Imperial College London
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ProfessorDavidKlug

Faculty of Natural Sciences, Department of Chemistry

Associate Dean for Enterprise for FoNS & Professor
 
 
 
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Contact

 

+44 (0)20 7594 5806d.klug

 
 
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Location

 

Molecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@inproceedings{Mickleburgh:2020,
author = {Mickleburgh, TG and Salehi-Reyhani, A and Magness, AJ and Joyce, WD and Ces, O and Klug, DR},
pages = {183--184},
title = {A miniaturized microfluidic assay for single plant cell protein quantitation},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - CPAPER
AB  - We present a method based on a miniaturized antibody capture chip to perform protein quantitation in plants with single cell single molecule resolution. We demonstrate the versatility of the method in capturing and analyzing membrane, cytosolic, and chloroplast stroma located proteins, specifically demonstrated by SNARE 12: YFP, 35S: GFP, and Ribulose-1, 5-bisphosphate carboxylase/oxygenase, respectively. Single cell protein expression quantification is achieved through a bi or tri-molecular antibody microarray. We have also reported single cell experimental evidence that a reduction in chamber volume enhances assay sensitivity.
AU  - Mickleburgh,TG
AU  - Salehi-Reyhani,A
AU  - Magness,AJ
AU  - Joyce,WD
AU  - Ces,O
AU  - Klug,DR
EP  - 184
PY  - 2020///
SP  - 183
TI  - A miniaturized microfluidic assay for single plant cell protein quantitation
ER  -
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