 
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Contact

 

+44 (0)20 7594 5806d.klug

 
 
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Location

 

267ChemistrySouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

167 results found

Tahirbegi B, Magness AJ, Boillat A, Willison KR, Klug DR, Knopfel T, Ying Let al., 2018, Probing Synaptic Amyloid-Beta Aggregation Promoted by Copper Release, 62nd Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 430A-430A, ISSN: 0006-3495

CONFERENCE PAPER

Cilibrizzi A, Terenghi M, Fedorova M, Woscholski R, Klug D, Vilar Ret al., 2017, Small-molecule optical probes for cell imaging of protein sulfenylation and their application to monitor cisplatin induced protein oxidation, SENSORS AND ACTUATORS B-CHEMICAL, Vol: 248, Pages: 437-446, ISSN: 0925-4005

JOURNAL ARTICLE

Sim S, Sowley H, Kidley N, Barter L, Klug Det al., 2017, Investigation of inhibitor-protein interactions in plants & mammalians from EVV 2DIR data, 254th National Meeting and Exposition of the American-Chemical-Society (ACS) on Chemistry's Impact on the Global Economy, Publisher: AMER CHEMICAL SOC, ISSN: 0065-7727

CONFERENCE PAPER

Magness AJ, Squires JA, Griffiths B, Khan K, Swain A, Willison KR, Cunningham D, Gerlinger M, Klug DRet al., 2017, Multiplexed single cell protein expression analysis in solid tumours using a miniaturised microfluidic assay, CONVERGENT SCIENCE PHYSICAL ONCOLOGY, Vol: 3, ISSN: 2057-1739

JOURNAL ARTICLE

Willison KR, Salehi-Reyhani A, Burgin E, Barclay M, Brown A, Neil MA, Ces O, Klug DRet al., 2015, Absolute quantification of protein copy number in single cells using single molecule microarrays, EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol: 44, Pages: S179-S179, ISSN: 0175-7571

JOURNAL ARTICLE

Salehi-Reyhani A, Gesellchen F, Mampallil D, Wilson R, Reboud J, Ces O, Willison KR, Cooper JM, Klug DRet al., 2015, Chemical-Free Lysis and Fractionation of Cells by Use of Surface Acoustic Waves for Sensitive Protein Assays, ANALYTICAL CHEMISTRY, Vol: 87, Pages: 2161-2169, ISSN: 0003-2700

JOURNAL ARTICLE

Mampallil D, Reboud J, Wilson R, Wylie D, Klug DR, Cooper JMet al., 2015, Acoustic suppression of the coffee-ring effect, SOFT MATTER, Vol: 11, Pages: 7207-7213, ISSN: 1744-683X

JOURNAL ARTICLE

Forster M, Potter RJ, Ling Y, Yang Y, Klug DR, Li Y, Cowan AJet al., 2015, Oxygen deficient alpha-Fe2O3 photoelectrodes: a balance between enhanced electrical properties and trap-mediated losses, CHEMICAL SCIENCE, Vol: 6, Pages: 4009-4016, ISSN: 2041-6520

JOURNAL ARTICLE

Casey DR, Wylie D, Gallo J, Dent MR, Salehi-Reyhani A, Wilson R, Brooks NJ, Long NJ, Willison KR, Klug DR, Neil MAA, Neale SL, Cooper JM, Ces Oet al., 2015, A Novel, all-optical tool for controllable and non-destructive poration of cells with single-micron resolution

© OSA 2015. We demonstrate controllable poration within ≈1 μm regions of individual cells, mediated by a near-IR laser interacting with thin-layer amorphous silicon substrates. This technique will allow new experiments in single-cell biology, particularly in neuroscience. As our understanding of the fundamental mechanistic processes underpinning biology expands, so does the need for high-precision tools to allow the dissection of the heterogeneity and stochastic processes that dominate at the single- and sub-cellular level. Here, we demonstrate a highly controllable and reproducible optical technique for inducing poration within specific regions of a target cell's plasma membrane, permitting localized delivery of payloads, depolarization and lysis experiments to be conducted in unprecedented detail. Experiments support a novel mechanism for the process, based upon a thermally-induced change triggered by the interactions of a near-IR laser with a biocompatible thin film substrate at powers substantially below that used in standard optoporation experiments.

CONFERENCE PAPER

Valim LR, Davies JA, Jensen KT, Guo R, Willison KR, Spickett CM, Pitt AR, Klug DRet al., 2014, Identification and Relative Quantification of Tyrosine Nitration in a Model Peptide Using Two-Dimensional Infrared Spectroscopy, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 118, Pages: 12855-12864, ISSN: 1520-6106

JOURNAL ARTICLE

Schrems A, Phillips J, Casey D, Wylie D, Novakova M, Sleytr UB, Klug DR, Neil MAA, Schuster B, Ces Oet al., 2014, The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells (vol 139, pg 3296, 2014), ANALYST, Vol: 139, Pages: 4382-4382, ISSN: 0003-2654

JOURNAL ARTICLE

Schrems A, Phillips J, Casey D, Wylie D, Novakova M, Sleytr UB, Klug D, Neil MAA, Schuster B, Ces Oet al., 2014, Erratum: The grab-and-drop protocol: A novel strategy for membrane protein isolation and reconstitution from single cells (Analyst (2014) DOI: 10.1039/C4AN00059E), Analyst, Vol: 139, ISSN: 0003-2654

JOURNAL ARTICLE

Salehi-Reyhani A, Burgin E, Ces O, Willison KR, Klug DRet al., 2014, Addressable droplet microarrays for single cell protein analysis, ANALYST, Vol: 139, Pages: 5367-5374, ISSN: 0003-2654

