Imperial College London
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Professor David MacIntyre

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor in Reproduction Systems Medicine
 
 
 
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Contact

 

+44 (0)20 7594 2195d.macintyre Website

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Mitra:2021:rs.3.rs-73123/v1,
author = {Mitra, A and MacIntyre, D and Paraskevaidi, M and Moscicki, A-B and Mahajan, V and Smith, A and Lyons, D and Paraskevaidis, E and Marchesi, J and Bennett, P and Kyrgiou, M},
doi = {rs.3.rs-73123/v1},
publisher = {BioMed Central},
title = {The vaginal microbiota and innate Immunity after local excisional treatment for cervical intraepithelial neoplasia},
url = {http://dx.doi.org/10.21203/rs.3.rs-73123/v1},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - Background: Vaginal microbiota (VMB) are altered in women with cervical intra-epithelial neoplasia (CIN) and associate with disease progression. However, the impact of CIN excision on the VMB and innate immunity is not known. This interventional study aims to explore the impact of CIN excision on the VMB, antimicrobial peptides (AMP) and proinflammatory cytokines. We sampled 103 non-pregnant, premenopausal women at the time of excisional treatment for CIN and at their 6-month follow-up visit. A further 39 untreated controls with normal cytology were also sampled. We used metataxonomics to group vaginal swab samples into community state types (CSTs) and ELISA to quantify cytokine and AMPs levels in matched vaginal secretions. Analyses were performed to compare bacterial composition and immune analyte levels before and after CIN excision and in healthy controls.Results: Women with CIN had significantly higher rates of Lactobacillus species depletion pre-treatment compared to healthy controls (CST IV: 21/103, 20% vs 1/39, 3%, p=0.0081). Excision did not change the VMB composition, with CST IV remaining significantly more prevalent after excision compared to untreated, healthy controls (CST IV: 19/103, 20% vs 1/39, 3%, p=0.0142). Prevotella bivia and Sneathia amnii were significantly higher in samples before treatment compared to untreated controls and Prevotella bivia remained significantly higher amongst the treated, with less Lactobacillus crispatus compared to untreated controls. IL-1 and IL-8 remained significantly elevated pre- (p<0.0001 and p=0.0014 respectively) and post-treatment compared to untreated controls (p<0.0001 and p=0.0035 respectively). Levels of human beta-defensin-1 and secretory leukocyte protease inhibitor were both significantly reduced following CIN excision (p<0.0001), however their levels remained lower than controls post-treatment.Conclusions: Women with CIN have increased prevalence of Lactobacillus spp. depleted, high-diversity V
AU  - Mitra,A
AU  - MacIntyre,D
AU  - Paraskevaidi,M
AU  - Moscicki,A-B
AU  - Mahajan,V
AU  - Smith,A
AU  - Lyons,D
AU  - Paraskevaidis,E
AU  - Marchesi,J
AU  - Bennett,P
AU  - Kyrgiou,M
DO  - rs.3.rs-73123/v1
PB  - BioMed Central
PY  - 2021///
TI  - The vaginal microbiota and innate Immunity after local excisional treatment for cervical intraepithelial neoplasia
UR  - http://dx.doi.org/10.21203/rs.3.rs-73123/v1
UR  - https://www.researchsquare.com/article/rs-73123/v1
UR  - http://hdl.handle.net/10044/1/92495
ER  -
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