Imperial College London
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ProfessorDavidKlug

Faculty of Natural Sciences, Department of Chemistry

Associate Dean for Enterprise for FoNS & Professor
 
 
 
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Contact

 

+44 (0)20 7594 5806d.klug

 
 
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Location

 

Molecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Valim:2014:10.1021/jp509053q,
author = {Valim, LR and Davies, JA and Jensen, KT and Guo, R and Willison, KR and Spickett, CM and Pitt, AR and Klug, DR},
doi = {10.1021/jp509053q},
journal = {Journal of Physical Chemistry B},
pages = {12855--12864},
title = {Identification and Relative Quantification of Tyrosine Nitration in a Model Peptide Using Two-Dimensional Infrared Spectroscopy},
url = {http://dx.doi.org/10.1021/jp509053q},
volume = {118},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Nitration of tyrosine in proteins and peptides is a post-translationalmodification that occurs under conditions of oxidative stress. It is implicated in a varietyof medical conditions, including neurodegenerative and cardiovascular diseases. However,monitoring tyrosine nitration and understanding its role in modifying biological functionremains a major challenge. In this work, we investigate the use of electron-vibration-vibration(EVV) two-dimensional infrared (2DIR) spectroscopy for the study of tyrosine nitration inmodel peptides. We demonstrate the ability of EVV 2DIR spectroscopy to differentiatebetween the neutral and deprotonated states of 3-nitrotyrosine, and we characterize theirspectral signatures using information obtained from quantum chemistry calculations andsimulated EVV 2DIR spectra. To test the sensitivity of the technique, we use mixed-peptidesamples containing various levels of tyrosine nitration, and we use mass spectrometry toindependently verify the level of nitration. We conclude that EVV 2DIR spectroscopy is ableto provide detailed spectroscopic information on peptide side-chain modifications and todetect nitration levels down to 1%. We further propose that lower nitration levels could be detected by introducing a resonantRaman probe step to increase the detection sensitivity of EVV 2DIR spectroscopy.
AU  - Valim,LR
AU  - Davies,JA
AU  - Jensen,KT
AU  - Guo,R
AU  - Willison,KR
AU  - Spickett,CM
AU  - Pitt,AR
AU  - Klug,DR
DO  - 10.1021/jp509053q
EP  - 12864
PY  - 2014///
SN  - 1520-6106
SP  - 12855
TI  - Identification and Relative Quantification of Tyrosine Nitration in a Model Peptide Using Two-Dimensional Infrared Spectroscopy
T2  - Journal of Physical Chemistry B
UR  - http://dx.doi.org/10.1021/jp509053q
UR  - http://hdl.handle.net/10044/1/26614
VL  - 118
ER  -
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