Imperial College London
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Professor David MacIntyre

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor in Reproduction Systems Medicine
 
 
 
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Contact

 

+44 (0)20 7594 2195d.macintyre Website

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Grewal:2022:10.1186/s12916-021-02227-7,
author = {Grewal, K and Lee, Y and Smith, A and Brosens, J and Bourne, T and Al-Memar, M and Kundu, S and MacIntyre, D and Bennett, P},
doi = {10.1186/s12916-021-02227-7},
journal = {BMC Medicine},
title = {Chromosomally normal miscarriage is associated with vaginal dysbiosis and local inflammation},
url = {http://dx.doi.org/10.1186/s12916-021-02227-7},
volume = {20},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundEmerging evidence supports an association between vaginal microbiota composition and risk of miscarriage, however the underlying mechanisms are poorly understood. We aim to investigate the vaginal microbial composition and the local immune response in chromosomally normal and abnormal miscarriages and compare this to uncomplicated pregnancies delivering at term.Methods We used 16S rRNA gene based metataxonomics to interrogate the vaginal microbiota in a cohort of 167 women, 93 miscarriages (54 euploid and 39 aneuploid using molecular cytogenetics) and 74 women who delivered at term and correlate this with the aneuploidy status of the miscarriages. We also measured the concentrations of IL-2, IL-4, IL-6, IL-8, TNF-α, IFN-γ, IL-1β, IL-18 and IL-10 in cervical vaginal fluid.Results We show that euploid miscarriage is associated with a significantly higher prevalence of Lactobacillus spp. deplete vaginal microbial communities compared to aneuploid miscarriage (P=0.01). Integration of matched cervicovaginal fluid immune-profiles showed that Lactobacillus spp. depleted vaginal microbiota associated with pro-inflammatory cytokine levels most strongly in euploid miscarriage compared to viable term pregnancy (IL-1β; P<0.001, IL-8; P=0.01, IL-6; P<0.001). ConclusionOur data suggest the vaginal microbiota plays an important aetiological role in euploid miscarriage and may represent a target to modify risk of pregnancy loss.
AU  - Grewal,K
AU  - Lee,Y
AU  - Smith,A
AU  - Brosens,J
AU  - Bourne,T
AU  - Al-Memar,M
AU  - Kundu,S
AU  - MacIntyre,D
AU  - Bennett,P
DO  - 10.1186/s12916-021-02227-7
PY  - 2022///
SN  - 1741-7015
TI  - Chromosomally normal miscarriage is associated with vaginal dysbiosis and local inflammation
T2  - BMC Medicine
UR  - http://dx.doi.org/10.1186/s12916-021-02227-7
UR  - https://bmcmedicine.biomedcentral.com/articles/10.1186/s12916-021-02227-7
UR  - http://hdl.handle.net/10044/1/93835
VL  - 20
ER  -
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