Imperial College London
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Emeritus Professor David Lane

Faculty of Medicine, Department of Immunology and Inflammation

Emeritus Professor of Haematology
 
 
 
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Contact

 

+44 (0)20 3313 2295d.lane

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lynch:2016:10.1182/blood-2015-09-672014,
author = {Lynch, CJ and Lane, DA},
doi = {10.1182/blood-2015-09-672014},
journal = {Blood},
pages = {1711--1718},
title = {N-linked glycan stabilisation of the VWF A2 domain.},
url = {http://dx.doi.org/10.1182/blood-2015-09-672014},
volume = {127},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Shear forces in the blood trigger a conformational transition in the VWF A2 domain, from its native folded to an unfolded state, in which the cryptic scissile bond (Y1605-M1606) is exposed and can then be proteolysed by ADAMTS13. The conformational transition depends upon a Ca(2+) binding site and a vicinal cysteine disulphide bond. Glycosylation at N1574 has previously been suggested to modulate VWF A2 domain interaction with ADAMTS13 through steric hindrance by the bulky carbohydrate structure. We investigated how the N-linked glycans of the VWF A2 domain affect thermostability and regulate both the exposure of the ADAMTS13 binding sites and the scissile bond. We show by differential scanning fluorimetry that the N-linked glycans thermodynamically stabilise the VWF A2 domain. The essential component of the glycan structure is the first sugar residue (GlcNAc) at the N1574 attachment site. From its crystal structures, N1574-GlcNAc is predicted to form stabilising intradomain interactions with Y1544 and nearby residues. Substitution of the surface exposed Y1544 to aspartic acid is able to stabilise the domain in the absence of glycosylation and to protect against ADAMTS13 proteolysis in both the VWF A2 domain and FLVWF. Glycan stabilisation of the VWF A2 domain acts together with the Ca(2+) binding site and vicinal cysteine disulphide bond to control unfolding and ADAMTS13 proteolysis.
AU  - Lynch,CJ
AU  - Lane,DA
DO  - 10.1182/blood-2015-09-672014
EP  - 1718
PY  - 2016///
SN  - 0006-4971
SP  - 1711
TI  - N-linked glycan stabilisation of the VWF A2 domain.
T2  - Blood
UR  - http://dx.doi.org/10.1182/blood-2015-09-672014
UR  - http://hdl.handle.net/10044/1/53223
VL  - 127
ER  -
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