JOURNAL ARTICLE

Schrems A, Phillips J, Casey D, Wylie D, Novakova M, Sleytr UB, Klug D, Neil MAA, Schuster B, Ces Oet al., 2014, The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells, ANALYST, Vol: 139, Pages: 3296-3304, ISSN: 0003-2654

JOURNAL ARTICLE

Burgin E, Salehi-Reyhani A, Barclay M, Brown A, Kaplinsky J, Novakova M, Neil MAA, Ces O, Willison KR, Klug DRet al., 2014, Absolute quantification of protein copy number using a single-molecule-sensitive microarray, ANALYST, Vol: 139, Pages: 3235-3244, ISSN: 0003-2654

JOURNAL ARTICLE

Pastor E, Pesci FM, Reynal A, Handoko AD, Guo M, An X, Cowan AJ, Klug DR, Durrant JR, Tang Jet al., 2014, Interfacial charge separation in Cu2O/RuOx as a visible light driven CO2 reduction catalyst, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 16, Pages: 5922-5926, ISSN: 1463-9076

JOURNAL ARTICLE

Salehi-Reyhani A, Sharma S, Burgin E, Barclay M, Cass A, Neil MAA, Ces O, Willison KR, Klug DR, Brown A, Novakova Met al., 2014, Scaling advantages and constraints in miniaturized capture assays for single cell protein analysis (vol 13, pg 2066, 2013), LAB ON A CHIP, Vol: 14, Pages: 3430-3430, ISSN: 1473-0197

JOURNAL ARTICLE

Pesci FM, Wang G, Klug DR, Li Y, Cowan AJet al., 2013, Efficient Suppression of Electron Hole Recombination in Oxygen-Deficient Hydrogen-Treated TiO2 Nanowires for Photoelectrochemical Water Splitting, JOURNAL OF PHYSICAL CHEMISTRY C, Vol: 117, Pages: 25837-25844, ISSN: 1932-7447

JOURNAL ARTICLE

Willison KR, Klug DR, 2013, Quantitative single cell and single molecule proteomics for clinical studies, CURRENT OPINION IN BIOTECHNOLOGY, Vol: 24, Pages: 745-751, ISSN: 0958-1669

JOURNAL ARTICLE

Salehi-Reyhani A, Sharma S, Burgin E, Barclay M, Cass A, Neil MAA, Ces O, Willison KR, Klug DRet al., 2013, Scaling advantages and constraints in miniaturized capture assays for single cell protein analysis, LAB ON A CHIP, Vol: 13, Pages: 2066-2074, ISSN: 1473-0197

JOURNAL ARTICLE

Cowan AJ, Leng W, Barnes PRF, Klug DR, Durrant JRet al., 2013, Charge carrier separation in nanostructured TiO2 photoelectrodes for water splitting, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 15, Pages: 8772-8778, ISSN: 1463-9076

JOURNAL ARTICLE

Barroso M, Mesa CA, Pendlebury SR, Cowan AJ, Hisatomi T, Sivula K, Graetzel M, Klug DR, Durrant JRet al., 2012, Dynamics of photogenerated holes in surface modified alpha-Fe2O3 photoanodes for solar water splitting, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 109, Pages: 15640-15645, ISSN: 0027-8424

JOURNAL ARTICLE

Gierakowski L, Guo R, Kornau KM, Fournier F, Davies JA, Klug Det al., 2012, Study of protein-tyrosine nitration by two-dimensional infrared vibrational spectroscopy, FREE RADICAL BIOLOGY AND MEDICINE, Vol: 53, Pages: S214-S214, ISSN: 0891-5849

JOURNAL ARTICLE

Wylie D, Casey D, Phillips J, Klug D, Ces O, Neil Met al., 2012, Novel nanotechnologies for multiple spatially and temporally resolved live single cell membrane sampling and analysis, FREE RADICAL BIOLOGY AND MEDICINE, Vol: 53, Pages: S127-S128, ISSN: 0891-5849

JOURNAL ARTICLE

Salehi-Reyhani A, Barclay M, Ces O, Willison K, Klug Det al., 2012, Towards Practical Single Cell Proteomics: A Microfluidic Antibody Capture Chip with TIRF Detection, FREE RADICAL BIOLOGY AND MEDICINE, Vol: 53, Pages: S128-S128, ISSN: 0891-5849

JOURNAL ARTICLE

Lanigan PMP, Munro I, Grace EJ, Casey DR, Phillips J, Klug DR, Ces O, Neil MAAet al., 2012, Dynamical hologram generation for high speed optical trapping of smart droplet microtools, BIOMEDICAL OPTICS EXPRESS, Vol: 3, Pages: 1609-1619, ISSN: 2156-7085

JOURNAL ARTICLE

Goyder MS, Willison KR, Klug DR, deMello AJ, Ces Oet al., 2012, Affinity chromatography and capillary electrophoresis for analysis of the yeast ribosomal proteins, BMB REPORTS, Vol: 45, Pages: 233-238, ISSN: 1976-6696

JOURNAL ARTICLE

Pendlebury SR, Cowan AJ, Barroso M, Sivula K, Ye J, Graetzel M, Klug DR, Tang J, Durrant JRet al., 2012, Correlating long-lived photogenerated hole populations with photocurrent densities in hematite water oxidation photoanodes, ENERGY & ENVIRONMENTAL SCIENCE, Vol: 5, Pages: 6304-6312, ISSN: 1754-5692

JOURNAL ARTICLE

Guo R, Mukamel S, Klug DR, 2012, Geometry determination of complexes in a molecular liquid mixture using electron-vibration-vibration two-dimensional infrared spectroscopy with a vibrational transition density cube method, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 14, Pages: 14023-14033, ISSN: 1463-9076

JOURNAL ARTICLE

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