Publications
196 results found
Schrems A, Phillips J, Casey D, et al., 2014, The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells, ANALYST, Vol: 139, Pages: 3296-3304, ISSN: 0003-2654
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- Citations: 8
Pastor E, Pesci FM, Reynal A, et al., 2014, Interfacial charge separation in Cu2O/RuOx as a visible light driven CO2 reduction catalyst, Physical Chemistry Chemical Physics, ISSN: 1463-9076
We employ transient absorption spectroscopy to record the absorption spectrum of photogenerated charge carriers in Cu2O. We have found that CO2 reduction in Cu2O is limited by fast electron-hole recombination. The deposition of RuOx nanoparticles on Cu2O results in a twofold increased yield of long-lived electrons, indicating partially reduced electron-hole recombination losses. This observation correlates with an approximately sixfold increase in the yield of CO2 reduction to CO.
Salehi-Reyhani A, Sharma S, Burgin E, et al., 2014, Scaling advantages and constraints in miniaturized capture assays for single cell protein analysis (vol 13, pg 2066, 2013), LAB ON A CHIP, Vol: 14, Pages: 3430-3430, ISSN: 1473-0197
Pesci FM, Wang G, Klug DR, et al., 2013, Efficient suppression of electron hole recombination in oxygen-deficient hydrogen-treated TiO2 nanowires for photoelectrochemical water splitting, The Journal of Physical Chemistry C: Energy Conversion and Storage, Optical and Electronic Devices, Interfaces, Nanomaterials, and Hard Matter, Vol: 117, Pages: 25837-25844, ISSN: 1932-7447
There is an increasing level of interest in the use of black TiO2 prepared by thermal hydrogen treatments (H:TiO2) due to the potential to enhance both the photocatalytic and the light-harvesting properties of TiO2. Here, we examine oxygen-deficient H:TiO2 nanotube arrays that have previously achieved very high solar-to-hydrogen (STH) efficiencies due to incident photon-to-current efficiency (IPCE) values of >90% for photoelectrochemical water splitting at only 0.4 V vs RHE under UV illumination. Our transient absorption (TA) mechanistic study provides strong evidence that the improved electrical properties of oxygen-deficient TiO2 enables remarkably efficient spatial separation of electron–hole pairs on the submicrosecond time scale at moderate applied bias, and this coupled to effective suppression of microsecond to seconds charge carrier recombination is the primary factor behind the dramatically improved photoelectrochemical activity.
Willison KR, Klug DR, 2013, Quantitative single cell and single molecule proteomics for clinical studies, CURRENT OPINION IN BIOTECHNOLOGY, Vol: 24, Pages: 745-751, ISSN: 0958-1669
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- Citations: 27
Durrant JR, Nelson J, Klug DR, 2013, Functionalising nanocrystalline TiO<sub>2</sub> films:: dye sensitised solar cells and optical biosensors, Materials Congress 2000, Publisher: TAYLOR & FRANCIS LTD, Pages: 1345-1348, ISSN: 0267-0836
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- Citations: 16
Salehi-Reyhani A, Sharma S, Burgin E, et al., 2013, Scaling Advantages and Constraints in Miniaturized Capture Assays for Single Cell Protein Analysis, Lab on A Chip, Vol: 13, Pages: 2066-2074, ISSN: 1473-0197
Measuring protein expression in single cells is the basis of single cell proteomics. The sensitivity and dynamic range of a single cell immunoassay should ideally be such that proteins that are expressed between 1 – 106 copies per cell can be detected and counted. We have investigated the effect of miniaturizing antibody microarrays by reducing capture spot sizes from 100 μm to 15 μm using dip pen nanolithography. We demonstrate that protocols developed for printing and passivating antibody capture spots using conventional pin based contact printing can be directly transferred to dip-pen lithography whilst retaining the capture activity per unit area. Using a simple kinetic model, we highlight how the limit of detection and dynamic range of a sandwich immunoassay, respectively, increase and decrease when spot size is reduced. However, we show that reducing spot size is more effective than increasing assay chamber volume when seeking to multiplex such a microfluidic immunoassay. Although, we make particular reference to single cell microfluidic immunoassays, the topics discussed here are applicable to capture assays in general.
