Imperial College London
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Professor David MacIntyre

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor in Reproduction Systems Medicine
 
 
 
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Contact

 

+44 (0)20 7594 2195d.macintyre Website

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Raglan:2021:rs.3.rs-31292/v3,
author = {Raglan, O and MacIntyre, D and Mitra, A and Lee, Y and Smith, A and Assi, N and Nautiyal, J and Purkayastha, S and Gunter, M and Gabra, H and Marchesi, J and Bennett, P and Kyrgiou, M},
doi = {rs.3.rs-31292/v3},
journal = {Microbiome},
title = {The association between obesity and weight loss after bariatric surgery on the vaginal microbiota},
url = {http://dx.doi.org/10.21203/rs.3.rs-31292/v3},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <h4>Background: </h4> Obesity and vaginal microbiome (VMB) dysbiosis are each risk factors for adverse reproductive and oncological health outcomes in women. Here we investigated the relationship between obesity, vaginal bacterial composition, local inflammation and bariatric surgery. <h4>Methods: </h4>: Vaginal bacterial composition assessed by high-throughput sequencing of bacterial 16S rRNA genes and local cytokine levels measured using a multiplexed Magnetic Luminex Screening Assay were compared between 67 obese and 42 non-obese women. We further assessed temporal changes in the microbiota and cytokines in a subset of 27 women who underwent bariatric surgery. <h4>Results: </h4>: The bacterial component of the vaginal microbiota in obese women was characterised by a lower prevalence of a Lactobacillus -dominant VMB and higher prevalence of a high diversity ( Lactobacillus spp., and Gardnerella - spp. depleted) VMB, compared with non-obese subjects (p<0.001). Obese women had higher relative abundance of Dialister species (p<0.001), Anaerococcus vaginalis (p=0.021) and Prevotella timonensis (p=0.020) and decreased relative abundance of Lactobacillus crispatus (p=0.014). Local vaginal IL-1β, IL-4, IL-6, IL-8, IFNγ, MIP-1α, and TNFα levels were all higher among obese women, however only IL-1β and IL-8 correlated with VMB species diversity. In a subset of obese women undergoing bariatric surgery, there were no significant overall differences in VMB following surgery, however 75% of these women remained obese at six months. Prior to surgery there was no relationship between body mass index (BMI) and VMB structure, however post-surgery women with a Lactobacillus -dominant VMB had a significantly lower BMI than those with a high diversity VMB. <h4>Conclusions: </h4>: Obese women have a significantly different vaginal microbiota composition with increased levels of local inflammation compare
AU  - Raglan,O
AU  - MacIntyre,D
AU  - Mitra,A
AU  - Lee,Y
AU  - Smith,A
AU  - Assi,N
AU  - Nautiyal,J
AU  - Purkayastha,S
AU  - Gunter,M
AU  - Gabra,H
AU  - Marchesi,J
AU  - Bennett,P
AU  - Kyrgiou,M
DO  - rs.3.rs-31292/v3
PY  - 2021///
SN  - 2049-2618
TI  - The association between obesity and weight loss after bariatric surgery on the vaginal microbiota
T2  - Microbiome
UR  - http://dx.doi.org/10.21203/rs.3.rs-31292/v3
ER  -
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