Cowan AJ, Leng W, Barnes PRF, et al., 2013, Charge carrier separation in nanostructured TiO<sub>2</sub> photoelectrodes for water splitting, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 15, Pages: 8772-8778, ISSN: 1463-9076
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- Citations: 58
Barroso M, Mesa CA, Pendlebury SR, et al., 2012, Dynamics of photogenerated holes in surface modified α-Fe<sub>2</sub>O<sub>3</sub> photoanodes for solar water splitting, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 109, Pages: 15640-15645, ISSN: 0027-8424
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- Citations: 380
Gierakowski L, Guo R, Kornau KM, et al., 2012, Study of protein-tyrosine nitration by two-dimensional infrared vibrational spectroscopy, FREE RADICAL BIOLOGY AND MEDICINE, Vol: 53, Pages: S214-S214, ISSN: 0891-5849
Wylie D, Casey D, Phillips J, et al., 2012, Novel nanotechnologies for multiple spatially and temporally resolved live single cell membrane sampling and analysis, FREE RADICAL BIOLOGY AND MEDICINE, Vol: 53, Pages: S127-S128, ISSN: 0891-5849
Salehi-Reyhani A, Barclay M, Ces O, et al., 2012, Towards Practical Single Cell Proteomics: A Microfluidic Antibody Capture Chip with TIRF Detection, FREE RADICAL BIOLOGY AND MEDICINE, Vol: 53, Pages: S128-S128, ISSN: 0891-5849
Goyder MS, Willison KR, Klug DR, et al., 2012, Affinity chromatography and capillary electrophoresis for analysis of the yeast ribosomal proteins, BMB REPORTS, Vol: 45, Pages: 233-238, ISSN: 1976-6696
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- Citations: 3
Guo R, Mukamel S, Klug DR, 2012, Geometry determination of complexes in a molecular liquid mixture using electron-vibration-vibration two-dimensional infrared spectroscopy with a vibrational transition density cube method, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 14, Pages: 14023-14033, ISSN: 1463-9076
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- Citations: 11
Pendlebury SR, Cowan AJ, Barroso M, et al., 2012, Correlating long-lived photogenerated hole populations with photocurrent densities in hematite water oxidation photoanod, Energy and Environmental Science
Lanigan PMP, Munro I, Grace EJ, et al., 2012, Dynamical hologram generation for high speed optical trapping of smart droplet microtools, Biomedical Optics Express, Vol: 3, Pages: 1609-1609
Barroso M, Cowan AJ, Pendlebury SR, et al., 2011, The Role of Cobalt Phosphate in Enhancing the Photocatalytic Activity of alpha-Fe(2)O(3) toward Water Oxidation, ISSN: 0002-7863
Pesci FM, Cowan AJ, Alexander BD, et al., 2011, Charge Carrier Dynamics on Mesoporous WO<sub>3</sub> during Water Splitting, JOURNAL OF PHYSICAL CHEMISTRY LETTERS, Vol: 2, Pages: 1900-1903, ISSN: 1948-7185
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- Citations: 138
Cowan AJ, Barnett CJ, Pendlebury SR, et al., 2011, Activation Energies for the Rate-Limiting Step in Water Photooxidation by Nanostructured alpha-Fe(2)O(3) and TiO(2), ISSN: 0002-7863
Tang J, Cowan AJ, Durrant JR, et al., 2011, Mechanism of O<sub>2</sub> Production from Water Splitting: Nature of Charge Carriers in Nitrogen Doped Nanocrystalline TiO<sub>2</sub> Films and Factors Limiting O<sub>2</sub> Production, JOURNAL OF PHYSICAL CHEMISTRY C, Vol: 115, Pages: 3143-3150, ISSN: 1932-7447
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- Citations: 121
Salehi-Reyhani A, Kaplinsky J, Burgin E, et al., 2011, A first step towards practical single cell proteomics: a microfluidic antibodycapture chip with TIRF detection, Lab Chip, Vol: 11, Pages: 1256-1261
We have developed a generic platform to undertake the analysis of protein copy number from singlecells. The approach described here is ‘all-optical’ whereby single cells are manipulated into separate analysis chambers using an optical trap; single cells are lysed by a shock wave caused by laser-induced microcavitation, and the protein released from a single cell is measured by total internal reflection microscopy as it is bound to micro-printed antibody spots within the device. The platform was tested using GFP transfected cells and the relative precision of the measurement method was determined to be 88%. Single cell measurements were also made on a breast cancer cell line to measure the relative levels of unlabelled human tumour suppressor protein p53 using a chip incorporating an antibody sandwich assay format. These results suggest that this is a viable method for measuring relative protein levels in single cells.
Cowan AJ, Pendlebury SR, Barroso M, et al., 2011, Charge carrier dynamics in metal oxide water splitting photoelectrodes, 242nd ACS National Meeting
Guo R, Miele M, Gardner EM, et al., 2011, Potential for the detection of molecular complexes and determination of interaction geometry by 2DIR: Application to protein sciences, FARADAY DISCUSSIONS, Vol: 150, Pages: 161-174, ISSN: 1359-6640
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- Citations: 6
Pendlebury SR, Barroso M, Cowan AJ, et al., 2010, Dynamics of photogenerated holes in nanocrystalline alpha-Fe(2)O(3) electrodes for water oxidation probed by transient absorption spectroscopy, ISSN: 1359-7345
Donaldson PM, Willison KR, Klug DR, 2010, Generation of Simplified Protein Raman Spectra Using Three-Color Picosecond Coherent Anti-Stokes Raman Spectroscopy, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 114, Pages: 12175-12181, ISSN: 1520-6106
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- Citations: 4
Gould IR, Guo R, Donaldson PM, et al., 2010, Detection of complex formation and determination of intermolecular geometry through electrical anharmonic coupling of molecular vibrations using electron-vibration-vibration 2D infrared spectroscopy, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol: 239, ISSN: 0065-7727
Cowan AJ, Tang J, Leng W, et al., 2010, Water Splitting by Nanocrystalline TiO<sub>2</sub> in a Complete Photoelectrochemical Cell Exhibits Efficiencies Limited by Charge Recombination, JOURNAL OF PHYSICAL CHEMISTRY C, Vol: 114, Pages: 4208-4214, ISSN: 1932-7447
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- Citations: 212
Loeffeld C, Barnett CJ, Kaplinsky J, et al., 2010, Optical Proteomics Combining Nonlinear Electrokinetics and Coherent Two-Dimensional Infrared Spectroscopy, BIOPHYSICAL JOURNAL, Vol: 98, Pages: 17A-17A, ISSN: 0006-3495
Klug DR, Guo R, Fournier F, et al., 2010, Detection of Molecular Complex Formation and Direct Determination of Intermolecular Interaction Geometries by a Hybrid Raman-Infrared Multidimensional Coherent Spectroscopy: Implications for High Throughput Biology, 22nd International Conference on Raman Spectroscopy, Publisher: AMER INST PHYSICS, Pages: 113-+, ISSN: 0094-243X
Fournier F, Guo R, Gardner EM, et al., 2009, Biological and Biomedical Applications of Two-Dimensional Vibrational Spectroscopy: Proteomics, Imaging, and Structural Analysis, ACCOUNTS OF CHEMICAL RESEARCH, Vol: 42, Pages: 1322-1331, ISSN: 0001-4842
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- Citations: 44
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